Tle3 (NM_001083927) Mouse Tagged ORF Clone

CAT＃: MR210620

TrueORF^®

Tle3 (Myc-DDK-tagged) - Mouse transducin-like enhancer of split 3, homolog of Drosophila E(spl) (Tle3), transcript variant 1

Datasheet

SDS

Plasmid Map

"NM_001083927" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 5,736.00

CNY 6,840.00

货期*

现货

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2610103N05Rik; ESG; Grg3a; Grg3b; mKIAA1547
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR210620 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATCCGCAAGGCAGACATCCGGCACCCCATCAACCCGGGCAGCCGGGATTTAAATTCACTGTGGCCG AGTCCTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTA TGACAAGCTGGCTAACGAGAAGACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGC TTGAATATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATCCTAGCCCAGATCATGC CTTTTTTGTCACAGGAGCATCAGCAGCAAGTGGCGCAGGCTGTGGAACGCGCCAAGCAGGTCACCATGAC GGAGTTGAACGCCATCATCGGGGTACGTGGACTCCCCAATCTGCCTCTCACCCAGCAGCAACTCCAGGCC CAGCACCTCTCCCATGCCACGCATGGTCCCCCGGTCCAGCTGCCACCCCACCCGTCAGGCCTCCAGCCTC CTGGGATTCCCCCAGTGACAGGAAGCAGCTCTGGGTTGCTGGCACTTGGTGCCCTGGGAAGTCAAGCTCA CTTGGCGGTGAAGGATGAGAAGAACCACCATGAACTGGATCACAGAGAGAGAGAGTCCAGCACGAACAAT TCCGTGTCACCCTCTGAAAGCCTCCGGGCCAGTGAGAAGCACCGGGGCTCTGCAGACTACAGCATGGAAG CCAAGAAGCGGAAGGCGGAAGAGAAAGACAGCCTCAGCAGATACGATAGCGATGGGGACAAGAGTGACGA CCTGGTGGTGGATGTCTCTAATGAGGACCCAGCAACACCCCGGGTGAGCCCAGCACACTCCCCTCCTGAA AATGGGCTGGACAAAGCCCGTGGTCTGAAGAAAGATGCCCCCACCAGCCCAGCCTCCGTGGCTTCCTCCA GCAGCACACCTTCCTCCAAGACCAAAGACCTTGGTCATAATGACAAATCTTCCACACCTGGGCTCAAGTC CAACACACCAACGCCAAGAAATGATGCCCCAACTCCAGGCACCAGCACCACCCCGGGACTCCGGTCAATG CCGGGCAAACCTCCAGGCATGGACCCGATAGGTATAATGGCCTCGGCCCTGCGAACACCCATCACCCTCA CCAGCTCCTATCCAGCACCCTTTGCCATGATGAGCCACCACGAGATGAATGGCTCCCTCACCAGCCCAAG CGCCTATGCTGGCCTACACAACATCCCATCCCAGATGAGCGCCGCCGCAGCCGCTGCAGCCGCCGCCTAT GGCCGATCGCCAATGGTGAGCTTTGGAGCTGTTGGTTTTGACCCTCACCCCCCAATGAGGGCCACAGGCC TGCCTTCCAGTCTCGCCTCCATTCCTGGAGGGAAACCGGCATACTCCTTCCATGTGAGTGCTGATGGGCA GATGCAACCTGTGCCCTTCCCCCATGATGCACTAGCAGGCCCTGGCATTCCCAGGCATGCCCGGCAGATC AATACGCTCAGCCATGGAGAGGTGGTATGTGCTGTGACCATCAGCAACCCCACACGACACGTCTACACAG GCGGCAAGGGCTGTGTGAAGATATGGGACATCAGCCAGCCGGGCAGCAAGAGTCCCATCTCCCAGCTGGA CTGCCTGAACAGGGACAACTACATCCGCTCGTGCAAGCTTCTCCCCGATGGGCGCACGCTCATTGTGGGT GGTGAGGCCAGCACGCTCACCATCTGGGACCTGGCCTCACCCACACCCCGCATCAAGGCTGAGCTGACGT CCTCGGCTCCAGCCTGTTATGCCCTGGCCATCAGTCCTGATGCCAAAGTCTGTTTTTCCTGCTGCAGCGA CGGGAACATTGCGGTTTGGGATCTGCACAACCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGATGGG GCCAGCTGTATAGACATCTCTCATGATGGCACTAAGCTGTGGACCGGGGGCCTGGACAACACCGTGCGCT CCTGGGACCTACGTGAAGGACGGCAGTTACAGCAACACGATTTCACCTCCCAGATCTTCTCCCTGGGTTA CTGCCCCACTGGGGAGTGGCTGGCCGTGGGCATGGAGAGCAGCAATGTGGAGGTCCTGCACCACACTAAG CCCGACAAATACCAGCTGCACCTGCACGAGAGCTGCGTGCTGTCCCTCAAGTTCGCCTATTGTGGCAAGT GGTTTGTGAGCACTGGGAAAGACAACCTTCTCAATGCCTGGAGGACGCCTTATGGAGCCAGCATCTTCCA GTCAAAAGAATCCTCATCTGTCTTGAGCTGTGACATTTCAGCGGATGACAAATATATTGTAACAGGCTCT GGTGACAAGAAGGCCACAGTTTACGAGGTCATCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR210620 protein sequence Red=Cloning site Green=Tags(s) MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYG LNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGVRGLPNLPLTQQQLQA QHLSHATHGPPVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLAVKDEKNHHELDHRERESSTNN SVSPSESLRASEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPE NGLDKARGLKKDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSM PGKPPGMDPIGIMASALRTPITLTSSYPAPFAMMSHHEMNGSLTSPSAYAGLHNIPSQMSAAAAAAAAAY GRSPMVSFGAVGFDPHPPMRATGLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPGIPRHARQI NTLSHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVG GEASTLTIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDG ASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTK PDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGS GDKKATVYEVIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_001083927
ORF Size	2349 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001083927.1, NP_001077396.1
RefSeq Size	5202 bp
RefSeq ORF	2349 bp
Locus ID	21887
UniProt ID	Q08122
MW	84.5 kDa
Gene Summary	Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity). May play an important role during spermatogenesis.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC221568	Tle3 (untagged) - Mouse transducin-like enhancer of split 3, homolog of Drosophila E(spl) (Tle3), transcript variant 1, (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 10,070.00
MR210620L3	Lenti ORF clone of Tle3 (Myc-DDK-tagged) - Mouse transducin-like enhancer of split 3, homolog of Drosophila E(spl) (Tle3), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,740.00
MR210620L4	Lenti ORF clone of Tle3 (mGFP-tagged) - Mouse transducin-like enhancer of split 3, homolog of Drosophila E(spl) (Tle3), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,136.00 CNY 8,740.00

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DDK/Flag Antibody

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Proteins

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