Ecel1 (NM_021306) Mouse Tagged ORF Clone
CAT#: MR210577
- TrueORF®
Ecel1 (Myc-DDK-tagged) - Mouse endothelin converting enzyme-like 1 (Ecel1)
ORF Plasmid: tGFP
"NM_021306" in other vectors (4)
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CNY 5,688.00
CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DINE; xce |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210577 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCCCGTATTCCATGACAGCGCACTACGACGAGTTCCAGGAAGTCAAGTATGTGAGCCGGTGTG GCACCGGGGGCGCTCGAGGGACCTCACTGCCTCCAGGCTTCCCGAGGGGCTCAGGGCGCAGTGCTAGCGG GTCCAGGTCTGGGCTGCCCCGCTGGAATCGACGCGAGGTGTGCCTGCTGTCGGGGTTGGTGTTTGCTGCC GGCCTCTGCGCCATCCTGGCGGCCATGCTGGCGCTCAAATACCTGGGCCCGGGAGCGGCGGGCGGTGGCG GAGCCTGTCCCGAGGGCTGCCCCGAGCGCAAGGCCTTTGCACGCGCCGCCCGCTTTCTGTCTGCCAACCT GGACGCCAGCATCGACCCGTGCCAGGACTTCTACTCGTTCGCGTGCGGAGGCTGGCTGCGGCGCCACGCT ATCCCCGACGACAAGCTCACCTACGGCACGATCGCGGCCATCGGAGAGCAGAACGAGGAGCGTCTGCGGC GCCTGCTGGCGAGACCCACCGGAGGCCCCGGCGGCGCTGCGCAGCGCAAGGTGCGCGCCTTCTTCCGCTC GTGCCTCGACATGCGCGAGATCGAGAGGCTCGGGCCGCGGCCCATGCTCGAGGTCATCGAGGACTGCGGC GGCTGGGACCTGGGCGGCGCGGCCGATCGCCCCGGGGCGGCTCGCTGGGACCTCAACCGACTGCTGTACA AGGCACAGGGTGTGTACAGCGCGGCCGCGCTCTTCTCGCTTACAGTCAGCCTGGACGACAGGAACTCCTC GCGCTACGTCATCCGCATTGACCAGGATGGGCTCACCCTGCCAGAGAGAACCCTGTACCTAGCTCAAGAC GAGGAGAGTGAGAAGATCTTGGCTGCATACAGGGTGTTCATGCAGCGTCTACTCAGACTACTGGGAGCGG ACGCTGTGGAGCAGAAGGCGCAGGAAATCCTTCAGCTGGAACAGAGGCTGGCCAATATCTCTGTGTCAGA ATATGATGATCTCCGTCGTGATGTCAGCTCCGCGTACAACAAAGTGACTCTGGGGCAGCTGCAGAAGATC ATCCCCCATTTGCAGTGGAAGTGGCTGCTGGATCAGATCTTCCAGGAAGACTTCTCGGAGGAGGAGGAGG TGGTGCTGCTGGCCACAGACTACATGCAGCAGGTGTCCCAGCTCATCCGCTCCACACCTCGAAGGATCCT GCACAACTACCTAGTGTGGCGTGTGGTGGTGGTTCTGAGTGAGCACCTGTCCTCACCATTCCGAGAGGCA CTGCATGAGCTGGCCAAAGAGATGGAGGGCAATGACAAGCCCCAGGAGTTGGCCCGAGTCTGCCTGGGCC AGGCCAACCGCCACTTCGGCATGGCTCTTGGTGCCCTCTTTGTACATGAACACTTCTCAGCTGCCAGTAA AGCCAAGGTGCAGCAGCTAGTGGAAGATATCAAGTACATCTTGGGCCAGCGCCTGGAGGAGCTGGACTGG ATGGATGCCCAGACCAAGGCAGCTGCTCGGGCCAAGCTCCAGTACATGATGGTCATGGTTGGCTATCCAG ACTTCCTGCTGAAACCTGAGGCTGTAGACAAAGAATATGAGTTCGAGGTCCACGAGAAGACCTACTTCAA GAACATTTTGAACAGCATCCGATTCAGTATCCAGTTGTCCGTCAAAAAGATTCGACAGGAGGTGGACAAA TCCTCGTGGCTCCTCCCACCACAGGCGCTCAATGCCTACTATCTGCCCAACAAGAATCAAATGGTCTTCC CAGCTGGCATCCTGCAGCCCACCCTGTATGACCCAGACTTCCCACAGTCTCTGAACTACGGGGGTATTGG CACCATCATTGGGCACGAACTGACCCATGGCTATGACGACTGGGGGGGACAGTATGACCGCTCAGGAAAC CTGTTGCACTGGTGGACTGAGACCTCCTACAGCCACTTTCTGCGCAAGGCCGAGTGCATCGTTCGCCTCT ATGACAACTTCACCGTCTACAACCAGCGCGTGAATGGGAAACACACACTTGGCGAGAACATCGCAGACAT GGGAGGCCTCAAGCTGGCCTACTATGCCTATCAGAAGTGGGTCCGGGAGCATGGCCCGGAGCACCCGCTG CACCGGCTCAAGTACACACACAACCAGCTTTTCTTCATTGCCTTTGCACAGAACTGGTGCATCAAGCGAC GGTCACAGTCCATCTACCTGCAGGTGCTGACAGACAAGCATGCACCTGAGCACTACCGGGTCCTGGGCAG CGTATCCCAGTTTGAGGAATTCGGCCGGGCCTTCCACTGTCCCAAGGACTCTCCCATGAACCCCGTCCAT AAGTGCTCTGTGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210577 protein sequence
Red=Cloning site Green=Tags(s) MEAPYSMTAHYDEFQEVKYVSRCGTGGARGTSLPPGFPRGSGRSASGSRSGLPRWNRREVCLLSGLVFAA GLCAILAAMLALKYLGPGAAGGGGACPEGCPERKAFARAARFLSANLDASIDPCQDFYSFACGGWLRRHA IPDDKLTYGTIAAIGEQNEERLRRLLARPTGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCG GWDLGGAADRPGAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQD EESEKILAAYRVFMQRLLRLLGADAVEQKAQEILQLEQRLANISVSEYDDLRRDVSSAYNKVTLGQLQKI IPHLQWKWLLDQIFQEDFSEEEEVVLLATDYMQQVSQLIRSTPRRILHNYLVWRVVVVLSEHLSSPFREA LHELAKEMEGNDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDW MDAQTKAAARAKLQYMMVMVGYPDFLLKPEAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDK SSWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGN LLHWWTETSYSHFLRKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYYAYQKWVREHGPEHPL HRLKYTHNQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPVH KCSVW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021306 |
ORF Size | 2328 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021306.3 |
RefSeq Size | 2898 bp |
RefSeq ORF | 2328 bp |
Locus ID | 13599 |
UniProt ID | Q9JMI0 |
MW | 88 kDa |
Gene Summary | May contribute to the degradation of peptide hormones and be involved in the inactivation of neuronal peptides.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205157 | Ecel1 (untagged) - Mouse endothelin converting enzyme-like 1 (Ecel1), (10ug) |
CNY 5,688.00 |
|
MG210577 | Ecel1 (tGFP-tagged) - Mouse endothelin converting enzyme-like 1 (Ecel1) |
CNY 6,370.00 |
|
MR210577L3 | Lenti ORF clone of Ecel1 (Myc-DDK-tagged) - Mouse endothelin converting enzyme-like 1 (Ecel1) |
CNY 7,700.00 |
|
MR210577L4 | Lenti ORF clone of Ecel1 (mGFP-tagged) - Mouse endothelin converting enzyme-like 1 (Ecel1) |
CNY 7,700.00 |