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C2 (NM_013484) Mouse Tagged ORF Clone
CAT＃: MR210516
- TrueORF®
C2 (Myc-DDK-tagged) - Mouse complement component 2 (within H-2S) (C2)
ORF Plasmid: tGFP
"NM_013484" in other vectors (4)
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CNY 5,576.00
CNY 6,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210516 representing NM_013484.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCCCTCTGCTGGCTCTCTTCTACCTGCTGCAGCTGGGCCCAGGCCTGGCTGCTCTCTTCTGCAAC CAGAATGTCAATATCACCGGTGGTAATTTCACCCTCAGCCATGGCTGGGCCCCTGGGAGCCTCCTCATC TACTCCTGCCCCCTGGGCAGGTACCCGTCCCCAGCCTGGAGGAAATGTCAGAGCAACGGACAGTGGCTG ACACCAAGGTCTAGCTCACATCACACCCTGCGATCCTCTCGGATGGTTAAAGCAGTCTGCAAACCGGTT CGATGCCTAGCTCCTTCATCCTTTGAAAATGGCATCTATTTCCCTCGGCTGGTGTCCTACCCTGTGGGT AGCAACGTGAGCTTTGAGTGTGAGCAAGACTTCACCTTGCGGGGCTCACCTGTGCGGTACTGTCGCCCC AACGGCCTGTGGGATGGAGAGACGGCTGTGTGTGACAATGGGGCTAGCCACTGCCCCAACCCTGGCATC TCAGTGGGCACAGCTCGGACAGGCTTGAACTTTGACCTTGGGGACAAGGTCAGGTACCGCTGCTCCTCC TCAAATATGGTATTGACTGGCTCTGCAGAGCGGGAGTGTCAGAGCAATGGAGTGTGGAGTGGGTCGGAA CCCATTTGCCGACAGCCTTACTCTTACGACTTCCCTGAGGATGTAGCATCTGCCCTAGACACCTCCCTC ACCAACCTGCTTGGAGCCACCAATCCCACCCAGAACCTTCTGACAAAAAGTTTGGGCCGTAAGATCATA ATCCAGCGCTCGGGTCACCTGAACCTCTATTTGCTGCTTGATGCTTCTCAGAGTGTGACAGAAAAAGAC TTTGACATCTTCAAGAAGAGTGCCGAACTCATGGTGGAGAGGATCTTCAGCTTTGAGGTAAATGTCAGC GTAGCTATCATCACATTTGCCTCTCAGCCCAAAACCATCATGTCGATCCTGAGTGAGAGATCCCAGGAT GTGACGGAGGTGATCACCAGTCTGGACTCTGCCAGCTACAAAGATCACGAAAATGCCACTGGCACTAAC ACTTATGAGGTTCTCATCCGCGTTTACTCCATGATGCAAAGCCAGATGGATCGCCTGGGCATGGAGACC TCTGCCTGGAAGGAAATCCGTCACACCATCATCCTTCTGACTGACGGAAAGTCCAACATGGGTGACTCT CCCAAGAAAGCAGTCACCAGAATCAGAGAGCTCCTGAGCATCGAACAGAACAGAGATGACTACCTGGAC ATCTATGCTATTGGGGTGGGCAAGCTGGATGTGGACTGGAAAGAACTGAATGAGCTGGGTTCCAAGAAG GATGGCGAGAGGCATGCCTTCATCTTGCAGGATGCAAAGGCCTTGCAACAGATCTTTGAGCACATGTTG GATGTCTCTAAGCTCACAGATACCATCTGTGGGGTGGGGAACATGTCCGCCAATGCCTCTGACCAGGAG AGGACACCTTGGCAAGTCACCTTTAAGCCCAAGAGCAAGGAAACTTGCCAGGGATCACTCATCTCTGAT CAGTGGGTGCTGACAGCAGCTCACTGCTTCCATGACATTCAGATGGAGGACCACCACCTGTGGAGGGTC AATGTAGGTGATCCCACCTCTCAGCATGGCAAAGAATTTCTTGTGGAGGACGTGATAATTGCCCCAGGG TTTAATGTCCATGCAAAGCGGAAGCAGGGCATCTCAGAGTTCTATGCTGATGACATTGCCTTGCTGAAG CTATCTCGGAAAGTGAAAATGTCCACCCATGCCAGACCCATCTGCCTTCCTTGCACTGTGGGAGCCAAC ATGGCTCTGCGGAGATCCCCAGGTAGTACCTGTAAAGATCATGAGACAGAACTTCTGTCACAGCAGAAA GTTCCTGCACATTTTGTAGCTTTGAATGGGAACAGACTCAACATCAACCTCAGGACAGGACCTGAGTGG ACAAGGTGTATCCAGGCTGTCTCCCAAAACAAAAACATCTTCCCCAGCTTGACAAACGTTAGCGAGGTG GTGACAGACCAGTTCCTATGCAGTGGGATGGAGGAGGAAGATGACAATCCTTGCAAAGGAGAATCTGGG GGAGCCGTTTTCCTTGGACGGAGATACAGGTTCTTCCAGGTGGGCCTGGTGAGTTGGGGTCTTTTTGAC CCTTGTCATGGTTCCTCCAACAAAAACTTGCGCAAGAAACCTCCACGTGGTGTTCTGCCAAGGGACTTC CACATTAGTCTTTTCCGCCTGCAGCCCTGGCTGAGGCAGCACCTGGATGGTGTCCTGGACTTTCTGCCA CTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by MR210516
Blue=ORF Red=Cloning site Green=Tag(s) MAPLLALFYLLQLGPGLAALFCNQNVNITGGNFTLSHGWAPGSLLIYSCPLGRYPSPAWRKCQSNGQWL TPRSSSHHTLRSSRMVKAVCKPVRCLAPSSFENGIYFPRLVSYPVGSNVSFECEQDFTLRGSPVRYCRP NGLWDGETAVCDNGASHCPNPGISVGTARTGLNFDLGDKVRYRCSSSNMVLTGSAERECQSNGVWSGSE PICRQPYSYDFPEDVASALDTSLTNLLGATNPTQNLLTKSLGRKIIIQRSGHLNLYLLLDASQSVTEKD FDIFKKSAELMVERIFSFEVNVSVAIITFASQPKTIMSILSERSQDVTEVITSLDSASYKDHENATGTN TYEVLIRVYSMMQSQMDRLGMETSAWKEIRHTIILLTDGKSNMGDSPKKAVTRIRELLSIEQNRDDYLD IYAIGVGKLDVDWKELNELGSKKDGERHAFILQDAKALQQIFEHMLDVSKLTDTICGVGNMSANASDQE RTPWQVTFKPKSKETCQGSLISDQWVLTAAHCFHDIQMEDHHLWRVNVGDPTSQHGKEFLVEDVIIAPG FNVHAKRKQGISEFYADDIALLKLSRKVKMSTHARPICLPCTVGANMALRRSPGSTCKDHETELLSQQK VPAHFVALNGNRLNINLRTGPEWTRCIQAVSQNKNIFPSLTNVSEVVTDQFLCSGMEEEDDNPCKGESG GAVFLGRRYRFFQVGLVSWGLFDPCHGSSNKNLRKKPPRGVLPRDFHISLFRLQPWLRQHLDGVLDFLP L TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013484 |
| ORF Size | 2280 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 2644 bp |
| RefSeq ORF | 2283 bp |
| Locus ID | 12263 |
| UniProt ID | P21180 |
| MW | 84.7 kDa |
| Gene Summary | This gene encodes component C2 of the classical pathway of the complement system. The encoded protein undergoes proteolytic processing mediated by component C1 resulting in C2a and C2b fragments. C2a fragment, in turn, selectively cleaves components C3 and C5 of the complement system. Mice lacking the encoded protein are found to be more susceptible to bacterial infections. Mutations in the human homolog of this gene are associated with disorders such as systemic lupus erythematosus, Henoch-Schonlein purpura, or polymyositis. [provided by RefSeq, Mar 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203786 | C2 (untagged) - Mouse complement component 2 (within H-2S) (C2), (10ug) |
CNY 2,000.00 |
|
| MG210516 | C2 (tGFP-tagged) - Mouse complement component 2 (within H-2S) (C2) |
CNY 7,320.00 |
|
| MR210516L3 | Lenti ORF clone of C2 (Myc-DDK-tagged) - Mouse complement component 2 (within H-2S) (C2) |
CNY 8,550.00 |
|
| MR210516L4 | Lenti ORF clone of C2 (mGFP-tagged) - Mouse complement component 2 (within H-2S) (C2) |
CNY 8,550.00 |
