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Mapk7 (NM_011841) Mouse Tagged Lenti ORF Clone
CAT＃: MR210486L3
- LentiORF®
Lenti ORF clone of Mapk7 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 7 (Mapk7)
"NM_011841" in other vectors (3)
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CNY 7,700.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BMK-1; BMK1; ERK-5; ERK5; Erk5-T; PRKM7 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR210486).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011841 |
| ORF Size | 2418 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011841.1, NP_035971.1 |
| RefSeq Size | 2945 bp |
| RefSeq ORF | 2421 bp |
| Locus ID | 23939 |
| UniProt ID | Q9WVS8 |
| Gene Summary | Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression (By similarity). Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221757 | Mapk7 (untagged) - Mouse mitogen-activated protein kinase 7 (Mapk7), (10ug) |
CNY 10,360.00 |
|
| MR210486 | Mapk7 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 7 (Mapk7) |
CNY 5,904.00 |
|
| MR210486L4 | Lenti ORF clone of Mapk7 (mGFP-tagged) - Mouse mitogen-activated protein kinase 7 (Mapk7) |
CNY 7,700.00 |
