United States
Germany
Japan
United Kingdom
China
Cul2 (BC026779) Mouse Tagged ORF Clone
CAT＃: MR210430
- TrueORF®
Cul2 (Myc-DDK-tagged) - Mouse cullin 2 (cDNA clone MGC:25783 IMAGE:4018298)
ORF Plasmid: tGFP
"BC026779" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,456.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1300003D18Rik; 4932411N15Rik; AI327301; mKIAA4106 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210430 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTAAAACCAAGAGTAGTGGATTTTGATGAGACATGGAACAAACTCCTAACTACAATCAAAGCTG TTGTTATGTTGGAATATGTCGAGCGAGCAACATGGAATGACCGCTTCTCAGATATCTATGCTTTATGTGT GGCCTATCCTGAACCACTTGGAGAAAGACTTTATGCGGAAACAAAGATTTTTTTGGAAAGTCACGTTCGG CATTTGTATAAGAGAGTTCTGGAGTCTGAAGAACAAGTGCTTGTTATGTATCATAGGTACTGGGAAGAAT ACAGCAAAGGCGCCGACTATATGGACTGCTTATATAGGTATCTCAATACCCAGTATATTAAAAAGAATAA ATTGACAGAAGCTGATATACAGTATGGCTATGGTGGAGTAGATATGAATGAACCACTTATGGAAATAGGA GAGCTAGCATTGGATATGTGGAGGAAGTTAATGGTTGAACCCCTTCAGAACATCCTCATCCGGATGCTGC TTCGAGAAATTAAAAATGATCGTGGTGGTGAAGATCCAAACCAGAAAGTAATCCATGGGGTTATTAACTC CTTTGTTCATGTTGAACAGTATAAGAAAAAATTTCCCTTAAAGTTTTATCAGGGAATATTCGTGTCTCCC TTTCTGACTGAAACGGGAGAATATTACAAGCAAGAAGCTTCAAATTTATTACAAGAATCAAATTGTTCAC AGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATTAGATGTCGAAAATATTTGCATCCAAG TTCATATACTAAAGTGATTCATGAGTGTCAGCAGCGGATGGTAGCAGACCACTTACAGTTCTTACATTCA GAGTGCCACAGCATCATTCAGCAAGAGAGGAAGAACGACATGGCAAATATGTACGTCTTACTCCGTGCTG TGTCCAGTGGTTTACCTCATATGATTGAGGAGCTGCAGAAACATATCCATGACGAGGGCCTCAGAGCGAC CAGTAACCTTACTCAGGAACATATGCCAACACTATTTGTGGAATCAGTTTTGGAAGTACATGGTAAATTC GTTCAACTTATCAACACTGTTTTGAATGGTGATCAGCATTTTATGAGTGCTTTAGATAAGGCCCTTACAT CTGTTGTAAATTACAGAGAACCCAAGTCTGTTTGCAAAGCGCCTGAACTGCTTGCTAAGTACTGTGACAA CTTGCTAAAGAAGTCAGCGAAGGGGATGACTGAAAATGAAGTGGAGGACAAGCTCACTAGCTTCATCACA GTTTTCAAGTACATTGATGACAAGGACGTCTTTCAGAAGTTCTATGCAAGAATGCTAGCAAAACGTCTAA TTCACGGGTTGTCTATGTCTATGGATTCTGAAGAAGCCATGATCAATAAATTAAAGCAAGCCTGTGGTTA TGAGTTTACCAGCAAGCTGCATCGAATGTATACAGACATGAGTGTCAGCGCTGATTTGAACAATAAGTTC AACAATTTCATCAGAAACCAAGACACCGTGATTGACTTGGGGATTAGTTTCCAAATATATGTTCTTCAGG CTGGTGCGTGGCCTCTCACTCAGGCTCCTTCATCTACATTTGCAATTCCCCAAGAGCTAGAGAAAAGTGT ACAGATGTTTGAATTGTTTTATAGCCAGCATTTCAGTGGACGGAAGCTGACATGGTTGCATTATCTCTGT ACAGGTGAAGTTAAAATGAACTATTTGGGAAAACCATACGTAGCTATGGTTACAACCTACCAAATGGCAG TTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGCTACAAAGAACTTCAAGACAGTACCCAGATGAATGA AAAGGAGCTGACCAAAACAATCAAATCATTACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAA GATATCGATGCAGAATCTTCATTTTCATTAAATATGAGCTTCAGCAGTAAGAGAACAAAATTTAAGATTA CTACGTCAATGCAGAAGGATACGCCACAAGAACTGGAACAGACTAGAAGTGCTGTAGATGAGGACCGGAA AATGTATCTTCAAGCTGCTATTGTCCGCATCATGAAAGCACGAAAAGTGCTTCGGCACAACGCCCTCATT CAAGAGGTAATCAGCCAGTCAAGAGCCAGGTTCAATCCCAGTATCAGCATGATTAAGAAGTGCATAGAAG TTCTGATAGACAAACAGTACATCGAGCGCAGCCAAGCATCTGCAGATGAATACAGTTACGTCGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210430 protein sequence
Red=Cloning site Green=Tags(s) MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYAETKIFLESHVR HLYKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQYIKKNKLTEADIQYGYGGVDMNEPLMEIG ELALDMWRKLMVEPLQNILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQGIFVSP FLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHS ECHSIIQQERKNDMANMYVLLRAVSSGLPHMIEELQKHIHDEGLRATSNLTQEHMPTLFVESVLEVHGKF VQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDKLTSFIT VFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKF NNFIRNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLC TGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKE DIDAESSFSLNMSFSSKRTKFKITTSMQKDTPQELEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALI QEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC026779 |
| ORF Size | 2235 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026779, AAH26779 |
| RefSeq Size | 2765 bp |
| RefSeq ORF | 2237 bp |
| Locus ID | 71745 |
| MW | 86.9 kDa |
| Gene Summary | Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (By similarity). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220829 | Cul2 (untagged) - Mouse cullin 2 (cDNA clone MGC:25783 IMAGE:4018298), (10ug) |
CNY 9,120.00 |
|
| MG210430 | Cul2 (tGFP-tagged) - Mouse cullin 2 (cDNA clone MGC:25783 IMAGE:4018298) |
CNY 4,750.00 |
|
| MR210430L3 | Lenti ORF clone of Cul2 (Myc-DDK-tagged) - Mouse cullin 2 (cDNA clone MGC:25783 IMAGE:4018298) |
CNY 6,180.00 |
|
| MR210430L4 | Lenti ORF clone of Cul2 (mGFP-tagged) - Mouse cullin 2 (cDNA clone MGC:25783 IMAGE:4018298) |
CNY 6,180.00 |
