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Fchsd2 (NM_199012) Mouse Tagged ORF Clone
CAT＃: MR210309
- TrueORF®
Fchsd2 (Myc-DDK-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1
ORF Plasmid: tGFP
"NM_199012" in other vectors (4)
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CNY 5,320.00
CNY 6,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BC034086; mKIAA0769; R74866; Sh3md3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210309 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGACTTTCAGTCAGAAGAAGGCTGCTATTGAAAGAGAGTATGCACAGGGTATCCAGAAGTTGGCTA GTCAATACCTGAAGAGAGATTGGCCTGGAATAAAAACTGATGATCGGAATGATTACAGGAGTATGTATCC TGTTTGGAAATCTTTTCTCGAGGGAACAATGCAGGTAGCCCAGTCTCGGATCAATATATGTGAAAACTAT AAAAACTTCATTTCTGAACCTGCAAGAGCAGTAAGAAGCTTAAAAGAACAGCAACTAAAACGGTGTGTGG ATCAGTTGACAAAGATCCAAACAGAATTACAGGAAACTGTGAAAGATTTAGTGAAAGGCAAAAAGAAGTA CTTTGAGACTGAACAGATGGCTCATGCAGTAAGAGAGAAAGCTGACATCGAAGCAAAGTCTAAACTTAGT CTTTTTCAATCAAGAATCAGTTTACAGAAAGCAAGTGTGAAGTTAAAAGCCCGGCGATCTGAATGTAATA CCAAAGCTACCCATGCAAGGAATGATTACCTTCTGACGCTAGCAGCAGCTAACGCACATCAGGACCGCTA CTATCAAACAGACTTAGTTAACATCATGAAGGCTCTTGATGGAAACGTGTATGACCACCTCAAGGATTAT TTAATAGCCTTCAGCCGGACCGAGCTAGAAACTTGCCAAGCTATACAGAATACATTCCAGTTTTTACTAG AAAACTCCAGCAAGGTGGTACGGGACTATAACCTTCAGCTGTTTCTACAAGAGAATGCTGTATTTCACAA GCCTCAGCCCTTCCAGTTCCAGCCTTGTGACAGTGATACTAGTTTAAAAGCCGCCCAACTGGTGGATATT GAGCTCTCCCCTGTCAGTGCTCTGAGAATGACGATAGCTGAGAGTCGACAATTAGAATCAGAAACTGGTA CCACAGAGGAACACAGTCTAAATAAGGAGGCTCGGAAATGGGCCACACGTGTGGCACGTGAGCACAAAAA TATTGTTCATCAGCAGCGGGTTCTAAATGAACTAGAATGTCATGGAGTTGCTCTATCAGAACAGAGCCGA GCAGAGCTGGAACAGAAAATAGATGAAGCAAGAGAAAGTATCCGTAAAGCAGAGATCATTAAATTGAAAG CTGAAGCCCGGCTAGACCTACTGAAGCAGATTGGGGTTTCTGTAGATACGTGGTTAAAGAGTGCCATGAA CCAAGTGATGGAGGAACTAGAAAATGAGCGGTGGGCCCGTCCTCCAGCCGTGACCAGCAATGGCACTCTA CACTCGCTTAATGCAGATGCTGAAAGAGAAGAAGGAGAAGAGTTTGAAGACAACATGGATGTTTTTGATG ACAGCAGCTCCAGCCCTTCTGGCACCTTGAGAAATTATCCACTCACCTGCAAAGTTGTTTATTCCTATAA GGCTTCTCAACCAGATGAGTTGACCATTGAGGAACATGAGGTGTTAGAAGTGATTGAAGATGGAGATATG GAAGACTGGGTAAAGGCTCGAAATAAAGTTGGCCAAGTGGGTTATGTGCCAGAAAAGTACCTACAATTTC CCACCTCGAACAGCCTACTGAGCATGCTACAGTCTTTGGCTGCTTTGGACAGTCGGTCACACACATCCAG CAATTCCACAGAAGCAGAACTCGTTTCAGGCAGCCTGAATGGAGATGCCAGTGTCTGTTTTGTGAAAGCA CTTTATGATTATGAGGGCCAGACAGATGATGAGCTGTCCTTTCCCGAGGGAGCAATTATTCGCATCTTGA ACAAAGAAAACCAAGATGATGATGGCTTCTGGGAAGGGGAGTTCAGTGGTCGAATCGGTGTTTTCCCATC GGTGCTAGTGGAAGAGCTCTCAGCCTCCGAGAATGGCGACACTCCATGGACAAGAGAGATCCAGATCTCT CCATCCCCCAAGCCTCACACATCCCTGCCTCCACTGCCTCTGTATGACCAGCCACCCAGCAGCCCGTATC CCAGTCCAGATAAGAGGAGCTCCCAGTTCTTCCCCCGGTCTCCTTCAGCCAATGAAAACAGCCTTCATGC TGAATCACCAGGATTCTCACAGGCCTCAAGACAGACTCCTGACACCTCATATGGCAAGCTGCGGCCTGTT CGGGCGGCGCCGCCACCACCCACACAGAACCACCGGCGGACAACTGAGAAGATGGAGGACGTGGAGATCA CACTGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210309 protein sequence
Red=Cloning site Green=Tags(s) MRTFSQKKAAIEREYAQGIQKLASQYLKRDWPGIKTDDRNDYRSMYPVWKSFLEGTMQVAQSRINICENY KNFISEPARAVRSLKEQQLKRCVDQLTKIQTELQETVKDLVKGKKKYFETEQMAHAVREKADIEAKSKLS LFQSRISLQKASVKLKARRSECNTKATHARNDYLLTLAAANAHQDRYYQTDLVNIMKALDGNVYDHLKDY LIAFSRTELETCQAIQNTFQFLLENSSKVVRDYNLQLFLQENAVFHKPQPFQFQPCDSDTSLKAAQLVDI ELSPVSALRMTIAESRQLESETGTTEEHSLNKEARKWATRVAREHKNIVHQQRVLNELECHGVALSEQSR AELEQKIDEARESIRKAEIIKLKAEARLDLLKQIGVSVDTWLKSAMNQVMEELENERWARPPAVTSNGTL HSLNADAEREEGEEFEDNMDVFDDSSSSPSGTLRNYPLTCKVVYSYKASQPDELTIEEHEVLEVIEDGDM EDWVKARNKVGQVGYVPEKYLQFPTSNSLLSMLQSLAALDSRSHTSSNSTEAELVSGSLNGDASVCFVKA LYDYEGQTDDELSFPEGAIIRILNKENQDDDGFWEGEFSGRIGVFPSVLVEELSASENGDTPWTREIQIS PSPKPHTSLPPLPLYDQPPSSPYPSPDKRSSQFFPRSPSANENSLHAESPGFSQASRQTPDTSYGKLRPV RAAPPPPTQNHRRTTEKMEDVEITLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199012 |
| ORF Size | 2181 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199012.1, NP_950177.1 |
| RefSeq Size | 4432 bp |
| RefSeq ORF | 2295 bp |
| Locus ID | 207278 |
| UniProt ID | Q3USJ8 |
| MW | 82.4 kDa |
| Gene Summary | Adapter protein that plays a role in endocytosis via clathrin-coated pits. Contributes to the internalization of cell surface receptors, such as integrin ITGB1 and transferrin receptor. Promotes endocytosis of EGFR in cancer cells, and thereby contributes to the down-regulation of EGFR signaling. Recruited to clathrin-coated pits during a mid-to-late stage of assembly, where it is required for normal progress from U-shaped intermediate stage pits to terminal, omega-shaped pits. Binds to membranes enriched in phosphatidylinositol 3,4-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate (By similarity). When bound to membranes, promotes actin polymerization via its interaction with WAS and/or WASL which leads to the activation of the Arp2/3 complex (PubMed:23437151). Does not promote actin polymerisation in the absence of membranes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221418 | Fchsd2 (untagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1, (10ug) |
CNY 9,880.00 |
|
| MG210309 | Fchsd2 (tGFP-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2) |
CNY 7,320.00 |
|
| MR210309L3 | Lenti ORF clone of Fchsd2 (Myc-DDK-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1 |
CNY 8,550.00 |
|
| MR210309L4 | Lenti ORF clone of Fchsd2 (mGFP-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1 |
CNY 8,550.00 |
