Capn1 (NM_007600) Mouse Tagged ORF Clone
CAT#: MR210215
- TrueORF®
Capn1 (Myc-DDK-tagged) - Mouse calpain 1 (Capn1), transcript variant 1
ORF Plasmid: tGFP
"NM_007600" in other vectors (4)
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CNY 5,232.00
CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Capa-1; Capa1; mu-calpin |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210215 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGAGGAGTTAATCACCCCTGTGTACTGCACCGGGGTGTCAGCCCAAGTACAGAAGAAGCGGGACA AGGAGCTGGGCTTGGGCCGCCATGAAAACGCCATCAAGTACCTGGGCCAGGATTATGAAACGCTTCGGGC AAGATGCCTGCAGAGTGGGGTCCTCTTCCAAGACGAGGCCTTCCCTCCGGTTTCTCATAGCCTGGGCTTC AAGGAACTGGGTCCTCATTCCTCTAAAACCTATGGCATCAAATGGAAGCGGCCTACGGAACTGATGTCAA ACCCCCAGTTCATCGTGGATGGAGCCACCCGGACAGACATCTGCCAGGGAGCACTGGGGGACTGTTGGCT CCTGGCTGCCATTGCCTCCCTCACCCTCAACGAGACTATTCTGCACCGAGTGGTTCCCTACGGCCAGAGC TTTCAGGATGGCTACGCTGGCATCTTTCATTTCCAGCTGTGGCAGTTCGGGGAGTGGGTCGACGTGGTCA TAGATGACTTGCTGCCCACCAAGGACGGGAAGCTGGTGTTCGTGCACTCTGCCCAGGGCAACGAGTTCTG GAGCGCACTGCTGGAGAAAGCCTATGCTAAAGTGAATGGCAGCTATGAGGCTCTTTCGGGAGGCTGCACC TCAGAGGCCTTCGAGGACTTTACCGGTGGGGTCACTGAGTGGTACGACCTGCAGAAGGCCCCCAGCGACC TCTACCAGATCATTCTAAAGGCCCTGGAACGAGGCTCCTTGCTGGGCTGCTCCATTAATATCTCCGATAT CCGTGATTTAGAGGCTATTACTTTTAAGAACCTGGTGAGGGGCCATGCGTACTCTGTGACGGGCGCCAAG CAGGTAACTTACCAGGGCCAGCGGGTGAACCTAATCCGGATGCGGAACCCCTGGGGTGAAGTGGAGTGGA AAGGACCCTGGAGTGACAGCTCCTATGAGTGGAACAAAGTGGACCCCTATGAACGAGAGCAGCTGAGGGT CAAGATGGAGGATGGGGAGTTCTGGATGTCGTTCCGAGACTTCATCCGTGAATTCACCAAACTGGAAATC TGCAACCTTACACCGGACGCCCTTAAGAGCAGGACCCTCCGGAATTGGAATACCACATTTTACGAGGGCA CCTGGCGTCGGGGAAGCACCGCTGGAGGCTGCAGGAACTACCCAGCTACCTTCTGGGTAAACCCCCAGTT CAAGATCCGGTTGGAGGAGGTGGATGACGCAGACGACTATGACAACCGGGAGTCGGGCTGCAGCTTCTTG CTGGCCCTCATGCAGAAACACCGCCGCAGGGAGCGTCGCTTTGGCCGGGACATGGAGACCATCGGTTTTG CAGTGTACCAGGTCCCTCGGGAGCTGGCGGGTCAGCCTGTGCACTTGAAGCGTGATTTCTTCCTGGCCAA CGCTTCTCGGGCGCAGTCAGAGCACTTCATCAACCTTCGGGAAGTCAGCAACCGTATCCGCCTGCCGCCC GGGGAGTACATAGTGGTGCCCTCCACCTTCGAGCCCAACAAAGAAGGCGACTTCCTGCTGCGCTTCTTCT CAGAGAAGAAGGCTGGGACCCAGGAACTAGATGACCAGATCCAGGCCAACCTCCCTGATGAGAAAGTTCT CTCTGAAGAGGAGATTGATGACAACTTCAAGACCTTGTTCAGCAAGTTGGCAGGGGATGACATGGAGATC AGCGTCAAGGAGCTACAGACCATCTTGAACAGGATCATTAGCAAACACAAAGACCTGCGCACTAATGGCT TCAGCCTGGAGTCGTGCCGCAGCATGGTGAACCTCATGGATCGAGATGGCAACGGGAAGCTGGGTCTGGT GGAGTTCAACATCCTGTGGAACCGCATCCGAAATTACCTGACCATCTTCCGGAAGTTTGACCTGGACAAG TCTGGCAGCATGAGTGCCTATGAGATGAGGATGGCCATCGAGGCTGCAGGCTTCAAGCTTAACAAGAAGC TGCATGAACTCATAATCACCCGCTACTCGGAGCCCGACCTGGCCGTGGACTTTGACAACTTTGTGTGCTG TCTTGTGCGGCTGGAGACCATGTTCCGGTTTTTCAAACTTCTGGACACAGACCTGGATGGTGTTGTGACC TTTGATCTATTTAAGTGGCTCCAGCTGACTATGTTTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210215 protein sequence
Red=Cloning site Green=Tags(s) MTEELITPVYCTGVSAQVQKKRDKELGLGRHENAIKYLGQDYETLRARCLQSGVLFQDEAFPPVSHSLGF KELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQS FQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCT SEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTGAK QVTYQGQRVNLIRMRNPWGEVEWKGPWSDSSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEI CNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDNRESGCSFL LALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPP GEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEI SVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDK SGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKLLDTDLDGVVT FDLFKWLQLTMFA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007600 |
ORF Size | 2142 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007600.2, NP_031626.1 |
RefSeq Size | 3119 bp |
RefSeq ORF | 2142 bp |
Locus ID | 12333 |
UniProt ID | O35350 |
MW | 82.1 kDa |
Gene Summary | Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220975 | Capn1 (untagged) - Mouse calpain 1 (Capn1), transcript variant 1, (10ug) |
CNY 9,220.00 |
|
MG210215 | Capn1 (tGFP-tagged) - Mouse calpain 1 (Capn1) |
CNY 6,370.00 |
|
MR210215L3 | Lenti ORF clone of Capn1 (Myc-DDK-tagged) - Mouse calpain 1 (Capn1), transcript variant 1 |
CNY 7,700.00 |
|
MR210215L4 | Lenti ORF clone of Capn1 (mGFP-tagged) - Mouse calpain 1 (Capn1), transcript variant 1 |
CNY 7,700.00 |