Ikbke (BC037446) Mouse Tagged ORF Clone

CAT#: MR210080

  • TrueORF®

Ikbke (Myc-DDK-tagged) - Mouse inhibitor of kappaB kinase epsilon (cDNA clone MGC:46849 IMAGE:3980145)

ORF Plasmid: DDK tGFP



  "BC037446" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,080.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Ikki, IKK-i
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210080 representing BC037446
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAGTACCACTAACTACCTGTGGCATACTGATGACCTGCTAGGGCAGGGGGCCACTGCCAGTGTGT
ACAAGGCCCGAAACAAGAAATCCGGGGAGGTGGTTGCTGTAAAGGTCTTCAACTCAGCCAGCTATCGGCG
ACCTCCTGAGGTTCAGGGAGGCAGCCGGCAGAAGGTGCTAATCATGGAGTACTGCTCCAGTGGGAGCCTG
CTGAGCGTGCTGGAAGACCCTGAGAACACGTTCGGGCTTTCTGAAGAGGAGTTCCTAGTGGTGCTGCGCT
GTGTGGTGGCTGGCATGAACCACCTGCGGGAGAATGGCATTGTCCATCGGGACATCAAACCTGGGAACAT
CATGCGCCTGGTGGGCGAGGAGGGGCAGAGCATCTATAAGCTGTCTGACTTCGGGGCTGCCCGCAAGCTG
GACGATGATGAGAAGTTTGTTTCTGTCTATGGTACAGAGGAATACCTGCACCCTGACATGTATGAGCGTG
CAGTGCTGCGCAAACCCCAGCAAAAGGCATTTGGTGTGACTGTGGATCTCTGGAGTATTGGGGTGACCCT
GTACCACGCAGCCACAGGCAGTCTGCCCTTCATCCCCTTCGGTGGGCCCCGGCGCAACAAAGAGATCATG
TACAGAATCACCACAGAGAAGCCAGCCGGGGCCATTTCAGGGACTCAGAAGCAGGAAAATGGTCCCTTGG
AGTGGAGCTACAGCCTCCCCATCACCTGTAGACTGTCCATGGGGCTGCAGAACCAGCTGGTGCCCATCCT
GGCCAACATCCTGGAGGTGGAAGAGGATAAGTGCTGGGGCTTTGATCAGTTCTTCGCGGAGACCAGTGAC
ATTCTGCAGCGAACGGTCATCCACGTCTTTTCCCTACCCCAGGCCGTTTTGCATCATGTCTACATCCACG
CCCACAACACGATTGCCATCTTTTTGGAGGCTGTATATGAGCAGACCAACGTGACCCCCAAACACCAGGA
GTACCTCTTCGAGGGTCACCCTTGTGTCCTTGAGCCAAGCCTCTCAGCCCAGCACATCGCCCACACAGCT
GCCAGCAGCCCTCTAACTCTGTTCAGCATGTCCAGCGACACACCTAAGGGGCTGGCCTTCAGGGACCCTG
CTCTGGATGTCCCAAAGTTCGTCCCTAAGGTTGACCTACAGGCCGATTACAGCACAGCTAAGGGGGTGCT
GGGCGCTGGCTACCAGGCCCTGTGGCTGGCGCGGGTCCTGCTGGATGGACAGGCGTTGATGCTTCGGGGG
TTACATTGGGTCCTGGAGGTGCTTCAGGACACGTGCCAGCAGACACTGGAGGTCACACGGACAGCCCTCC
TCTACCTCGGCAGCAGCCTGGGCACTGAAAGGTTCAGCAGTGGATCGGGGATGCCTGACGTCCAGGAACG
AAAGGAGGCCACAGAGCTAAGAACCAGGCTGCAGACTCTCTCAGAGATCCTGTCTAAATGTTCCCACAAT
GTCACAGAAACCCAAAGGAGCCTGAGCTGTCTGGGTGAAGAGCTTTTAAAGAACCGGGACCAGATTCATG
AGGATAACAAAAGTATCCAGAAGATTCAGTGTTGTTTGGACAAGATGCACTTCATCTACAAACAGTTCAA
GAAATCCAGGATGAGGCCAGGGCTCAGCTACAATGAGGAGCAGATCCACAAGCTGGATAAGGTAAATTTC
AGTCATCTAGCCAAGAGGCTGCTGCAGGTGTTCCAGGAGGAGTGTGTGCAGACGTATCAGGTGTCGCTGG
TCACACACGGCAAGCGGATGAGGCAGGTGCAGAGGGCCCAGAACCACCTGCATCTCATTGGCCACTCTGT
GGCCACCTGTAACTCGGAAGCCCGGGGAGCCCAGGAGAGTCTGAACAAGATCTTTGATCAGCTCCTTCTG
GACAGAGCTTCCGAACAGGGAGCTGAGGTGTCACCGCAACCTATGGCTCCTCATCCCGGCCCTGATCCGA
AGGACCTGGTCTTCCACATGCAGGAGCTTTGTAATGATATGAAGCTATTGGCCTTTGATCTCCAGGACAA
CAACCGACTCATCGAACGGTTACATAGAGTTCCATCGGCACCAGATGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210080 representing BC037446
Red=Cloning site Green=Tags(s)

MQSTTNYLWHTDDLLGQGATASVYKARNKKSGEVVAVKVFNSASYRRPPEVQGGSRQKVLIMEYCSSGSL
LSVLEDPENTFGLSEEEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLSDFGAARKL
DDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIM
YRITTEKPAGAISGTQKQENGPLEWSYSLPITCRLSMGLQNQLVPILANILEVEEDKCWGFDQFFAETSD
ILQRTVIHVFSLPQAVLHHVYIHAHNTIAIFLEAVYEQTNVTPKHQEYLFEGHPCVLEPSLSAQHIAHTA
ASSPLTLFSMSSDTPKGLAFRDPALDVPKFVPKVDLQADYSTAKGVLGAGYQALWLARVLLDGQALMLRG
LHWVLEVLQDTCQQTLEVTRTALLYLGSSLGTERFSSGSGMPDVQERKEATELRTRLQTLSEILSKCSHN
VTETQRSLSCLGEELLKNRDQIHEDNKSIQKIQCCLDKMHFIYKQFKKSRMRPGLSYNEEQIHKLDKVNF
SHLAKRLLQVFQEECVQTYQVSLVTHGKRMRQVQRAQNHLHLIGHSVATCNSEARGAQESLNKIFDQLLL
DRASEQGAEVSPQPMAPHPGPDPKDLVFHMQELCNDMKLLAFDLQDNNRLIERLHRVPSAPDV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC037446
ORF Size 2079 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC037446, AAH37446
RefSeq Size 2931 bp
RefSeq ORF 2081 bp
Locus ID 56489
MW 107.5 kDa
Gene Summary Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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