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Large2 (NM_172670) Mouse Tagged ORF Clone
CAT＃: MR210059
- TrueORF®
Gyltl1b (Myc-DDK-tagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 1
"NM_172670" in other vectors (3)
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CNY 5,056.00
CNY 6,080.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5730485C17Rik; AI891893; Gyltl1b; Largel; mKIAA4105 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210059 representing NM_172670
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCCCGAGGTCGCCCCCGGGCAATGGGGGCCGCCGTGCTGCTGCTGCTGCTGTTGCTAGTGGTTG GCTTCTTCCTGTTCGGCCGGGACCCGGATTATGGACTGGGCACAACTGCTACCCTCGATGAAGACCCGTA CAGGAGTCGCAACCTCTCCGCCTCCAGCCCGCAGCTTCTACTGCCACCCAAGTGCGAGATGTTGCATGTG GCTATCGTGTGTGCGGGATACAACTCCAGCCGAGAGATTATTACCCTAACGAAGTCCCTGCTATTCTACA GGAAAAATCCGCTGCACCTCCACCTGATAACTGATGCCGTAGCCAGAAACATCCTGGAGACACTCTTCCG AACATGGATGGTGCCAGCGGTGGTGGTCAGCTTCTATGATGCGGAAGAACTCAAGCCCCTGGTCTCCTGG ATCCCCAACAAACACTACTCTGGCCTCTATGGGCTAATGAAGCTAGTACTTCCCAGCATCCTGCCTCCCA GCCTGGCCCGAGTCATCGTCCTGGATACCGACGTCACTTTCTCCTCTGACATTGTGGAGCTCTGGGCACT CTTTGATCATTTTTCTGACAAGCAGGTGGTCGGTCTCGTGGAGAACCAGAGCGACTGGTACCTGGGCAAC CTCTGGAAGAACCATAGGCCCTGGCCTGCCTTGGGCAGGGGATTTAACACAGGTGTGATCCTGCTGTGGC TGGACAGGCTCCAGCAAACTGGCTGGGAGCAGATGTGGAAGGTGACAGCCAAACGAGAGCTGCTCACTCT GATGGCTACTTCCTTGGCTGACCAGGACATCTTCAATGCGGTCATCAAGGAGCACCCCCATCTGGTGCAC CCCCTGCCCTGTGTCTGGAACGTGCAGCTGTCAGACCACACTCGGGCTGAGCGCTGCTACCTGGAAGCAG CTGACCTCAAAGTGATCCACTGGAATTCACCAAAGAAGCTTCGAGTGAAGAACAAGCACGCAGAATTCTT CCGTAATCTGCACTTGACCTTTCTGGGGTATGATGGGAAGCTACTGCGAAGAGAGCTCTTTGGATGCCCC AACCAGTTCCCTCCTGGGGCCGAGCAGTTGCAACAGGCCCTAACACAGCTGGATGAGGAAGAGCCCTGCT TTGAGTTCCGCCAACAGCAGCTCACTGTGCACCGGGTGCACATCACCTTCCTGCCCCACCAGCCGCCACC TCCCCAGCCTCACGATGTCACCTTGGTGGCCCAACTCTCTATGGACCGGCTGCAGATGCTGGAAGCCCTG TGCAGGCACTGGCCAGGCCCCATGAGCCTGGCCTTGTACCTGACAGATGAAGAGGCTCAACAATTTCTTC ATTTTGTGGAAACGTCGCCAGTGCTCTCTATGAGGAAGGATGTGGCCTACCATGTAGTGTACCGGGACGG TCCACTCTATCCAGTCAACCAGCTCCGCAACGTGGCCTTGGCCCAGGCTCTCACACCCTACGTCTTCCTC AGTGATATTGACTTCTTACCTGCCTACTCCCTCTACGACTACCTCAGGGCTTCTATCGAGCAGCTGGAGC TGGACAGTCGGCGCAAGACTGCTTTGGTGGTGCCTGCATTTGAGACCCTACACTACCGGTTCAGCTTCCC AAACTCTAAGGCAGAGCTGTTGACGTTACTGGATGCCGGCTCCCTTCACACCTTTAGGTACCACGAGTGG CCACAGGGTCACTCATCCACAGACTATTCCCGCTGGCGGGAAGCCCAGGCACCATACAGTGTGCAGTGGT CAGCTGACTATGAACCCTACGTGGTGGTACCCCGTGACTGCCCCCGTTATGATCCTCGCTTTGTGGGATT TGGCTGGAACAAGGTGGCCCACATCATAGAGTTGGATGCTCAGGAATATGAATTCCTGGTACTTCCTGAG GCCTTCTCTATCCACTTGCCCCACGCTCCAAGTCTTGACATCTCCCGCTTCCGCTCCAGCCCCACCTACC GCAACTGTCTCCAGGCCCTCAAGGAAGAGTTCCACCAGGACTTGTCAAGGCGCTATGGGTCTGCAGCCCT GAAATACCTCACTGCCCTGCAGCAGGCCCGAAGTCGGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210059 representing NM_172670
Red=Cloning site Green=Tags(s) MLPRGRPRAMGAAVLLLLLLLVVGFFLFGRDPDYGLGTTATLDEDPYRSRNLSASSPQLLLPPKCEMLHV AIVCAGYNSSREIITLTKSLLFYRKNPLHLHLITDAVARNILETLFRTWMVPAVVVSFYDAEELKPLVSW IPNKHYSGLYGLMKLVLPSILPPSLARVIVLDTDVTFSSDIVELWALFDHFSDKQVVGLVENQSDWYLGN LWKNHRPWPALGRGFNTGVILLWLDRLQQTGWEQMWKVTAKRELLTLMATSLADQDIFNAVIKEHPHLVH PLPCVWNVQLSDHTRAERCYLEAADLKVIHWNSPKKLRVKNKHAEFFRNLHLTFLGYDGKLLRRELFGCP NQFPPGAEQLQQALTQLDEEEPCFEFRQQQLTVHRVHITFLPHQPPPPQPHDVTLVAQLSMDRLQMLEAL CRHWPGPMSLALYLTDEEAQQFLHFVETSPVLSMRKDVAYHVVYRDGPLYPVNQLRNVALAQALTPYVFL SDIDFLPAYSLYDYLRASIEQLELDSRRKTALVVPAFETLHYRFSFPNSKAELLTLLDAGSLHTFRYHEW PQGHSSTDYSRWREAQAPYSVQWSADYEPYVVVPRDCPRYDPRFVGFGWNKVAHIIELDAQEYEFLVLPE AFSIHLPHAPSLDISRFRSSPTYRNCLQALKEEFHQDLSRRYGSAALKYLTALQQARSRA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172670 |
| ORF Size | 2070 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172670.3, NP_766258.2 |
| RefSeq Size | 2424 bp |
| RefSeq ORF | 2073 bp |
| Locus ID | 228366 |
| UniProt ID | Q5XPT3 |
| MW | 80 kDa |
| Gene Summary | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE (PubMed:15958417).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220532 | Gyltl1b (untagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 1, (10ug) |
CNY 8,840.00 |
|
| MR210059L3 | Lenti ORF clone of Gyltl1b (Myc-DDK-tagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 1 |
CNY 7,980.00 |
|
| MR210059L4 | Lenti ORF clone of Gyltl1b (mGFP-tagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 1 |
CNY 7,980.00 |
