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Large2 (NM_172670) Mouse Untagged Clone
CAT＃: MC220532
Gyltl1b (untagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 1, (10ug)
CNY 8,840.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5730485C17Rik; AI891893; Gyltl1b; Largel; mKIAA4105 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220532 representing NM_172670
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCCCGAGGTCGCCCCCGGGCAATGGGGGCCGCCGTGCTGCTGCTGCTGCTGTTGCTAGTGGTTG GCTTCTTCCTGTTCGGCCGGGACCCGGATTATGGACTGGGCACAACTGCTACCCTCGATGAAGACCCGTA CAGGAGTCGCAACCTCTCCGCCTCCAGCCCGCAGCTTCTACTGCCACCCAAGTGCGAGATGTTGCATGTG GCTATCGTGTGTGCGGGATACAACTCCAGCCGAGAGATTATTACCCTAACGAAGTCCCTGCTATTCTACA GGAAAAATCCGCTGCACCTCCACCTGATAACTGATGCCGTAGCCAGAAACATCCTGGAGACACTCTTCCG AACATGGATGGTGCCAGCGGTGGTGGTCAGCTTCTATGATGCGGAAGAACTCAAGCCCCTGGTCTCCTGG ATCCCCAACAAACACTACTCTGGCCTCTATGGGCTAATGAAGCTAGTACTTCCCAGCATCCTGCCTCCCA GCCTGGCCCGAGTCATCGTCCTGGATACCGACGTCACTTTCTCCTCTGACATTGTGGAGCTCTGGGCACT CTTTGATCATTTTTCTGACAAGCAGGTGGTCGGTCTCGTGGAGAACCAGAGCGACTGGTACCTGGGCAAC CTCTGGAAGAACCATAGGCCCTGGCCTGCCTTGGGCAGGGGATTTAACACAGGTGTGATCCTGCTGTGGC TGGACAGGCTCCAGCAAACTGGCTGGGAGCAGATGTGGAAGGTGACAGCCAAACGAGAGCTGCTCACTCT GATGGCTACTTCCTTGGCTGACCAGGACATCTTCAATGCGGTCATCAAGGAGCACCCCCATCTGGTGCAC CCCCTGCCCTGTGTCTGGAACGTGCAGCTGTCAGACCACACTCGGGCTGAGCGCTGCTACCTGGAAGCAG CTGACCTCAAAGTGATCCACTGGAATTCACCAAAGAAGCTTCGAGTGAAGAACAAGCACGCAGAATTCTT CCGTAATCTGCACTTGACCTTTCTGGGGTATGATGGGAAGCTACTGCGAAGAGAGCTCTTTGGATGCCCC AACCAGTTCCCTCCTGGGGCCGAGCAGTTGCAACAGGCCCTAACACAGCTGGATGAGGAAGAGCCCTGCT TTGAGTTCCGCCAACAGCAGCTCACTGTGCACCGGGTGCACATCACCTTCCTGCCCCACCAGCCGCCACC TCCCCAGCCTCACGATGTCACCTTGGTGGCCCAACTCTCTATGGACCGGCTGCAGATGCTGGAAGCCCTG TGCAGGCACTGGCCAGGCCCCATGAGCCTGGCCTTGTACCTGACAGATGAAGAGGCTCAACAATTTCTTC ATTTTGTGGAAACGTCGCCAGTGCTCTCTATGAGGAAGGATGTGGCCTACCATGTAGTGTACCGGGACGG TCCACTCTATCCAGTCAACCAGCTCCGCAACGTGGCCTTGGCCCAGGCTCTCACACCCTACGTCTTCCTC AGTGATATTGACTTCTTACCTGCCTACTCCCTCTACGACTACCTCAGGGCTTCTATCGAGCAGCTGGAGC TGGACAGTCGGCGCAAGACTGCTTTGGTGGTGCCTGCATTTGAGACCCTACACTACCGGTTCAGCTTCCC AAACTCTAAGGCAGAGCTGTTGACGTTACTGGATGCCGGCTCCCTTCACACCTTTAGGTACCACGAGTGG CCACAGGGTCACTCATCCACAGACTATTCCCGCTGGCGGGAAGCCCAGGCACCATACAGTGTGCAGTGGT CAGCTGACTATGAACCCTACGTGGTGGTACCCCGTGACTGCCCCCGTTATGATCCTCGCTTTGTGGGATT TGGCTGGAACAAGGTGGCCCACATCATAGAGTTGGATGCTCAGGAATATGAATTCCTGGTACTTCCTGAG GCCTTCTCTATCCACTTGCCCCACGCTCCAAGTCTTGACATCTCCCGCTTCCGCTCCAGCCCCACCTACC GCAACTGTCTCCAGGCCCTCAAGGAAGAGTTCCACCAGGACTTGTCAAGGCGCTATGGGTCTGCAGCCCT GAAATACCTCACTGCCCTGCAGCAGGCCCGAAGTCGGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_172670 |
| Insert Size | 2073 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172670.3, NP_766258.2 |
| RefSeq Size | 2453 bp |
| RefSeq ORF | 2073 bp |
| Locus ID | 228366 |
| UniProt ID | Q5XPT3 |
| Gene Summary | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE (PubMed:15958417).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes isoform 1. CCDS Note: The coding region has been updated to represent an alternate exon combination and to extend the N-terminus to one that is more supported by available conservation data. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210059 | Gyltl1b (Myc-DDK-tagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 1 |
CNY 5,056.00 |
|
| MR210059L3 | Lenti ORF clone of Gyltl1b (Myc-DDK-tagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 1 |
CNY 7,980.00 |
|
| MR210059L4 | Lenti ORF clone of Gyltl1b (mGFP-tagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 1 |
CNY 7,980.00 |
