Rpgr (BC036959) Mouse Tagged ORF Clone

CAT#: MR209992

  • TrueORF®

Rpgr (Myc-DDK-tagged) - Mouse retinitis pigmentosa GTPase regulator (cDNA clone MGC:46892 IMAGE:4504793)

ORF Plasmid: DDK tGFP



  "BC036959" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,750.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209992 representing BC036959
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAAGAGGGTCGCGATGGGGGTCCCAGGGGGTAGGGCAGCACCTTAGGCTCAATCGAGTCGCCCCTG
CTATCTTTCCGAAGCAGGCACAGATTCCGTTCGCAGGCTTCGGCATGGCGGAATCTGAGTCACTGGTGCC
CGATACAGGTGCTGTGTTTACGTTTGGAAAAACTAAATTTGCCGAAAATATTCCTAGCAAATTCTGGTTT
AAAAATGACATACCCATATGTCTTTCATGTGGAGATGAACATACTGCTATTGTTACAGGAAATAATAAAT
TGTACATGTTCGGCAGTAACAACTGGGGTCAGTTAGGATTAGGATCAAAAGCTGCTATCATCAAGCCAAC
ATGTATCAAAGCTCTTAAGCCTGAGAAGGTGAAACTTGCTGCCTGTGGAAGGAACCACACCTTAGTTTCA
ACAGATACTGGTGGCGTATATGCAGCTGGTGGAAATAATGAAGGTCAACTGGGGCTTGGTGACACTGACG
ATAGAGACACCTTTCATCAAATTGTCTTCTTTACACCTGCTGATACCATTAAACAGCTCTCTGCTGGCGC
CAATACATCCGCTGCTCTTACTGAGGATGGAAAACTTTTTATGTGGGGTGACAATTCTGAAGGGCAGATT
GGTCTAGAAGATAAAAGTAATGTATGTATCCCTCATGAAGTGACTGTTGGAAAGCCAATTTCCTGGATCT
CTTGTGGATATTACCATTCAGCTTTTGTAACAATGGATGGGGAGCTCTACACATTTGGAGAACCCGAGAA
TGGGAAGTTGGGCCTTCCCAATGAGCTGCTGATGAATCACAGATCACCCCAGCGTGTGCTGGGCATTCCT
GAGAGGGTCATTCAAGTGGCCTGTGGTGGAGGGCACACTGTGGTTCTCACAGAGAAAGTTGTGTATGCCT
TTGGGCTGGGGCAGTTTGGACAACTGGGCCTTGGCACTTTTCTCTTTGAAACATCAGAACCCAAAATTAT
TGAGCGTATTAAGGATCAGAAAATATGTCATATTTCCTGTGGAGAAAACCATACAGCTTTGATGACAGAA
CTAGGCCTCCTGTATACTTTTGGAGACGGCCGACATGGAAAGTTAGGACTTGGGATGGAGAATTTCACCA
ATCAGTTCTTTCCTACCTTGTGCTCTAACTTTTTGAGATTTGCAGTTCAATTGATTGCCTGTGGTGGATG
TCATATGCTAGTTTTTGCCACTCCACGACTTGGTACAATAGATGAACCTAAATTTGAAGACGTATATGAG
CCTTATATAAGTACAGGTTCTTTTTCCATCAATGACCTCTCCCCAAGAAGTTCACTGAATAGATCTTTAT
CAGCACGTCTGCGGCGAAGAGAGCGGGAGAGACCCCCATGCTCAGCTTCAATGGTGGGAACACTGCCTCC
ATTAGAGGGGACTTCTGCCTCCACTTCAGCTTATTTTTACCCCAGTTCACCCCCCTTCCATTTGTCTGTG
AATAACTACCCAGAGAAAAGCCCCTCTGAATCAATGGAGCCACTGGACTCAGATTATTTTGAAGATAAAA
TGAACAAAGACACAGAGACAGAAAATTCTTCAGCAGTGGATTCAGAAAACTTTGGTGAAACTAATGATAT
CTTAAATATGACACATATGATGACTACGAGTTCCAATGAGAAGTTATTAGATTTTTCACCAATTCAAAAA
CAACAGGCTCCTCAACTTTCAGAAACTGTGAAACCAGAAGAAGGGGAAATGGATGAGGAAATTAGTATCC
TGAATGTAGAAGACACTGTAGAAGAAGAAAGGAAGGAAGGAGAGAAGGAGATTGTAGAAGAAGGAAGTAT
ACCTGAAACAGAAGGCTCAGAAACTATTGATATTACTGATGAGAAGCTAGATGAAGTCCTTAAAGAGGAA
GACAGTGCCAGCCTTCTGCAACGGGCCCTTCGTGAGTACAATGAAAATCCAAAAGGATCTGGAGACTCAC
CACTAGAAGACGATGGAGTCTGGTATTCACACTCTCTATATAAAGGTGAATTCTTTGCTTCTGTGCTGAC
AAGGACCCGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209992 representing BC036959
Red=Cloning site Green=Tags(s)

MPRGSRWGSQGVGQHLRLNRVAPAIFPKQAQIPFAGFGMAESESLVPDTGAVFTFGKTKFAENIPSKFWF
KNDIPICLSCGDEHTAIVTGNNKLYMFGSNNWGQLGLGSKAAIIKPTCIKALKPEKVKLAACGRNHTLVS
TDTGGVYAAGGNNEGQLGLGDTDDRDTFHQIVFFTPADTIKQLSAGANTSAALTEDGKLFMWGDNSEGQI
GLEDKSNVCIPHEVTVGKPISWISCGYYHSAFVTMDGELYTFGEPENGKLGLPNELLMNHRSPQRVLGIP
ERVIQVACGGGHTVVLTEKVVYAFGLGQFGQLGLGTFLFETSEPKIIERIKDQKICHISCGENHTALMTE
LGLLYTFGDGRHGKLGLGMENFTNQFFPTLCSNFLRFAVQLIACGGCHMLVFATPRLGTIDEPKFEDVYE
PYISTGSFSINDLSPRSSLNRSLSARLRRRERERPPCSASMVGTLPPLEGTSASTSAYFYPSSPPFHLSV
NNYPEKSPSESMEPLDSDYFEDKMNKDTETENSSAVDSENFGETNDILNMTHMMTTSSNEKLLDFSPIQK
QQAPQLSETVKPEEGEMDEEISILNVEDTVEEERKEGEKEIVEEGSIPETEGSETIDITDEKLDEVLKEE
DSASLLQRALREYNENPKGSGDSPLEDDGVWYSHSLYKGEFFASVLTRTR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC036959
ORF Size 2040 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC036959
RefSeq Size 2213 bp
RefSeq ORF 2042 bp
Locus ID 19893
MW 81.1 kDa
Gene Summary Could be a guanine-nucleotide releasing factor (By similarity). Plays a role in ciliogenesis (By similarity). Probably regulates cilia formation by regulating actin stress filaments and cell contractility (By similarity). May be involved in microtubule organization and regulation of transport in primary cilia (By similarity). Plays an important role in photoreceptor integrity. Isoform 5 may play a critical role in spermatogenesis and in intraflagellar transport processes.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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