Rpgr (BC036959) Mouse Tagged ORF Clone
CAT#: MG209992
- TrueORF®
Rpgr (tGFP-tagged) - Mouse retinitis pigmentosa GTPase regulator (cDNA clone MGC:46892 IMAGE:4504793)
ORF Plasmid: DDK
"BC036959" in other vectors (4)
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CNY 7,410.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG209992 representing BC036959
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAAGAGGGTCGCGATGGGGGTCCCAGGGGGTAGGGCAGCACCTTAGGCTCAATCGAGTCGCCCCTG CTATCTTTCCGAAGCAGGCACAGATTCCGTTCGCAGGCTTCGGCATGGCGGAATCTGAGTCACTGGTGCC CGATACAGGTGCTGTGTTTACGTTTGGAAAAACTAAATTTGCCGAAAATATTCCTAGCAAATTCTGGTTT AAAAATGACATACCCATATGTCTTTCATGTGGAGATGAACATACTGCTATTGTTACAGGAAATAATAAAT TGTACATGTTCGGCAGTAACAACTGGGGTCAGTTAGGATTAGGATCAAAAGCTGCTATCATCAAGCCAAC ATGTATCAAAGCTCTTAAGCCTGAGAAGGTGAAACTTGCTGCCTGTGGAAGGAACCACACCTTAGTTTCA ACAGATACTGGTGGCGTATATGCAGCTGGTGGAAATAATGAAGGTCAACTGGGGCTTGGTGACACTGACG ATAGAGACACCTTTCATCAAATTGTCTTCTTTACACCTGCTGATACCATTAAACAGCTCTCTGCTGGCGC CAATACATCCGCTGCTCTTACTGAGGATGGAAAACTTTTTATGTGGGGTGACAATTCTGAAGGGCAGATT GGTCTAGAAGATAAAAGTAATGTATGTATCCCTCATGAAGTGACTGTTGGAAAGCCAATTTCCTGGATCT CTTGTGGATATTACCATTCAGCTTTTGTAACAATGGATGGGGAGCTCTACACATTTGGAGAACCCGAGAA TGGGAAGTTGGGCCTTCCCAATGAGCTGCTGATGAATCACAGATCACCCCAGCGTGTGCTGGGCATTCCT GAGAGGGTCATTCAAGTGGCCTGTGGTGGAGGGCACACTGTGGTTCTCACAGAGAAAGTTGTGTATGCCT TTGGGCTGGGGCAGTTTGGACAACTGGGCCTTGGCACTTTTCTCTTTGAAACATCAGAACCCAAAATTAT TGAGCGTATTAAGGATCAGAAAATATGTCATATTTCCTGTGGAGAAAACCATACAGCTTTGATGACAGAA CTAGGCCTCCTGTATACTTTTGGAGACGGCCGACATGGAAAGTTAGGACTTGGGATGGAGAATTTCACCA ATCAGTTCTTTCCTACCTTGTGCTCTAACTTTTTGAGATTTGCAGTTCAATTGATTGCCTGTGGTGGATG TCATATGCTAGTTTTTGCCACTCCACGACTTGGTACAATAGATGAACCTAAATTTGAAGACGTATATGAG CCTTATATAAGTACAGGTTCTTTTTCCATCAATGACCTCTCCCCAAGAAGTTCACTGAATAGATCTTTAT CAGCACGTCTGCGGCGAAGAGAGCGGGAGAGACCCCCATGCTCAGCTTCAATGGTGGGAACACTGCCTCC ATTAGAGGGGACTTCTGCCTCCACTTCAGCTTATTTTTACCCCAGTTCACCCCCCTTCCATTTGTCTGTG AATAACTACCCAGAGAAAAGCCCCTCTGAATCAATGGAGCCACTGGACTCAGATTATTTTGAAGATAAAA TGAACAAAGACACAGAGACAGAAAATTCTTCAGCAGTGGATTCAGAAAACTTTGGTGAAACTAATGATAT CTTAAATATGACACATATGATGACTACGAGTTCCAATGAGAAGTTATTAGATTTTTCACCAATTCAAAAA CAACAGGCTCCTCAACTTTCAGAAACTGTGAAACCAGAAGAAGGGGAAATGGATGAGGAAATTAGTATCC TGAATGTAGAAGACACTGTAGAAGAAGAAAGGAAGGAAGGAGAGAAGGAGATTGTAGAAGAAGGAAGTAT ACCTGAAACAGAAGGCTCAGAAACTATTGATATTACTGATGAGAAGCTAGATGAAGTCCTTAAAGAGGAA GACAGTGCCAGCCTTCTGCAACGGGCCCTTCGTGAGTACAATGAAAATCCAAAAGGATCTGGAGACTCAC CACTAGAAGACGATGGAGTCTGGTATTCACACTCTCTATATAAAGGTGAATTCTTTGCTTCTGTGCTGAC AAGGACCCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209992 representing BC036959
Red=Cloning site Green=Tags(s) MPRGSRWGSQGVGQHLRLNRVAPAIFPKQAQIPFAGFGMAESESLVPDTGAVFTFGKTKFAENIPSKFWF KNDIPICLSCGDEHTAIVTGNNKLYMFGSNNWGQLGLGSKAAIIKPTCIKALKPEKVKLAACGRNHTLVS TDTGGVYAAGGNNEGQLGLGDTDDRDTFHQIVFFTPADTIKQLSAGANTSAALTEDGKLFMWGDNSEGQI GLEDKSNVCIPHEVTVGKPISWISCGYYHSAFVTMDGELYTFGEPENGKLGLPNELLMNHRSPQRVLGIP ERVIQVACGGGHTVVLTEKVVYAFGLGQFGQLGLGTFLFETSEPKIIERIKDQKICHISCGENHTALMTE LGLLYTFGDGRHGKLGLGMENFTNQFFPTLCSNFLRFAVQLIACGGCHMLVFATPRLGTIDEPKFEDVYE PYISTGSFSINDLSPRSSLNRSLSARLRRRERERPPCSASMVGTLPPLEGTSASTSAYFYPSSPPFHLSV NNYPEKSPSESMEPLDSDYFEDKMNKDTETENSSAVDSENFGETNDILNMTHMMTTSSNEKLLDFSPIQK QQAPQLSETVKPEEGEMDEEISILNVEDTVEEERKEGEKEIVEEGSIPETEGSETIDITDEKLDEVLKEE DSASLLQRALREYNENPKGSGDSPLEDDGVWYSHSLYKGEFFASVLTRTR TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC036959 |
ORF Size | 2042 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC036959 |
RefSeq Size | 2213 bp |
RefSeq ORF | 2042 bp |
Locus ID | 19893 |
Gene Summary | Could be a guanine-nucleotide releasing factor (By similarity). Plays a role in ciliogenesis (By similarity). Probably regulates cilia formation by regulating actin stress filaments and cell contractility (By similarity). May be involved in microtubule organization and regulation of transport in primary cilia (By similarity). Plays an important role in photoreceptor integrity. Isoform 5 may play a critical role in spermatogenesis and in intraflagellar transport processes.[UniProtKB/Swiss-Prot Function] |
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220753 | Rpgr (untagged) - Mouse retinitis pigmentosa GTPase regulator (cDNA clone MGC:46892 IMAGE:4504793), (10ug) |
CNY 9,120.00 |
|
MR209992 | Rpgr (Myc-DDK-tagged) - Mouse retinitis pigmentosa GTPase regulator (cDNA clone MGC:46892 IMAGE:4504793) |
CNY 6,750.00 |
|
MR209992L3 | Lenti ORF clone of Rpgr (Myc-DDK-tagged) - Mouse retinitis pigmentosa GTPase regulator (cDNA clone MGC:46892 IMAGE:4504793) |
CNY 8,650.00 |
|
MR209992L4 | Lenti ORF clone of Rpgr (mGFP-tagged) - Mouse retinitis pigmentosa GTPase regulator (cDNA clone MGC:46892 IMAGE:4504793) |
CNY 8,650.00 |