Ddx18 (NM_025860) Mouse Tagged ORF Clone
CAT#: MR209832
- TrueORF®
Ddx18 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (Ddx18)
ORF Plasmid: tGFP
"NM_025860" in other vectors (4)
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CNY 4,920.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2310005B10Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR209832 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCAGTTACAGATGAAACTCCTGCGCAGGAAGATCGAGAAGCGGAATGCCAAGCTGCGGCAGCGAA ACCTGAAGCTGCAAGAAACATCAGACACAAGCCTGTCCCAACCTCAAAATGGAGATGTGCCTAAAGAAAC AGGAAAAGGTGGAAAAGTGAAAAAGGCCCTGAAACGTTCTGTGCCTGTGGACTCAGCAGAAGCACAAAGT GGAGGCATGCCTGAGGAGACCTTGGAAAATGGCAAAGTTAAAAAGTCACCCCAGAAACTCACAACTTTAG CCAACGGAGAAGCAGCACCAACACCACCTCCTGATTCAGAAGTAAAAAAGAAAAAGAAGAAAAAGAGGAA AATGGCAAATGATGCCGGGCCTGATACAAAAAAAGCGAAAACTGAAGAAAGTGCTGAGGCTTGTGAGGAA CCAGAAGATGATGTGAAAAAGGCAGATGACAGCGAGGTGCCCAGCCTGCCCCTAGGACTGACAGGTGCTT TTGAGGACACTTCATTTGCTTCCTTGTCTAATCTCGTCAATGAGAACACCCTGAAGGCCATAGAAGAAAT GGGCTTCAAGCGCATGACGGAGATCCAGCATAAAAGTATCAGGCCACTTCTGGAAGGCAGGGACCTTCTG GCGGCTGCGAAAACCGGCAGTGGCAAAACCCTCGCTTTCCTCATCCCTGTGATCGAGCTCATTGTCAAGT TGAAGTTCATGCCCAGGAATGGAACAGGAGTCCTGATTCTCTCTCCTACCAGAGAACTGGCCATGCAGAC CTTTGGTGTTCTCAAGGAGCTGATGACGCACCACGTTCACACGTATGGGCTGATCATGGGCGGCAGCAAC AGGTCTGCCGAAGTGCAGAAGCTCCTCAATGGGATCAACATCATCGTGGCCACGCCCGGCCGGCTCCTGG ACCACATGCAGAACACGCCAGGGTTTATGTACAAGAACCTGCAGTGTCTGGTTATTGATGAGGCTGACCG TATCTTGGATGTTGGGTTTGAAGAAGAATTAAAGCAAATTATTAAACTTTTGCCAGCCCGTAGGCAAACC ATGCTCTTTTCTGCCACACAAACTCGAAAAGTTGAAGACTTGGCAAGAATTTCTCTGAAAAAGGAGCCTT TGTACGTGGGTGTTGATGACGACAAAGAGGTTGCCACAGTGGATGGCCTTGAGCAGGGCTATGTTGTTTG TCCCTCTGAGAAGAGGTTCCTTCTGCTTTTCACATTCCTTAAGAAGAACCGGAAGAAGAAAGTGATGGTC TTTTTTTCATCTTGTATGTCTGTGAAATACCACTATGAGCTGTTGAACTACATTGACCTTCCCGTCTTGG CCATCCATGGAAAGCAAAAGCAAAATAAGCGAACAACCACGTTTTTCCAGTTCTGCAACGCGGATTCAGG AATACTGCTGTGTACTGATGTGGCAGCCAGAGGGCTGGACATCCCCGAAGTGGACTGGATTGTTCAGTAC GACCCTCCCGATGACCCCAAGGAATACATTCATCGTGTTGGTAGAACAGCCAGGGGCCTGAATGGAAGAG GGCACGCCCTGCTCATCCTGCGCCCTGAAGAGTTGGGTTTCCTTCGTTACTTGAAACAGTCCAAGGTTCC ATTGAATCAGTTTGACTTCTCCTGGTCTAAAGTTTCTGATATCCAGTCTCAGCTGGAAAAACTGATCGAA AAGAACTACTTTCTCCACAAGTCTGCCCAGGAAGCATACAAATCCTACATAAGAGCATATGACTCTCACT CTCTCAAGCAGATCTTCAACGTGAACAACTTAAATCTGCCTCAGGTTGCTCTGTCTTTTGGTTTTAAGGT CCCTCCTTTTGTTGATCTGAATGTGAGCAGCCATGATGGCAAGCTTAAGAAGAGAGGCGGTGGTGGCGGA TTCGGCTACCAGAAAACAAAGAAAGTGGAGAAGTCCAAGATCTTTAAACACATCAGTAAGAAGCCAGCAG ACCGCAGGCAGTTCTCACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209832 protein sequence
Red=Cloning site Green=Tags(s) MSQLQMKLLRRKIEKRNAKLRQRNLKLQETSDTSLSQPQNGDVPKETGKGGKVKKALKRSVPVDSAEAQS GGMPEETLENGKVKKSPQKLTTLANGEAAPTPPPDSEVKKKKKKKRKMANDAGPDTKKAKTEESAEACEE PEDDVKKADDSEVPSLPLGLTGAFEDTSFASLSNLVNENTLKAIEEMGFKRMTEIQHKSIRPLLEGRDLL AAAKTGSGKTLAFLIPVIELIVKLKFMPRNGTGVLILSPTRELAMQTFGVLKELMTHHVHTYGLIMGGSN RSAEVQKLLNGINIIVATPGRLLDHMQNTPGFMYKNLQCLVIDEADRILDVGFEEELKQIIKLLPARRQT MLFSATQTRKVEDLARISLKKEPLYVGVDDDKEVATVDGLEQGYVVCPSEKRFLLLFTFLKKNRKKKVMV FFSSCMSVKYHYELLNYIDLPVLAIHGKQKQNKRTTTFFQFCNADSGILLCTDVAARGLDIPEVDWIVQY DPPDDPKEYIHRVGRTARGLNGRGHALLILRPEELGFLRYLKQSKVPLNQFDFSWSKVSDIQSQLEKLIE KNYFLHKSAQEAYKSYIRAYDSHSLKQIFNVNNLNLPQVALSFGFKVPPFVDLNVSSHDGKLKKRGGGGG FGYQKTKKVEKSKIFKHISKKPADRRQFSH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025860 |
ORF Size | 1983 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025860.2, NP_080136.2 |
RefSeq Size | 2249 bp |
RefSeq ORF | 1983 bp |
Locus ID | 66942 |
UniProt ID | Q8K363 |
MW | 74.2 kDa |
Gene Summary | Probable RNA-dependent helicase.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202238 | Ddx18 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (Ddx18), (10ug) |
CNY 4,920.00 |
|
MG209832 | Ddx18 (tGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (Ddx18) |
CNY 5,040.00 |
|
MR209832L3 | Lenti ORF clone of Ddx18 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (Ddx18) |
CNY 6,460.00 |
|
MR209832L4 | Lenti ORF clone of Ddx18 (mGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (Ddx18) |
CNY 6,460.00 |