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Tie1 (BC060182) Mouse Tagged ORF Clone
CAT＃: MR209734
- TrueORF®
Tie1 (Myc-DDK-tagged) - Mouse tyrosine kinase receptor 1 (cDNA clone MGC:63223 IMAGE:6831929)
ORF Plasmid: tGFP
"BC060182" in other vectors (4)
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CNY 6,460.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D430008P04Rik; TIE; tie-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209734 representing BC060182
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTGGTGGGGATCCTCTTTGCTGCTCCCCACTCTTTTCTTGGCCTCTCATGTTGGTGCGTCTGTTG ACTTAACATTGCTGGCCAACCTGCGCATCACAGACCCCCAGCGTTTCTTCCTGACCTGCGTGTCTGGTGA GGCCGGAGCAGGGAGGAGCTCGGACCCGCCCCTGCTGCTGGAGAAGGATGATCGCATCGTGCGCACCTTC CCGCCTGGGCAGCCCCTGTACCTGGCACGCAACGGTTCTCACCAGGTCACGCTGAGGGGCTTCTCCAAGC CCTCGGATTTGGTAGGCGTCTTCTCCTGTGTGGGTGGTGCTGGCGCGCGGCGCACTCGAGTTCTCTATGT GCACAACAGCCCAGGGGCACACCTGTTTCCAGACAAGGTCACACACACGGTGAACAAAGGTGACACTGCT GTGCTTTCTGCCCATGTGCACAAGGAAAAGCAGACTGACGTGATCTGGAAGAACAACGGATCCTACTTCA ATACCCTAGACTGGCAAGAGGCCGATGACGGGCGTTTTCAACTGCAGCTCCAAAATGTACAGCCACCATC AAGTGGCATCTACAGTGCCACCTACCTAGAGGCCAGCCCCCTGGGCAGTGCCTTCTTTCGGCTCATCGTG CGAGGCTGTGGGGCTGGACGCTGGGGGCCAGGATGTGTCAAGGATTGCCCAGGCTGCCTGCATGGAGGTG TCTGTCATGACCATGATGGCGAATGTGTGTGTCCCCCAGGATTCACTGGCACCCGCTGTGAGCAGGCCTG CAGGGAGGGTCGTTTTGGACAGAGCTGTCAGGAACAGTGCCCAGGCACAGCAGGTTGTAGAGGTCTCACC TTCTGCCTCCCGGATCCCTATGGCTGTTCCTGTGGATCTGGCTGGAGAGGAAGCCAGTGCCAGGAAGCAT GTGCACCTGGTCATTTTGGGGCTGATTGTCGCCTCCAGTGCCAGTGTCAAAATGGTGGCACTTGTGACCG GTTCAGCGGCTGCGTCTGCCCCTCTGGGTGGCATGGAGTCCACTGTGAAAAGTCAGACCGGATCCCCCAG ATCCTCAGTATGGCCACAGAGGTGGAGTTCAACATAGGGACGATGCCCCGGATCAACTGTGCAGCTGCCG GGAATCCCTTCCCAGTGCGGGGCAGCATGAAACTTCGCAAGCCAGATGGCACCATGCTTTTGTCTACCAA AGTCATTGTGGAGCCAGACAGGACCACAGCAGAGTTTGAAGTGCCCAGTTTGACTCTTGGGGACAGTGGG TTCTGGGAATGCCGCGTATCGACTTCTGGTGGCCAAGACAGCCGGCGCTTCAAGGTCAATGTCAAAGTAC CCCCAGTGCCTTTGACTGCACCTCGACTCCTGGCCAAGCAGAGCCGTCAGCTTGTGGTCTCCCCACTGGT CTCCTTTAGTGGGGATGGACCCATCTCCTCTGTCCGCCTGCACTACCGGCCCCAGGACAGCACGATTGCC TGGTCTGCCATTGTGGTGGATCCCAGTGAGAATGTGACATTAATGAACCTGAAGCCAAAGACAGGATACA ATGTCCGAGTGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAAGGAGGCTGGGGACCTTCTGCCCTTATGAC TACTGACTGTCCTGAGCCTTTGCTGCAGCCCTGGCTAGAGAGCTGGCACGTGGAGGGTCCTGACAGGCTC CGTGTGAGCTGGTCTCTACCCTCGGTGCCACTGTCCGGTGATGGTTTCCTGCTGCGCCTGTGGGATGGGG CCCGGGGACAGGAGAGGCGGGAGAACATCTCATTCCCCCAGGCCCGCACTGCCCTCCTGACTGGACTCAC GCCGGGCACCCACTACCAGCTGGATGTGCGACTGTATCACTGTACCCTCCTGGGCCCTGCCTCACCCCCT GCGCATGTGCACCTGCCCCCCAGCGGTAGGCCCACTCTGGGAAGGATGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209734 representing BC060182
Red=Cloning site Green=Tags(s) MVWWGSSLLLPTLFLASHVGASVDLTLLANLRITDPQRFFLTCVSGEAGAGRSSDPPLLLEKDDRIVRTF PPGQPLYLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVLYVHNSPGAHLFPDKVTHTVNKGDTA VLSAHVHKEKQTDVIWKNNGSYFNTLDWQEADDGRFQLQLQNVQPPSSGIYSATYLEASPLGSAFFRLIV RGCGAGRWGPGCVKDCPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGTAGCRGLT FCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQ ILSMATEVEFNIGTMPRINCAAAGNPFPVRGSMKLRKPDGTMLLSTKVIVEPDRTTAEFEVPSLTLGDSG FWECRVSTSGGQDSRRFKVNVKVPPVPLTAPRLLAKQSRQLVVSPLVSFSGDGPISSVRLHYRPQDSTIA WSAIVVDPSENVTLMNLKPKTGYNVRVQLSRPGEGGEGGWGPSALMTTDCPEPLLQPWLESWHVEGPDRL RVSWSLPSVPLSGDGFLLRLWDGARGQERRENISFPQARTALLTGLTPGTHYQLDVRLYHCTLLGPASPP AHVHLPPSGRPTLGRMG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC060182 |
| ORF Size | 1941 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060182.1 |
| RefSeq Size | 4259 bp |
| RefSeq ORF | 1943 bp |
| Locus ID | 21846 |
| MW | 156.1 kDa |
| Gene Summary | Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218258 | Tie1 (untagged) - Mouse tyrosine kinase receptor 1 (cDNA clone MGC:63223 IMAGE:6831929), (10ug) |
CNY 6,940.00 |
|
| MG209734 | Tie1 (tGFP-tagged) - Mouse tyrosine kinase receptor 1 (cDNA clone MGC:63223 IMAGE:6831929) |
CNY 9,304.00 |
|
| MR209734L3 | Lenti ORF clone of Tie1 (Myc-DDK-tagged) - Mouse tyrosine kinase receptor 1 (cDNA clone MGC:63223 IMAGE:6831929) |
CNY 8,360.00 |
|
| MR209734L4 | Lenti ORF clone of Tie1 (mGFP-tagged) - Mouse tyrosine kinase receptor 1 (cDNA clone MGC:63223 IMAGE:6831929) |
CNY 8,360.00 |
