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Slc27a1 (NM_011977) Mouse Tagged ORF Clone
CAT＃: MR209726
- TrueORF®
Slc27a1 (Myc-DDK-tagged) - Mouse solute carrier family 27 (fatty acid transporter), member 1 (Slc27a1)
ORF Plasmid: tGFP
"NM_011977" in other vectors (4)
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CNY 7,224.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fatp; FATP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209726 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCTCCTGGAGCAGGAACAGCCTCTGTGGCCTCACTGGCGCTGCTTTGGTTTCTGGGACTTCCGT GGACCTGGAGCGCGGCGGCGGCGTTCTGTGTGTACGTGGGTGGCGGCGGCTGGCGCTTTCTGCGTATCGT CTGCAAGACGGCGAGGCGAGACCTCTTTGGCCTCTCTGTTCTGATTCGTGTTCGGCTAGAGCTGCGACGA CACCGGCGAGCAGGAGACACGATCCCGTGCATCTTCCAGGCTGTGGCCCGGCGACAACCAGAGCGCCTGG CACTGGTGGACGCCAGTAGTGGTATATGCTGGACCTTCGCACAGCTGGACACCTACTCCAATGCTGTAGC CAACCTGTTCCGCCAGCTGGGCTTTGCACCAGGCGATGTGGTGGCTGTGTTCCTGGAGGGCCGGCCGGAG TTCGTGGGACTGTGGCTGGGCCTGGCCAAGGCCGGTGTGGTGGCTGCTCTTCTCAATGTCAACCTGAGGC GGGAGCCCCTGGCCTTCTGCCTGGGCACATCAGCTGCCAAGGCCCTCATTTATGGCGGGGAGATGGCAGC GGCGGTGGCGGAGGTGAGCGAGCAGCTGGGGAAGAGCCTCCTCAAGTTCTGCTCTGGAGATCTGGGGCCT GAGAGCATCCTGCCTGACACGCAGCTCCTGGACCCCATGCTTGCTGAGGCGCCCACCACACCCCTGGCAC AAGCCCCAGGCAAGGGCATGGATGATCGGCTGTTTTACATCTATACTTCTGGGACCACCGGGCTTCCTAA GGCTGCCATTGTGGTGCACAGCAGGTACTACCGCATTGCTGCCTTTGGCCACCATTCCTACAGCATGCGT GCCGCCGATGTGCTCTATGACTGCCTGCCACTCTACCACTCTGCAGGGAACATCATGGGTGTGGGGCAGT GCGTCATCTACGGGTTGACGGTGGTACTGCGCAAGAAGTTCTCCGCCAGCCGCTTCTGGGATGACTGTGT CAAGTACAATTGCACGGTAGTGCAGTACATAGGTGAAATCTGCCGCTACCTGCTGAGGCAGCCGGTTCGC GACGTGGAGCAGCGACACCGCGTGCGCCTGGCCGTGGGTAATGGGCTGCGGCCAGCCATCTGGGAGGAGT TCACGCAGCGCTTCGGTGTGCCACAGATCGGCGAGTTCTACGGCGCTACCGAGTGCAACTGCAGCATTGC CAACATGGACGGCAAGGTCGGCTCCTGCGGCTTCAACAGCCGTATCCTCACGCATGTGTACCCCATCCGT CTGGTCAAGGTCAATGAGGACACGATGGAGCCACTGCGGGACTCCGAGGGCCTCTGCATCCCGTGCCAGC CCGGGGAACCCGGCCTTCTCGTGGGCCAGATCAACCAGCAGGACCCTCTGCGGCGTTTCGATGGTTATGT TAGTGACAGTGCCACCAACAAGAAGATTGCCCACAGCGTTTTCCGAAAGGGCGATAGCGCCTACCTCTCA GGTGACGTGCTAGTGATGGACGAGCTGGGCTACATGTATTTCCGTGACCGCAGCGGGGACACCTTCCGCT GGCGCGGGGAGAACGTGTCCACCACGGAGGTGGAAGCCGTGCTGAGCCGCCTACTGGGCCAGACGGACGT GGCTGTGTATGGGGTGGCTGTGCCAGGAGTGGAGGGGAAAGCTGGCATGGCAGCCATCGCAGATCCCCAC AGCCAGTTGGACCCTAACTCAATGTACCAGGAATTACAGAAGGTTCTTGCATCCTATGCTCGGCCCATCT TCCTGCGTCTTCTGCCCCAGGTGGATACCACAGGCACCTTCAAGATCCAGAAGACCCGGCTGCAGCGTGA AGGCTTTGACCCCCGTCAGACCTCAGACAGGCTCTTCTTTCTAGACCTGAAGCAGGGACGCTATGTACCC CTGGATGAGAGAGTCCATGCCCGCATTTGTGCAGGCGACTTCTCACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209726 protein sequence
Red=Cloning site Green=Tags(s) MRAPGAGTASVASLALLWFLGLPWTWSAAAAFCVYVGGGGWRFLRIVCKTARRDLFGLSVLIRVRLELRR HRRAGDTIPCIFQAVARRQPERLALVDASSGICWTFAQLDTYSNAVANLFRQLGFAPGDVVAVFLEGRPE FVGLWLGLAKAGVVAALLNVNLRREPLAFCLGTSAAKALIYGGEMAAAVAEVSEQLGKSLLKFCSGDLGP ESILPDTQLLDPMLAEAPTTPLAQAPGKGMDDRLFYIYTSGTTGLPKAAIVVHSRYYRIAAFGHHSYSMR AADVLYDCLPLYHSAGNIMGVGQCVIYGLTVVLRKKFSASRFWDDCVKYNCTVVQYIGEICRYLLRQPVR DVEQRHRVRLAVGNGLRPAIWEEFTQRFGVPQIGEFYGATECNCSIANMDGKVGSCGFNSRILTHVYPIR LVKVNEDTMEPLRDSEGLCIPCQPGEPGLLVGQINQQDPLRRFDGYVSDSATNKKIAHSVFRKGDSAYLS GDVLVMDELGYMYFRDRSGDTFRWRGENVSTTEVEAVLSRLLGQTDVAVYGVAVPGVEGKAGMAAIADPH SQLDPNSMYQELQKVLASYARPIFLRLLPQVDTTGTFKIQKTRLQREGFDPRQTSDRLFFLDLKQGRYVP LDERVHARICAGDFSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011977 |
| ORF Size | 1941 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011977.4 |
| RefSeq Size | 2795 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 26457 |
| UniProt ID | Q60714 |
| MW | 71.3 kDa |
| Gene Summary | Mediates the ATP-dependent import of long-chain fatty acids (LCFA) into the cell by mediating their translocation at the plasma membrane (PubMed:7954810, PubMed:9786857, PubMed:9671728, PubMed:10471110, PubMed:12235169, PubMed:11970897, PubMed:15699031, PubMed:28178239). Has also an acyl-CoA ligase activity for long-chain and very-long-chain fatty acids (PubMed:10593920, PubMed:12235169, PubMed:12937175). May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation (PubMed:14991074, PubMed:15897321). Plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis (PubMed:12235169). May be involved in regulation of cholesterol metabolism (PubMed:12235169).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201650 | Slc27a1 (untagged) - Mouse solute carrier family 27 (fatty acid transporter), member 1 (Slc27a1), (10ug) |
CNY 7,224.00 |
|
| MG209726 | Slc27a1 (tGFP-tagged) - Mouse solute carrier family 27 (fatty acid transporter), member 1 (Slc27a1) |
CNY 8,824.00 |
|
| MR209726L3 | Lenti ORF clone of Slc27a1 (Myc-DDK-tagged) - Mouse solute carrier family 27 (fatty acid transporter), member 1 (Slc27a1) |
CNY 8,360.00 |
|
| MR209726L4 | Lenti ORF clone of Slc27a1 (mGFP-tagged) - Mouse solute carrier family 27 (fatty acid transporter), member 1 (Slc27a1) |
CNY 8,360.00 |
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