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Racgap1 (BC010715) Mouse Tagged ORF Clone
CAT＃: MR209580
- TrueORF®
Racgap1 (Myc-DDK-tagged) - Mouse Rac GTPase-activating protein 1 (cDNA clone MGC:11396 IMAGE:3602242)
ORF Plasmid: tGFP
"BC010715" in other vectors (4)
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CNY 4,680.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Band25, GTPase, MgcRacGAP, gtl11 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209580 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATACTACAATGGTGAATTTGTGGACTCTGTTTGAGCAGCTTGTGCGCCGGATGGAGATTATCAATG AAGGAAATGAAAGCATTGAATTCATCCAGGTTGTGAAGGACTTCGAGGACTTCCGAAAGAAGTATCAAAG AACCAACCAGGAGCTGGAGAAATTCAAAGACCTATTGTTGAAAGCAGAGACGGGGCGGAGCGCCCTGGAC GTGAAGCTGAAGCATGCCCGTAATCAAGTGGACGTGGAGATCAAGCGGAGGCAGCGCGCTGAGGCAGAGT GTGCAAAGCTGGAACAACAGATTCAGCTGATTCGAGACATACTCATGTGTGACACATCTGGCAGTATTCA GCTGAGCGAGGAACAAAAATCAGCTCTCGCTTTCCTCAACCGAGGCCAAGCATCCAGTGGCCACGCCGGC AACAATAGACTGTCAACGATTGATGAATCTGGTTCCATTTTATCAGATATCAGCTTTGACAAGACTGATG AATCACTGGACTGGGATTCTTCTTTGGTGAAGAATTTCAAAATGAAGAAACGAGAGAAGAGGCGCTCCAA CAGTAGACAGTTCATCGACGGCCCTCCTGGGCCTGTGAAGAAAACTTGTTCCATTGGCTCTACAGTAGAC CAGGCAAATGAATCAATAGTTGCAAAAACTACAGTGACTGTTCCCAGTGATGGGGGACCCATTGAAGCTG TGTCTACTATTGAGACATTGCCGTCCTGGACCAGGAGTCGCGGGAAGTCAGGACCTTTACAACCTGTGAA CAGTGACTCCGCTCTGAACAGCAGGCCACTGGAGCCAAGAACTGACACAGACAATTTGGGCACACCTCAG AATACTGGAGGCATGCGCTTGCACGACTTCGTCTCAAAGACGGTTATTAAGCCTGAATCTTGTGTTCCGT GTGGAAAGCGGATCAAGTTTGGCAAGCTGTCTCTGAAGTGTCGAGACTGTCGTTTGGTCTCCCATCCAGA ATGTCGGGACCGATGTCCCCTTCCCTGCATCCCCCCCCTGGTGGGGACACCGGTTAAGATTGGAGAGGGC ATGCTGGCCGACTTCGTGTCGCAGGCTTCTCCCATGATCCCTGCCATTGTCGTCAGCTGTGTCAATGAGA TCGAGCAGCGAGGCCTGACTGAGGCAGGCTTGTACAGGATCTCAGGCTGTGACCGCACAGTGAAAGAACT GAAAGAAAAATTCCTTAAGGTGAAAACTGTGCCCCTCCTCAGCAAAGTGGACGATATCCACGTCATCTGC AGCCTCCTGAAGGACTTCCTGCGCAACCTCAAAGAGCCCCTCCTGACCTTCTGGCTGAGCAAAGCCTTCA TGGAGGCAGCAGAGATAACAGATGAAGACAACAGCACAGCCGCCATGTACCAGGCTGTCAGTGAGCTGCC CCAGGCCAACAGGGACACGCTAGCCTTCCTTATGATCCACCTACAGAGAGTGTCTCAGAGTCCAGACACT AAGATGGATATTGCCAATCTAGCTAAAGTCTTTGGCCCTACAATAGTTGCCCATACTGTGCCCAATCCAG ATCCAGTGACAATGTTCCAGGACATCAAACGTCAGCTCAAGGTGGTGGAGCGCCTACTCTCTCTCCCCTT GGAGTACTGGAATCAGTTCATGATGGTGGACCAAGAGAACATAGACAGCCAGCGAGGCAATGGAAACTCA ACACCACGCACACCAGACGTTAAAGTGAGCTTACTGGGGCCTGTGACCACTCCTGAATTCCAGCTTGTCA AGACTCCTTTATCAAGTTCCCTGTCACAGAGGTTGTACAACCTCTCCAAGAGCACACCCAGATTTGGGAA CAAGAGCAAGTCTGCCACCAACTTAGGTCAACAGGGCAAATTTTTCCCTGCTCCGTACCTCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209580 protein sequence
Red=Cloning site Green=Tags(s) MDTTMVNLWTLFEQLVRRMEIINEGNESIEFIQVVKDFEDFRKKYQRTNQELEKFKDLLLKAETGRSALD VKLKHARNQVDVEIKRRQRAEAECAKLEQQIQLIRDILMCDTSGSIQLSEEQKSALAFLNRGQASSGHAG NNRLSTIDESGSILSDISFDKTDESLDWDSSLVKNFKMKKREKRRSNSRQFIDGPPGPVKKTCSIGSTVD QANESIVAKTTVTVPSDGGPIEAVSTIETLPSWTRSRGKSGPLQPVNSDSALNSRPLEPRTDTDNLGTPQ NTGGMRLHDFVSKTVIKPESCVPCGKRIKFGKLSLKCRDCRLVSHPECRDRCPLPCIPPLVGTPVKIGEG MLADFVSQASPMIPAIVVSCVNEIEQRGLTEAGLYRISGCDRTVKELKEKFLKVKTVPLLSKVDDIHVIC SLLKDFLRNLKEPLLTFWLSKAFMEAAEITDEDNSTAAMYQAVSELPQANRDTLAFLMIHLQRVSQSPDT KMDIANLAKVFGPTIVAHTVPNPDPVTMFQDIKRQLKVVERLLSLPLEYWNQFMMVDQENIDSQRGNGNS TPRTPDVKVSLLGPVTTPEFQLVKTPLSSSLSQRLYNLSKSTPRFGNKSKSATNLGQQGKFFPAPYLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC010715 |
| ORF Size | 1884 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC010715, AAH10715 |
| RefSeq Size | 2913 bp |
| RefSeq ORF | 1886 bp |
| Locus ID | 26934 |
| MW | 70.2 kDa |
| Gene Summary | Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Induction of MNK kinases-dependent eIF4E phosphorylation by inhibitors targeting BET proteins limits efficacy of BET inhibitors
,Pham, TN;Kumar, K;DeCant, BT;Shang, M;Munshi, SZ;Matsangou, M;Ebine, K;Munshi, HG;,
Mol. Cancer Ther.
,PubMed ID 30446586
[RACGAP1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200700 | Racgap1 (untagged) - Mouse Rac GTPase-activating protein 1 (cDNA clone MGC:11396 IMAGE:3602242), (10ug) |
CNY 4,680.00 |
|
| MG209580 | Racgap1 (tGFP-tagged) - Mouse Rac GTPase-activating protein 1 (cDNA clone MGC:11396 IMAGE:3602242) |
CNY 3,990.00 |
|
| MR209580L3 | Lenti ORF clone of Racgap1 (Myc-DDK-tagged) - Mouse Rac GTPase-activating protein 1 (cDNA clone MGC:11396 IMAGE:3602242) |
CNY 5,510.00 |
|
| MR209580L4 | Lenti ORF clone of Racgap1 (mGFP-tagged) - Mouse Rac GTPase-activating protein 1 (cDNA clone MGC:11396 IMAGE:3602242) |
CNY 5,510.00 |
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