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Rbm14 (BC010294) Mouse Tagged ORF Clone
CAT＃: MR209471
- TrueORF®
Rbm14 (Myc-DDK-tagged) - Mouse RNA binding motif protein 14 (cDNA clone MGC:11556 IMAGE:3153900)
ORF Plasmid: tGFP
"BC010294" in other vectors (4)
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CN¥ 4,600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | p16K, Sytip, p16 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209471 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATTTTTGTGGGCAATGTCGATGGGGCGGATACGACACCGGAGGAGTTGGCAGCTCTCTTCGCGC CCTATGGCACGGTCATGAGCTGCGCCGTCATGAAACAGTTTGCCTTCGTGCACATGCGCGAGAACGCTGG CGCGGTGCGCGCCATCGAGGCCCTGCATGGCCACGAGCTGCGTCCAGGTCGCGCGCTCGTGGTGGAGATG TCGCGCCCGAGGCCCCTGAACACTTGGAAGATTTTCGTGGGCAATGTATCGGCTGCATGTACAAGTCAGG AATTGCGCAGCCTCTTCGAGCGCCGTGGACGCGTCATCGAGTGTGACGTGGTAAAAGACTACGCGTTTGT TCACATGGAGAAGGAAGCAGATGCCAAAGCCGCCATCGCGCAGCTCAACGGCAAAGAAGTGAAGGGCAAG CGCATCAACGTGGAACTCTCAACCAAGGGGCAGAAGAAGGGGCCTGCCCTGGCTATCCAGTCTGGGGACA AGACCAAGAAACCAGGGGCTGGGGACACAGCATTCCCTGGAACTGGTGGCTTCTCTGCCACCTTCGACTA CCAGCAGGCTTTTGGCAACAGCACTGGTGGCTTTGATGGGCAAGCCCGTCAGCCCACACCACCATTCTTT GGTCGCGACCGCAGCCCCCTGCGCCGTTCACCTCCTCGAGCCTCTTATGTGGCGCCTCTGACGGCCCAGC CAGCCACCTACCGGGCCCAACCCTCAGTGTCTCTGGGAGCTGCATATAGGGCCCAGCCTTCCGCCTCTTT GGGTGTCGGCTATCGGACTCAGCCCATGGCAGCTCAGGCAGCTTCTTACCGTGCTCAGCCCTCTGTCTCC CTTGGGGCCCCATACAGGGGTCAGCTGGCTAGCCCTAGTTCCCAGTCTGCTGCAGCTTCCTCGCTTGGCC CATATGGTGGAGTCCAGCCCTCAGCCTCAGCCCTCTCCACTTATGGGGGTCAGGCAGCTGCAGCTTCTTC GCTTAATTCCTATGGGGCTCAGGGCTCCTCCCTTGCTTCCTATGGTAACCAGCCATCCTCTTATGGCGCC CAGGCTGCCTCTTCGTATGGGGTTCGTGCGGCTGCTTCTTCCTATAACACACAAGGAGCAGCTTCTTCCC TAGGTTCCTATGGGGCTCAAGCAGCCTCCTATGGTGCCCAGTCTGCAGCCTCCTCACTAGCTTATGGAGC CCAGGCAGCGTCTTACAGTGCCCAGCCCTCAGCCTCTTACAGTGCCCAGTCTGCCCCATATGCTGCACAA CAGGCTGCTTCTTATTCTTCCCAGCCTGCTGCTTATGTGGCACAACCAGCCACAGCTGCTGCCTATGCTA GCCAGCCAGCTGCCTATGCTGCACAAGCCACTACCCCAATGGCTGGTTCCTATGGGGCTCAACCAGTTGT TCAGACCCAGCTGAATAGCTATGGGGCTCAAGCATCTATTGGCCTGTCAGGCTCATATGGAGCTCAGTCT GCTGCTGCGGCCACTGGCTCCTATGGGGCGGCAGCTGCTTACGGGGCTCAGCCTTCTGCCACCCTGGCAG CTCCTTACCGCACTCAGTCATCAGCCTCATTGGCTGCTTCCTATGCTGCCCAGCAGCATCCCCAGGCTGC TGCCTCCTACCGTGGCCAGCCGGGCAGTGCCTACGATGGGACAGGCCAGCCATCAGCAGCCTACCTGTCT ATGTCCCAGGGGGCCGTTGCCAACGCCAACAGCACCCCGCCGCCCTATGAGCGTACCCGCCTCTCCCCAC CCCGGGCCAGCTACGACGATCCCTACAAAAAGGCTGTCGCCATGTCAAAAAGGTACTGTATGCCCCCCCG CCTCAGCCCCCAGCTGGGGCTTAGGGCAAGGGGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR209471 protein sequence
Red=Cloning site Green=Tags(s) MKIFVGNVDGADTTPEELAALFAPYGTVMSCAVMKQFAFVHMRENAGAVRAIEALHGHELRPGRALVVEM SRPRPLNTWKIFVGNVSAACTSQELRSLFERRGRVIECDVVKDYAFVHMEKEADAKAAIAQLNGKEVKGK RINVELSTKGQKKGPALAIQSGDKTKKPGAGDTAFPGTGGFSATFDYQQAFGNSTGGFDGQARQPTPPFF GRDRSPLRRSPPRASYVAPLTAQPATYRAQPSVSLGAAYRAQPSASLGVGYRTQPMAAQAASYRAQPSVS LGAPYRGQLASPSSQSAAASSLGPYGGVQPSASALSTYGGQAAAASSLNSYGAQGSSLASYGNQPSSYGA QAASSYGVRAAASSYNTQGAASSLGSYGAQAASYGAQSAASSLAYGAQAASYSAQPSASYSAQSAPYAAQ QAASYSSQPAAYVAQPATAAAYASQPAAYAAQATTPMAGSYGAQPVVQTQLNSYGAQASIGLSGSYGAQS AAAATGSYGAAAAYGAQPSATLAAPYRTQSSASLAASYAAQQHPQAAASYRGQPGSAYDGTGQPSAAYLS MSQGAVANANSTPPPYERTRLSPPRASYDDPYKKAVAMSKRYCMPPRLSPQLGLRARG SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC010294 |
| ORF Size | 1854 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC010294, AAH10294 |
| RefSeq Size | 3545 bp |
| RefSeq ORF | 1856 bp |
| Locus ID | 56275 |
| MW | 63.2 kDa |
| Gene Summary | May function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1 (By similarity). Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity (PubMed:25385835). Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ (By similarity). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200669 | Rbm14 (untagged) - Mouse RNA binding motif protein 14 (cDNA clone MGC:11556 IMAGE:3153900), (10ug) |
CN¥ 4,608.00 |
|
| MG209471 | Rbm14 (tGFP-tagged) - Mouse RNA binding motif protein 14 (cDNA clone MGC:11556 IMAGE:3153900) |
CN¥ 3,900.00 |
|
| MR209471L3 | Lenti ORF clone of Rbm14 (Myc-DDK-tagged) - Mouse RNA binding motif protein 14 (cDNA clone MGC:11556 IMAGE:3153900) |
CN¥ 5,420.00 |
|
| MR209471L4 | Lenti ORF clone of Rbm14 (mGFP-tagged) - Mouse RNA binding motif protein 14 (cDNA clone MGC:11556 IMAGE:3153900) |
CN¥ 5,420.00 |
