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Ankrd25 (BC010245) Mouse Tagged ORF Clone
CAT＃: MR209360
- TrueORF®
Ankrd25 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 25 (cDNA clone MGC:12143 IMAGE:3710765)
ORF Plasmid: tGFP
"BC010245" in other vectors (4)
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CNY 6,080.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC12143, MGC7734 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209360 representing BC010245
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGGTCCTGCATGTGCCCGCCCCATTCCCAGGAACCCCTGGTCAGGCCTCCCCAGCGGCCTTCC CCAGCAAGGAGCCAGACCCACCGTACTCAGTGGAAACGCCCTATGGCTACCGCCTGGACCTGGATTTTCT TAAGTACGTGGATGACATTGAGAAGGGCCACACACTGCGCAGGGTGGCGGTACAGCGCCGGCCGCGCCTG GGTTCCCTGCCACGGGGCCCGGGCTCCTGGTGGACGTCCACAGAGTCTCTGTGCTCTGATGCCAGTGGGG ACAGCCGGCACTCGGCCTACTCCTACTGTGGCCGCGGCTTCTACCCACAGTACGGAGCCTTGGAGACCCG AATCGGCTCCAACCCTCGTGTGGAGCGCACGCTGCTGGATGCGCGGCGCCGGCTGGAGGACCAGGTGGCT GCCCCATCGTCCGGTGGCCTGGGCTCCCTCACACCCAGCGCCGCGGGCTCCACCAGCTCCCTGGCAGGCG TGGGCCTGCTGCCCCCCACGCCTCGCAGCTCCGGCCTGTCCACCCCGGTGGCACCCAGCGCTGGTCACCT AGCCCACGTGCGCGAGCAGATGGCTGGAGCCCTGCGCAAGCTGCGCCAGCTGGAGGAGCAGGTCAAGCTG ATCCCCGTTCTCCAAGTCAAGCTGTCAGTGCTCCAGGAGGAAAAGAGACAGCTCACCGTGCAGCTCAAGA GCCAGAAGTTTCTAGGTCACCCCTCAGGGACACGGAGCCGGAGTGAGCTCTGCCTGGACCTTCCCGAGGC CCCTGACGACCCTGCTGCACTAGAGACCCGCTCGGTGGGCACCTGGGTTAGAGAACGGGACTTGGGCATT CCAGATGGTGAAGCTGCCCTGGTAGCTAAAGTGGCCGTGTTGGAGACCCAGCTCAAAAAAGCGCTACAGG AGCTGCGGGCGGCTCAGACCCAGCAGGTCGACCTCCAGCCCCAGGCCTGGCCACCACCAGACACCCAGGT CCGCGTGGACACCGTGCGAGTAGTGGAGGGTCCCCGGGAAGTGGAGGTGGCAGCCAGCACGGCAGCCGGG GCCCTTGCACAGCGAGCTCAGAGTCTGGAACCCTACGGAACAGGGCTGAAGGCCCTGACCACATCTGGGG GACCAGAGAACACCCTCGTGTTCCGCAGTCATGAAGTGGTGGAGACCATGTGCCCACTGCCCACAGCTAC CACTGGCAATGTGCACACAGCCAAGAAGATCAGCATTACAGAACGGAGTTGCACCGGTGCACCCAGGATG ACAGAGCCTTCCTCCGTTAATCCACGCCCTGCTGCAGCCTCTGTGGTACAGCCTGAGAACCCTGTCCCGG CTGCCCAGGACACCACTGACAAGAAGCCCACCAGGCCAGCAGCAGCATCGCAGGACTCACAAGCGGCTGA CGGAGCAGGCAGAGCCTCTTTGGCCACGAAGAGGAAAGAGGACCCCGCAGACCCTGAAGTCAACCAGAGG AACCTCCAGTTTGTGGGAGTCAATGGCGGGTATGAGTCCCCCTCGGAGGACTCCAGCACAGCAGAAAACT CCGAACACGAGAGCACAGAGAACGAGGGCCCGGAGCCACCAGCAAGGGTTCTGAGCCCAGCTGAATGTCC TCAGCTCAGGCCTCCAGGGGCTGCAGTGGCCACGACCAGTCTGGAGGGGCCCCAGCTCTCCCAGGAATCC CAGCGTGTGCCTGCACCTGAGGTGGCGTCAGGACCCGATCCTGAGGAGGAAATTCGAAAGTGGCCTATAC CACAGTGCTTCAGGAGTGGCTGCGCCTGGCCTGCCGCAGTGACGCACACCCTGAGCTTGTGCGGCGACAT C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209360 representing BC010245
Red=Cloning site Green=Tags(s) MAQVLHVPAPFPGTPGQASPAAFPSKEPDPPYSVETPYGYRLDLDFLKYVDDIEKGHTLRRVAVQRRPRL GSLPRGPGSWWTSTESLCSDASGDSRHSAYSYCGRGFYPQYGALETRIGSNPRVERTLLDARRRLEDQVA APSSGGLGSLTPSAAGSTSSLAGVGLLPPTPRSSGLSTPVAPSAGHLAHVREQMAGALRKLRQLEEQVKL IPVLQVKLSVLQEEKRQLTVQLKSQKFLGHPSGTRSRSELCLDLPEAPDDPAALETRSVGTWVRERDLGI PDGEAALVAKVAVLETQLKKALQELRAAQTQQVDLQPQAWPPPDTQVRVDTVRVVEGPREVEVAASTAAG ALAQRAQSLEPYGTGLKALTTSGGPENTLVFRSHEVVETMCPLPTATTGNVHTAKKISITERSCTGAPRM TEPSSVNPRPAAASVVQPENPVPAAQDTTDKKPTRPAAASQDSQAADGAGRASLATKRKEDPADPEVNQR NLQFVGVNGGYESPSEDSSTAENSEHESTENEGPEPPARVLSPAECPQLRPPGAAVATTSLEGPQLSQES QRVPAPEVASGPDPEEEIRKWPIPQCFRSGCAWPAAVTHTLSLCGDI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC010245 |
| ORF Size | 1821 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC010245.1 |
| RefSeq Size | 3427 bp |
| RefSeq ORF | 1823 bp |
| Locus ID | 235041 |
| MW | 125.6 kDa |
| Gene Summary | Involved in transcription regulation by sequestering in the cytoplasm nuclear receptor coactivators such as NCOA1, NCOA2 and NCOA3 (By similarity). Involved in regulation of caspase-independent apoptosis by sequestering the proapoptotic factor AIFM1 in mitochondria (By similarity). Pro-apoptotic stimuli can induce its proteasomal degradation allowing the translocation of AIFM1 to the nucleus to induce apoptosis (By similarity). Involved in the negative control of vitamin D receptor signaling pathway (By similarity). Involved in actin stress fibers formation through its interaction with ARHGDIA and the regulation of the Rho signaling pathway (PubMed:25961457). May thereby play a role in cell adhesion and migration, regulating for instance podocytes migration during development of the kidney (PubMed:25961457). Through the Rho signaling pathway may also regulate cell proliferation (PubMed:16024821).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218622 | Ankrd25 (untagged) - Mouse ankyrin repeat domain 25 (cDNA clone MGC:12143 IMAGE:3710765), (10ug) |
CNY 6,940.00 |
|
| MG209360 | Ankrd25 (tGFP-tagged) - Mouse ankyrin repeat domain 25 (cDNA clone MGC:12143 IMAGE:3710765) |
CNY 6,650.00 |
|
| MR209360L3 | Lenti ORF clone of Ankrd25 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 25 (cDNA clone MGC:12143 IMAGE:3710765) |
CNY 7,980.00 |
|
| MR209360L4 | Lenti ORF clone of Ankrd25 (mGFP-tagged) - Mouse ankyrin repeat domain 25 (cDNA clone MGC:12143 IMAGE:3710765) |
CNY 7,980.00 |
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