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Prc1 (NM_145150) Mouse Tagged ORF Clone
CAT＃: MR209337
- TrueORF®
Prc1 (Myc-DDK-tagged) - Mouse protein regulator of cytokinesis 1 (Prc1)
ORF Plasmid: tGFP
"NM_145150" in other vectors (3)
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CNY 4,496.00
CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D7Ertd348e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209337 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGAAGTGAGGTGCTAGCAGATGAGTCTATCACATGCCTGCAGAAAGCTCTAACTCACCTCCGGG AAATATGGGAACTAATTGGGATTCCAGAGGAGCAGAGGCTACAAAGAACCGAGGTTGTAAAGAAGCATAT AAAGGATCTTCTGGATAGAATGATTGCTGAAGAGGAGAGCCTGAGGGAAAGGCTGCTTAAAAGCATATCC ATCTGTCAGAAGGAGCTGAGTACCCTGTGCAGTGAGCTACAAGTGAAGCCATTTCAGGAAGAAAAAGATA CAACCATCTTGCAACTAGAAAAAGATCTGCGCACTCAAGTAGAATTGATGAGAAAACAGAAAAAGGAGAG AAAGCAGGAATTGAAGCTACTTCAGGAACAGGAGCAAGAACTCCGTGACATCCTCTGTATGCCGCCCTGT GATGTGGACAGCACCTCTGTCCCCACCTTAGAAGAGCTGAAGCTATTCCGACAGCGTGTGGCAACGCTGA GGGAGACAAAGGAATCGAGGCGTGAAGAATTTGTCAACATTAAGAAACAAATCATATTGTGTATGGAAGA ATTAGAACACTCTCCAGATACAAGCTTTGAAAGAGATGTGGTGTGTGAAGATGAAAGTGCTTTTTGTTTA TCACTGGAGAACATTGCAACATTACAGAAGTTGCTGAAGCAGCTGGAAATGAAAAAATCACAAAATGAAG CAGAATGTGAGGGGCTTCGCACTCAAATCCGAGAGCTCTGGGATAGGTTACAAATACCTGAAGAAGAGAG AGAGCCTGTGGAGGCAATTATGACTGGATCAAAAACCAAAATCAGGAATGCGCTGAAATTAGAAGTGGAT CGGTTAGAAGAACTGAAAATGCAAAACATAAAGCAAGTGATTGAGAAAATCCGAGTGGAGCTGGCTCAGT TCTGGGACCAGTGTTTTTATAGCCAGGAACAGAGGCAGGCTTTTGCCCCTTACTATTCTGAGGACTACAC AGAAAACCTGCTCCATCTTCATGATGCCGAGATTGTACGGTTAAGAAACTACTATGACGCTCACAAGGAG CTGTTCCAAGGCGTCCAGAAGTGGGAAGAGAGCTGGAAACTTTTCCTAGAGTTTGAGAGAAAAGCTTCAG ATCCAGGTCGGTTTACAAACCGAGGGGGAAATCTTTTAAAAGAAGAAAAGGAACGAGCAAAGCTCCAGAA AACACTCCCTAAGCTGGAAGAGGAGTTGAAAGCACGGATTGAACAGTGGGAACAGGAGCACTCAACAGCA TTTGTGGTGAATGGGCAGAAATTCATGGAGTATGTTACAGAACAGTGGGAATTACATCGGCTGGAGAAAG AGCGAGCCAAACAGGAGAGGCAACTGAAGAACAAGAAACAGACAGAGGCAGAGATGCTGTATGGCAGTAC TCCCCGGACACCTAGCAAAAGACCAGGACAGACGCCCAAGAAGTCTGGCAAAATGAACACTACCACCATG TCTAGTGCTACACCCAACAGTAGCATTCGGCCTGTCTTTGGTGGATCAGTGTACCGGTCACCTATGTCTC GACTACCCCCTTCTGGCAGCAAGTCAGTTGTCACTTCTCTGTGTTCTGGAAAGAAAACACCTCGAGCTGC CCAGCTTAGGGCCAACAAGGAGAACTTGGACCTCAATGGCAGCATCCTGAGCGGTGGGTACCCTGGCTCG ACGCCCCTCCAGCACAACTGCAGCATTAAGTCTGTTGCCAGCACCTATTCTGAGTTTTCGCGAGAACTTT CAAAGGCTTCCAGATCTGATGCTACGTCTCGAATCCTCAATTCAACCAACATCCAGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209337 protein sequence
Red=Cloning site Green=Tags(s) MRRSEVLADESITCLQKALTHLREIWELIGIPEEQRLQRTEVVKKHIKDLLDRMIAEEESLRERLLKSIS ICQKELSTLCSELQVKPFQEEKDTTILQLEKDLRTQVELMRKQKKERKQELKLLQEQEQELRDILCMPPC DVDSTSVPTLEELKLFRQRVATLRETKESRREEFVNIKKQIILCMEELEHSPDTSFERDVVCEDESAFCL SLENIATLQKLLKQLEMKKSQNEAECEGLRTQIRELWDRLQIPEEEREPVEAIMTGSKTKIRNALKLEVD RLEELKMQNIKQVIEKIRVELAQFWDQCFYSQEQRQAFAPYYSEDYTENLLHLHDAEIVRLRNYYDAHKE LFQGVQKWEESWKLFLEFERKASDPGRFTNRGGNLLKEEKERAKLQKTLPKLEEELKARIEQWEQEHSTA FVVNGQKFMEYVTEQWELHRLEKERAKQERQLKNKKQTEAEMLYGSTPRTPSKRPGQTPKKSGKMNTTTM SSATPNSSIRPVFGGSVYRSPMSRLPPSGSKSVVTSLCSGKKTPRAAQLRANKENLDLNGSILSGGYPGS TPLQHNCSIKSVASTYSEFSRELSKASRSDATSRILNSTNIQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145150 |
| ORF Size | 1812 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145150.1, NP_660132.1 |
| RefSeq Size | 3049 bp |
| RefSeq ORF | 1821 bp |
| Locus ID | 233406 |
| MW | 70.3 kDa |
| Gene Summary | Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209337 | Prc1 (tGFP-tagged) - Mouse protein regulator of cytokinesis 1 (Prc1) |
CNY 5,890.00 |
|
| MR209337L3 | Lenti ORF clone of Prc1 (Myc-DDK-tagged) - Mouse protein regulator of cytokinesis 1 (Prc1) |
CNY 7,220.00 |
|
| MR209337L4 | Lenti ORF clone of Prc1 (mGFP-tagged) - Mouse protein regulator of cytokinesis 1 (Prc1) |
CNY 6,896.00 |
