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Ell (NM_007924) Mouse Tagged ORF Clone
CAT＃: MR209325
- TrueORF®
Ell (Myc-DDK-tagged) - Mouse elongation factor RNA polymerase II (Ell)
ORF Plasmid: tGFP
"NM_007924" in other vectors (4)
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CNY 6,736.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ell1; Men |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209325 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCTGAAGGAGGCTAGGAGTTACGGGTTGTCGTGTGGGCGCGTGAGCGACGGCAGCAGGGTGT CGGTGTTCCACGTGAAGCTCACCGACAGTGCTTTGAAGGCTTTTGAGAGCTACCGGGCCCACCAGGATTC TGTGTCACTGAGACCTTCCATCCGATTTGAAGGAAGCCAAGGACACATCTCTATACCCCAGCCTGACTGC CCAGAGGAGGTGCGGGCCTTCTCCTTCTACCTCTCCAATATTGGCCGCGACAGCCCTCAGGGCAGCTTTG ATTGCATCCAACAATATGTATCCAGCCATGGGGATGTACACCTGGACTGCCTGGGCAGCATCCAGGACAA GGTCACAGTGTGTGCTACTGATGACTCCTACCAGAAAGCACGACAGAGCATGGCACAGGCAGAGGAGGAG ACTCGGAGCCGAAGTGCCATCGTCATTAAGGCTGGAGGCCGATACATGGGGAAAAAGGTTCAGTTTCGGA AGCCAGCGCCAGGGGCAGCTGATGCAGTACCCTCCCGGAAGCGTGCTACCCCCATTAACCTGGCAAGTGC CATCAGAAAGAGCAGTGGGAGTGGAGCCAGCAGTGTGGTACAGAGGCCCTTCCGAGATCGGGTGCTACAC CTCCTGGCCCTGAGGCCCTACAGGAAGGCTGAGCTGCTGCTGCGGTTGCAGAAGGATGGGTTGACACAGG CAGACAAGGACACCCTGGACAGCCTGCTGCAGCAGGTGGCCAGTGTGAACCCCAAGGATGGCACGTGCAC GCTGCAGGACTGCATGTACAAAAGCCTGCAGAAGGACTGGCCCGGCTACTCTGAGGGGGACCGGCAGCTG CTGAAGCGCATGCTCATGCGGAAGCTGTGTCAGCCACAGAATGCCACTACAGACTCCAGCCCGCCCCGAG AGCATGGACGCTCTGCCTCACCCTCTCAGAAACGGCCTACAGACTTCATTGACCCCCTGGCCAGCAAGAA GCCCCGGATCTCACATTTCACACAGCGAGCACAACCCACCCTCAATGGCAAACTGGGTGCCCCCAATGGC CATGAGACACTGCTGCCTGCTCCAGGACCCACCCCATCAGACACCCTCAGCTCTAGCCATCTGCCCCCAC GGCTGGAGCCCCCAAGGACCCACGACCCCCTAGCTGATGTCAGTAATGACCTAGGTCACAGTACCCAGGA CTACAAGCACCAGGAAGCCACCCCAGCTCCAGCACCCCATCTTGGTCTTCCCCTGCTGACGGACTTTCCT CAGGCTGAGCAACCTACTAGCTCCTCACACACCCACAGCCGACCCAAGAAGAAGTCCAAGAAGCACAAAG ACAAGGAGCGGCCCCCTGAAGAAAGGCCCCCCGCCCCACAGCCTGATGCACCTACTGCCCCTGCACTACC GCCAGATGCCCCAGGTCTGAATGGAGCCTGTGACAATGAACCCACATCCTCGTCAGAGACCCCGGACTAT TTGCTGAAATACCCAGCCATCTCGTCGTCAGAGCAGCGTCAGAGCTACAAGAATGACTTCAATGCTGAGT ACAGCGAGTACCGCAGTCTGCACGCGAGGATCGAGCAGATCACCCGCAGGTTCACCCAGCTTGATGCACA GCTCAGACAGCTGAGCCAGGGCTCTGATGAGTACGAGACAACCCGTGGGCAAATTCTTCAGGAATACAGG AAAATCAAAAAGACCAACACCAACTACAGCTGTGAGAAGCGACGCTGCGAATACCTGCACCGCAAGCTGG CGCACATTAAGAGGCTCATCGCAGAGTATGACCAGCGGCAGCTTCAGGCCTGGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209325 protein sequence
Red=Cloning site Green=Tags(s) MAALKEARSYGLSCGRVSDGSRVSVFHVKLTDSALKAFESYRAHQDSVSLRPSIRFEGSQGHISIPQPDC PEEVRAFSFYLSNIGRDSPQGSFDCIQQYVSSHGDVHLDCLGSIQDKVTVCATDDSYQKARQSMAQAEEE TRSRSAIVIKAGGRYMGKKVQFRKPAPGAADAVPSRKRATPINLASAIRKSSGSGASSVVQRPFRDRVLH LLALRPYRKAELLLRLQKDGLTQADKDTLDSLLQQVASVNPKDGTCTLQDCMYKSLQKDWPGYSEGDRQL LKRMLMRKLCQPQNATTDSSPPREHGRSASPSQKRPTDFIDPLASKKPRISHFTQRAQPTLNGKLGAPNG HETLLPAPGPTPSDTLSSSHLPPRLEPPRTHDPLADVSNDLGHSTQDYKHQEATPAPAPHLGLPLLTDFP QAEQPTSSSHTHSRPKKKSKKHKDKERPPEERPPAPQPDAPTAPALPPDAPGLNGACDNEPTSSSETPDY LLKYPAISSSEQRQSYKNDFNAEYSEYRSLHARIEQITRRFTQLDAQLRQLSQGSDEYETTRGQILQEYR KIKKTNTNYSCEKRRCEYLHRKLAHIKRLIAEYDQRQLQAWP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007924 |
| ORF Size | 1809 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007924.1 |
| RefSeq Size | 3085 bp |
| RefSeq ORF | 1809 bp |
| Locus ID | 13716 |
| UniProt ID | O08856 |
| MW | 67.1 kDa |
| Gene Summary | Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription. ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation. Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription (By similarity). Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202922 | Ell (untagged) - Mouse elongation factor RNA polymerase II (Ell), (10ug) |
CNY 6,736.00 |
|
| MG209325 | Ell (tGFP-tagged) - Mouse elongation factor RNA polymerase II (Ell) |
CNY 8,336.00 |
|
| MR209325L3 | Lenti ORF clone of Ell (Myc-DDK-tagged) - Mouse elongation factor RNA polymerase II (Ell) |
CNY 7,890.00 |
|
| MR209325L4 | Lenti ORF clone of Ell (mGFP-tagged) - Mouse elongation factor RNA polymerase II (Ell) |
CNY 7,890.00 |
