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Kcna5 (NM_145983) Mouse Tagged ORF Clone
CAT＃: MR209320
- TrueORF®
Kcna5 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, member 5 (Kcna5)
ORF Plasmid: tGFP
"NM_145983" in other vectors (3)
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CNY 4,488.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Kv1.5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209320 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATCTCCCTGGTGCCCATGGAGAACGGCAGTGCCATGACCCTCAGAGGAGGAGGGGAGGCAGGGG CAAGCTGTGTGCAGAGCCCCAGGGGAGAGTGTGGGTGCCCTCCGACGGCTGGACTCAATAATCAGTCCAA AGAAACATCACCGAGGAGGCGCGCCACACACGAGGATGCGGGCCAGGGTGGGAGGCCTTTGCCCCCTATG CCTCAGGAGCTGCCACAGCCTAGAAGGCCATCTGCTGAGGATGAGGAGGGAGAAGGCGACCCTGGCCTGG GCACAGTGGAGGAGGACCAGGCTCCTCAGGACTCAGGATCACTCCATCACCAGCGGGTCCTCATAAACAT CTCGGGTTTGCGCTTTGAGACGCAGCTGGGCACCCTGGCGCAGTTTCCCAACACCCTCCTGGGGGACCCA GTCAAACGCCTGCGCTACTTCGATCCCTTGAGAAATGAGTACTTCTTCGACCGCAACCGGCCTAGCTTCG ATGGCATTTTGTACTACTACCAGTCTGGGGGCCGCCTGCGCAGGCCGGTCAATGTCTCCCTGGATGTGTT TGCAGATGAGATCCGCTTTTACCAGCTGGGGGACGAGGCCATGGAGCGCTTCCGGGAGGATGAAGGCTTC ATCAAGGAAGAGGAGAAGCCCCTGCCCCGCAATGAGTTCCAGCGCCAGGTGTGGCTTATCTTCGAATACC CAGAAAGCTCTGGGTCAGCAAGAGCCATTGCCATCGTGTCGGTCTTGGTCATTCTCATCTCTATCATCAC CTTCTGCTTGGAGACTCTGCCTGAGTTCAGGGATGAACGGGAGCTGTTACGCCACCCCCCAGTGCCGCCG CAGCCCCCAGCCCCTGCCCCAGGGGCAAATGGCAGTGGCTCTGGTGTCCTTTCCTCTGGCCCCACAGTGG CTCCGCTCCTGCCTAGGACACTGGCTGACCCATTCTTCATCGTGGAGACCACATGTGTGATCTGGTTCAC TTTTGAGCTGCTTGTGCGTTTCTTTGCTTGCCCCAGCAAGGCAGAATTCTCTCGGAATATTATGAACATC ATTGATATTGTGGCCATCTTCCCCTACTTTATCACCCTGGGCACCGAGCTGGCAGAGCAACAACCAGGGG GTGGTGGTCAGAATGGGCAGCAGGCCATGTCCCTAGCCATCCTCAGGGTGATCCGCCTGGTCCGGGTGTT CCGAATCTTCAAGCTCTCCCGCCACTCCAAGGGACTGCAGATCCTGGGTAAGACCTTACAGGCGTCCATG CGGGAGCTCGGGCTGCTCATCTTCTTCCTCTTCATCGGAGTCATCCTCTTCTCCAGCGCTGTCTACTTCG CAGAGGCAGACAATCAGGGGTCGCACTTCTCCAGTATCCCGGATGCATTCTGGTGGGCAGTAGTCACTAT GACCACTGTAGGCTACGGGGACATGAGGCCCATCACTGTAGGGGGCAAGATTGTGGGCTCACTGTGCGCC ATAGCTGGGGTCCTCACCATTGCTCTGCCTGTGCCCGTCATCGTCTCCAATTTTAATTACTTCTATCATC GGGAGACAGACCACGAGGAGCAGGCTGCCCTGAAGGAAGAGCAAGGCATCCAGAGGCGGGAGTCTGGGCT GGACACAGGAGGTCAACGGAAGGTCAGCTGCAGCAAGGCCTCCTTCTGCAAGACTGGGGGACCCCTGGAG AGTACTGACAGTATCAGAAGGGGTAGCTGTCCTCTAGAAAAGTGTCACCTAAAGGCCAAGAGCAACGTGG ACCTGCGAAGGTCTCTGTATGCCCTCTGTCTGGACACTAGCCGGGAAACAGATTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209320 protein sequence
Red=Cloning site Green=Tags(s) MEISLVPMENGSAMTLRGGGEAGASCVQSPRGECGCPPTAGLNNQSKETSPRRRATHEDAGQGGRPLPPM PQELPQPRRPSAEDEEGEGDPGLGTVEEDQAPQDSGSLHHQRVLINISGLRFETQLGTLAQFPNTLLGDP VKRLRYFDPLRNEYFFDRNRPSFDGILYYYQSGGRLRRPVNVSLDVFADEIRFYQLGDEAMERFREDEGF IKEEEKPLPRNEFQRQVWLIFEYPESSGSARAIAIVSVLVILISIITFCLETLPEFRDERELLRHPPVPP QPPAPAPGANGSGSGVLSSGPTVAPLLPRTLADPFFIVETTCVIWFTFELLVRFFACPSKAEFSRNIMNI IDIVAIFPYFITLGTELAEQQPGGGGQNGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGKTLQASM RELGLLIFFLFIGVILFSSAVYFAEADNQGSHFSSIPDAFWWAVVTMTTVGYGDMRPITVGGKIVGSLCA IAGVLTIALPVPVIVSNFNYFYHRETDHEEQAALKEEQGIQRRESGLDTGGQRKVSCSKASFCKTGGPLE STDSIRRGSCPLEKCHLKAKSNVDLRRSLYALCLDTSRETDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145983 |
| ORF Size | 1809 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145983.2 |
| RefSeq Size | 3032 bp |
| RefSeq ORF | 1809 bp |
| Locus ID | 16493 |
| UniProt ID | Q61762 |
| MW | 66.6 kDa |
| Gene Summary | Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:8226976, PubMed:11349004). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (By similarity). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (By similarity). Homotetrameric channels display rapid activation and slow inactivation (PubMed:8226976, PubMed:11349004). May play a role in regulating the secretion of insulin in normal pancreatic islets (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209320 | Kcna5 (tGFP-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, member 5 (Kcna5) |
CNY 5,800.00 |
|
| MR209320L3 | Lenti ORF clone of Kcna5 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, member 5 (Kcna5) |
CNY 7,130.00 |
|
| MR209320L4 | Lenti ORF clone of Kcna5 (mGFP-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, member 5 (Kcna5) |
CNY 7,130.00 |
