United States
Germany
Japan
United Kingdom
China
Ppm1d (BC023492) Mouse Tagged ORF Clone
CAT＃: MR209279
- TrueORF®
Ppm1d (Myc-DDK-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376)
ORF Plasmid: tGFP
"BC023492" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 4,448.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AV338790; Wip1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209279 representing BC023492
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCGGACCAGGGCGGGAGGAAGTACATGGAGGACG TAACTCAGATCGTGGTGGAGCCCGAGCCGGCTGCGGAGGACAAGCCGGCGCCGGTACCGCGGCGGGCGCT CGGGTTGCCGGCGACCCCCACTCTCGCCGGCGTCGGGCCATCGGAAAAAGGCCCGGCGGCGGCCCGCGAC CCTGCCCCGGACGCCGCGGCCTCGCTACCCGCTGGCCGCTGCTGTCGCCGCCGCTCTTCGGTGGCCTTCT TTGCAGTGTGCGACGGGCACGGCGGTCGGGAGGCGGCACAGTTTGCCCGGGAGCACTTGTGGGGTTTCAT CAAGAAGCAGAAAGGCTTCACCTCGTCCGAGCCCGCCAAGGTGTGCGCTGCCATCCGCAAAGGTTTCCTC GCCTGTCACCTCGCCATGTGGAAGAAACTGGAATGGCCAAAGACTATGACAGGTCTTCCCAGCACGTCCG GGACAACTGCCAGTGTGGTTATAATACGGGGCATGAAGATGTATGTAGCGCATGTAGGTGACTCTGGGGT GGTCCTTGGAATTCAGGATGACCCAAAGGATGATTTTGTGAGAGCTGTGGAGGTGACACAAGATCACAAG CCAGAACTTCCCAAGGAAAGAGAAAGAATCGAAGGACTTGGCGGCAGTGTGATGAACAAGTCTGGAGTGA ACCGAGTAGTTTGGAAAAGGCCCCGGCTCACTCACAGTGGACCTGTCAGAAGGAGCACAGTCATTGACCA GATTCCTTTTCTGGCTGTAGCAAGAGCGCTTGGTGACTTGTGGAGCTATGATTTCTTCAGTGGTAAGTTT GTGGTGTCACCTGAACCAGACACCAGTGTCCACACTCTTGACCCCCGGAAGCACAAGTATATTATCCTGG GAAGTGATGGACTTTGGAATATGGTTCCACCTCAAGATGCCATCTCCATGTGCCAAGACCAAGAGGAGAA AAAATACTTGATGGGTGAGCAAGGACAGTCCTGTGCCAAAATGCTTGTGAATCGAGCACTTGGCCGCTGG AGGCAGCGTATGCTTCGGGCAGATAACACAAGTGCCATCGTAATCTGCATCTCTCCAGAAGTAGACAACC AAGGGAACTTCACCAATGAAGATGAGCTCTTTCTGAACCTGACTGATAGCCCTACTTACAACAGCCAGGA GACCTGTGTGATGACTTCTTCTCCAAGTTCTACACCACCAATCAAGTCACCGGAAGAAGATGCATGGCCA AGGCTGAGCTCTAAGGACCATATACCTGCCCTTGTTCGCAGTAATGCCTTCTCAGAGAAGTTTTTAGAGG TCCCAGCTGAGATAGCTAGAGGGAATATCCAGACTGTAGTGATGACCTCAAAAGACTCAGAGACACTTGA AGAAAATTGCCCCAAAGCCCTGACTTTAAGGATTCATGATTCTTTGAATAATACTCTGTCAGTTGGCCTC ATTCCAACCAATTCAACAAATACTATCATGGACCAAAAAAACTTAAAGATGTCAACTCCAGGTCAAATGA AAGCTCAAGAAGTTGAAAGAACCCCTCCAGCCAATTTTAAAAGGACATTAGAAGAATCCAACTCTGGCCC CCTTATGAAGAAGCACCGACGAAATGGCTTAAGTCGAAGTAGCGGGGCCCAGGCTTCCAGTCTCCCTACA GCATCCCAGCGCAGGCACTCTGTCAAACTGACCCTGAGGCGCAGACTCAGGGGCCAGAGGAAGATGGGAA ATCCTCTTCTCCACCAGCACCGGAAAACAGTGTGTGTGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209279 representing BC023492
Red=Cloning site Green=Tags(s) MAGLYSLGVSVFSDQGGRKYMEDVTQIVVEPEPAAEDKPAPVPRRALGLPATPTLAGVGPSEKGPAAARD PAPDAAASLPAGRCCRRRSSVAFFAVCDGHGGREAAQFAREHLWGFIKKQKGFTSSEPAKVCAAIRKGFL ACHLAMWKKLEWPKTMTGLPSTSGTTASVVIIRGMKMYVAHVGDSGVVLGIQDDPKDDFVRAVEVTQDHK PELPKERERIEGLGGSVMNKSGVNRVVWKRPRLTHSGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGKF VVSPEPDTSVHTLDPRKHKYIILGSDGLWNMVPPQDAISMCQDQEEKKYLMGEQGQSCAKMLVNRALGRW RQRMLRADNTSAIVICISPEVDNQGNFTNEDELFLNLTDSPTYNSQETCVMTSSPSSTPPIKSPEEDAWP RLSSKDHIPALVRSNAFSEKFLEVPAEIARGNIQTVVMTSKDSETLEENCPKALTLRIHDSLNNTLSVGL IPTNSTNTIMDQKNLKMSTPGQMKAQEVERTPPANFKRTLEESNSGPLMKKHRRNGLSRSSGAQASSLPT ASQRRHSVKLTLRRRLRGQRKMGNPLLHQHRKTVCVC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC023492 |
| ORF Size | 1791 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC023492.1 |
| RefSeq Size | 2023 bp |
| RefSeq ORF | 1793 bp |
| Locus ID | 53892 |
| MW | 74.1 kDa |
| Gene Summary | Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation (By similarity). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (PubMed:24135283).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Wip1 phosphatase modulates both long-term potentiation and long-term depression through the dephosphorylation of CaMKII
,He, ZY;Hu, WY;Zhang, M;Yang, ZZ;Zhu, HM;Xing, D;Ma, QH;Xiao, ZC;,
Cell Adh Migr
,PubMed ID 27158969
[Ppm1d]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209279 | Ppm1d (tGFP-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376) |
CNY 3,800.00 |
|
| MR209279L3 | Lenti ORF clone of Ppm1d (Myc-DDK-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376) |
CNY 5,320.00 |
|
| MR209279L4 | Lenti ORF clone of Ppm1d (mGFP-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376) |
CNY 5,320.00 |
