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Cry2 (BC054794) Mouse Tagged ORF Clone
CAT＃: MR209229
- TrueORF®
Cry2 (Myc-DDK-tagged) - Mouse cryptochrome 2 (photolyase-like) (cDNA clone MGC:65642 IMAGE:6834104)
ORF Plasmid: tGFP
"BC054794" in other vectors (4)
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CNY 6,608.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AV006279; D130054K12Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209229 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCTGCTGTGGTGGCAGCGACGGTCCCCGCGCAATCGATGGGCGCGGACGGCGCGTCCTCGG TGCACTGGTTCCGCAAAGGACTACGGCTCCACGACAACCCCGCGCTGCTAGCTGCCGTGCGCGGGGCGCG CTGTGTGCGCTGCGTCTACATCCTCGACCCGTGGTTCGCGGCCTCCTCGTCTGTGGGCATCAACCGATGG AGGTTCCTACTGCAATCTCTGGAAGATCTGGACACAAGCTTAAGAAAGCTGAATTCGCGTCTGTTTGTAG TCCGGGGACAGCCAGCTGATGTGTTCCCAAGGCTGTTCAAGGAATGGGGGGTGACCCGTTTGACCTTTGA ATATGACTCTGAACCCTTTGGGAAAGAACGGGATGCAGCCATTATGAAGATGGCCAAGGAGGCTGGCGTG GAGGTGGTGACTGAGAACTCTCACACCCTCTATGACCTAGACAGAATCATCGAACTGAATGGGCAGAAAC CACCCCTTACCTACAAGCGCTTTCAGGCCCTCATCAGCCGCATGGAGCTGCCCAAGAAGCCCGCGGTGGC TGTGAGCAGCCAGCAGATGGAGAGCTGCAGAGCTGAGATCCAGGAGAACCATGACGACACCTATGGCGTG CCTTCCCTGGAGGAGCTGGGATTCCCCACGGAAGGACTTGGCCCAGCTGTTTGGCAAGGAGGAGAGACAG AAGCTCTGGCCCGCCTGGACAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGACCTCGGAT GAATGCCAATTCCTTACTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGATGCCTCTCCTGC CGCCTCTTCTACTACCGCCTGTGGGACTTGTACAAGAAGGTGAAGAGGAACAGCACACCCCCCCTCTCCT TATTTGGACAACTCCTGTGGCGAGAATTCTTCTACACAGCGGCCACCAACAACCCCAGGTTTGACCGAAT GGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGCAACCCCGAAGCCCTGGCCAAGTGGGCCGAG GGCAAGACAGGCTTCCCTTGGATTGACGCCATCATGACCCAACTGAGGCAGGAGGGCTGGATCCACCACC TGGCCCGGCACGCTGTGGCCTGCTTCCTCACCCGCGGGGACCTCTGGGTCAGCTGGGAGAGCGGGGTCCG GGTATTTGACGAGCTGCTCCTGGATGCCGATTTCAGTGTGAATGCAGGCAGCTGGATGTGGCTGTCCTGC AGTGCTTTCTTCCAACAATTCTTCCACTGCTACTGCCCTGTGGGCTTCGGCCGACGTACAGACCCCAGTG GGGACTACATCCGGCGGTACCTGCCCAAACTGAAAGGCTTCCCCTCTCGATACATCTATGAGCCCTGGAA TGCCCCCGAGTCAGTTCAGAAGGCTGCCAAGTGCATCATTGGCGTGGACTACCCACGGCCCATCGTCAAT CATGCAGAGACTAGTCGGCTCAACATTGAACGAATGAAGCAGATCTACCAACAGCTGTCGAGATACCGGG GACTCTGTCTATTGGCATCTGTCCCTTCCTGTGTGGAAGACCTCAGTCACCCTGTGGCAGAGCCTGGTTC AAGCCAAGCTGGGAGCATCAGCAACACAGGCCCCAGAGCACTATCCAGTGGCCCAGCTTCCCCCAAACGC AAGCTGGAAGCAGCCGAGGAACCTCCTGGTGAAGAACTGACCAAGCGGGCTAGAGTGACGGAGATGCCTA CCCAAGAGCCAGCAAGCAAGGACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209229 protein sequence
Red=Cloning site Green=Tags(s) MAAAAVVAATVPAQSMGADGASSVHWFRKGLRLHDNPALLAAVRGARCVRCVYILDPWFAASSSVGINRW RFLLQSLEDLDTSLRKLNSRLFVVRGQPADVFPRLFKEWGVTRLTFEYDSEPFGKERDAAIMKMAKEAGV EVVTENSHTLYDLDRIIELNGQKPPLTYKRFQALISRMELPKKPAVAVSSQQMESCRAEIQENHDDTYGV PSLEELGFPTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMNANSLLASPTGLSPYLRFGCLSC RLFYYRLWDLYKKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRMEGNPICIQIPWDRNPEALAKWAE GKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVSWESGVRVFDELLLDADFSVNAGSWMWLSC SAFFQQFFHCYCPVGFGRRTDPSGDYIRRYLPKLKGFPSRYIYEPWNAPESVQKAAKCIIGVDYPRPIVN HAETSRLNIERMKQIYQQLSRYRGLCLLASVPSCVEDLSHPVAEPGSSQAGSISNTGPRALSSGPASPKR KLEAAEEPPGEELTKRARVTEMPTQEPASKDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC054794 |
| ORF Size | 1776 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC054794, AAH54794 |
| RefSeq Size | 3975 bp |
| RefSeq ORF | 1778 bp |
| Locus ID | 12953 |
| MW | 66.9 kDa |
| Gene Summary | This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217695 | Cry2 (untagged) - Mouse cryptochrome 2 (photolyase-like) (cDNA clone MGC:65642 IMAGE:6834104), (10ug) |
CNY 6,940.00 |
|
| MG209229 | Cry2 (tGFP-tagged) - Mouse cryptochrome 2 (photolyase-like) (cDNA clone MGC:65642 IMAGE:6834104) |
CNY 6,460.00 |
|
| MR209229L3 | Lenti ORF clone of Cry2 (Myc-DDK-tagged) - Mouse cryptochrome 2 (photolyase-like) (cDNA clone MGC:65642 IMAGE:6834104) |
CNY 7,790.00 |
|
| MR209229L4 | Lenti ORF clone of Cry2 (mGFP-tagged) - Mouse cryptochrome 2 (photolyase-like) (cDNA clone MGC:65642 IMAGE:6834104) |
CNY 9,008.00 |
