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Mtpap (NM_026157) Mouse Tagged ORF Clone
CAT＃: MR209138
- TrueORF®
Mtpap (Myc-DDK-tagged) - Mouse mitochondrial poly(A) polymerase (Mtpap), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_026157" in other vectors (4)
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CNY 4,360.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 0610027A18Rik; AW551379; Papd1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209138 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTCGCGGCGTGGGGTTATTGACCCGTTTGCCCGTGTGTTCCCAGAGACGGAACCGAATCCCGC GCTCTATTTCCAGGCTCCTCAGTTGTCCAGGGACTATTGCGGCGAGCATCGGGAGCGAAGAACAGTCTTC GGTGGTTGCGGAAACAGGCATTGAAGACAAGACCCTCCAAAAGAAATTCTCTGAGGTGCAGAAAGAGAGA AGAGAACAGGCCCAGCGGACTGTTTTGATACATTGTCCAAATAATATCAATGAGAAAAAATTTCTCAAGT ATTTATCCCAGCATGGACCAGTCAATAATCATTTCTTCTACGAGAGCTTTGGTCTCTTTGCTGTTGTAGA ATTTTGCCAAAAAGACAGTATAAAGTCATTGCAGAATGGAACACATACTCCAACCCAGAGCACAGAGGCT GCAATTCCATTTAAGTCACGGTTCTTGAACTTAAGGCTGAAGAATCCGTCTAGCCAAGTATCTGGACAGC CATTTGTACAGACAACCAACCAGTCACCTCCTTCAAGCAAAAAGCTCTTTGAATTACTGAGTTATGCAGA AAGCATAGAGGAGCAGCTGAACACTCTCCTGAAGGCCTTCCAGCTCACAGAAGAGAATATCAGGCTCCGC CATCTCACCTGCTCTCTGATTGAGGACATTGCTGCTGCATATTTTCCCAGCTGTGTCATCCGGCCCTTTG GCTCCTCAGTGAACACATTTGGCAAATTAGGATGTGATTTGGACATGTTTTTAGATCTAGATGAAACTGG AAAACTTGATGTTCACAAGAATACAGGGAATTTTTTTATGGAATTTCAAGTGAAAAACGTTCCTTCAGAA AGAATTGCAACCCAGAAGATCCTATCTGTAATAGGAGAGTGCCTTGACAATTTTGGCCCTGGCTGTGTGG GTGTTCAGAAGATACTCAATGCTCGATGTCCACTGGTGAGATTCTCCCATCAGGGCTCTGGATTCCAGTG TGATCTGACTGCTAACAATAGTATTGCTTTGAAAAGTTCCGAACTCCTTTATATATATGGCTCCCTGGAT TCGAGAGTAAGAGCTTTGGTGTTCAGTGTGCGATGCTGGGCTCGGGCACATTCATTAACAAGCAGTATTC CTGGTGCTTGGATTACAAACTTCTCCTTAACAGTGATGGTCATCTTTTTCCTCCAGAGAAGATCACCTCC CATTCTTCCAACTCTAGATTCCCTAAAGTCCATAGCAGATGCTGAAGATAGATGTATTCTAGAGGGGAAC AATTGTACATTCGTCCAGGATGTGAATAAAATTCAACCTTCTGGAAACACAGAAACATTAGAATTACTAA TAAAGGAATTTTTTGAGTATTTTGGCAATTTCGCTTTCAATAAAAATTCCATAAACATTCGACAGGGAAG GGAACAAAACAAGCCTGATTCCTCTCCTCTATACATCCAGAATCCTTTTGAAACATCTCTCAACATAAGC AAAAATGTCAGTCAGAGCCAGCTACAAAAATTTGTGGAACTGGCAAGAGACAGTGCCTGGATTTTGGAAC AGGAAGATAAGAATCAGCCTTTTTCTTCGAGTAGGCAACCCTGGGGACTGGCTGCCCTCCTGCTGCCACC GGGGTCAGGCCACACATCTCTCTCCCGGAAGAAGAAGAAAAAGCCGATGAGTGAAAAAGTCAAAGGCTTG TTAGCATCCATAAAAAGTAACAGCCCTGACAGTTCCACAGATACCAGTGGAAAGAGAACAATTAGTACAC AGGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209138 protein sequence
Red=Cloning site Green=Tags(s) MAARGVGLLTRLPVCSQRRNRIPRSISRLLSCPGTIAASIGSEEQSSVVAETGIEDKTLQKKFSEVQKER REQAQRTVLIHCPNNINEKKFLKYLSQHGPVNNHFFYESFGLFAVVEFCQKDSIKSLQNGTHTPTQSTEA AIPFKSRFLNLRLKNPSSQVSGQPFVQTTNQSPPSSKKLFELLSYAESIEEQLNTLLKAFQLTEENIRLR HLTCSLIEDIAAAYFPSCVIRPFGSSVNTFGKLGCDLDMFLDLDETGKLDVHKNTGNFFMEFQVKNVPSE RIATQKILSVIGECLDNFGPGCVGVQKILNARCPLVRFSHQGSGFQCDLTANNSIALKSSELLYIYGSLD SRVRALVFSVRCWARAHSLTSSIPGAWITNFSLTVMVIFFLQRRSPPILPTLDSLKSIADAEDRCILEGN NCTFVQDVNKIQPSGNTETLELLIKEFFEYFGNFAFNKNSINIRQGREQNKPDSSPLYIQNPFETSLNIS KNVSQSQLQKFVELARDSAWILEQEDKNQPFSSSRQPWGLAALLLPPGSGHTSLSRKKKKKPMSEKVKGL LASIKSNSPDSSTDTSGKRTISTQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026157 |
| ORF Size | 1758 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_026157.3 |
| RefSeq Size | 2651 bp |
| RefSeq ORF | 1758 bp |
| Locus ID | 67440 |
| UniProt ID | Q9D0D3 |
| MW | 65.2 kDa |
| Gene Summary | Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202352 | Mtpap (untagged) - Mouse mitochondrial poly(A) polymerase (Mtpap), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 4,360.00 |
|
| MG209138 | Mtpap (tGFP-tagged) - Mouse PAP associated domain containing 1 (Papd1) |
CNY 5,040.00 |
|
| MR209138L3 | Lenti ORF clone of Mtpap (Myc-DDK-tagged) - Mouse mitochondrial poly(A) polymerase (Mtpap), nuclear gene encoding mitochondrial protein |
CNY 6,460.00 |
|
| MR209138L4 | Lenti ORF clone of Mtpap (mGFP-tagged) - Mouse mitochondrial poly(A) polymerase (Mtpap), nuclear gene encoding mitochondrial protein |
CNY 6,460.00 |
