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Phf19 (NM_028716) Mouse Tagged ORF Clone
CAT＃: MR209063
- TrueORF®
Phf19 (Myc-DDK-tagged) - Mouse PHD finger protein 19 (Phf19)
ORF Plasmid: tGFP
"NM_028716" in other vectors (4)
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CNY 4,304.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 3110009G19Rik; 3321402G02Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209063 representing NM_028716
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACTCAAGCTCTGGAACCAGGGACTCTGGAAGCCTTTGGTGCCACCAGTCCTAACAAGGGGGGCC TGTCTAAGACCAAAAAGAACTTCAAAGACTTGATGTCTAAGGTGACAGAGGGACAGTTCGTGCTATGCAG GTGGACAGACGGGCTATATTACCTTGGCAAGATCAAGCGGGTCAGCAGTCCTAAGCAAAGCTGCCTTGTG ACTTTTGAAGATAATTCCAAATACTGGGTCCTGTGGAAGGACATCCAGCATGCTGGTGTTCCGGGAGAGG AGCCCAAGTGTGACGTCTGCATGGGGAAGACTTCAGGGCCTATGAACGAGATCCTCATCTGTGGGAAGTG TGGCCTGGGTTACCACCAACAGTGCCACATCCCCATCGCAGTTGATGCCAACTGGCCCCTCCTCACTCAT TGGTTCTGCCGACGCTGCATTTTCGCACTGGCTGTGAGGAAAGGTGGCGCTTTGAAGAAAGGCGCCATCG CCAAGACGCTGCAGGCAGTGAAAATGGTGCTGTCCTACCAGCCGGAGGAACTCGATTGGGACTCGCCCCA TCGCACTAACCAGCAGCAATGCTACTGCTACTGCGGCGGGCCTGGAGAGTGGTACCTTCGGATGCTACAG TGCTACCGGTGTAGGCAGTGGTTCCATGAGGCTTGCACACAGTGCCTTAGTGAGCCTATGGTGTTTGGAG ACCGCTTCTACCTGTTCTTCTGCTCCGTGTGTAACCAAGGCCCAGAGTATATTGAGAGGCTGCCCTTGCG ATGGGTGGATATAGTTCACCTGGCTCTCTATAACTTGGGAGTACAGAGCAAGAAGCGGTACTTTGACTTT GAGGAGATCCTGGCCTTTGTCAACCATCACTGGGAGCTCCTGCAGCTTGGCAAGCTCACCAGCACCCCCA TGACAGAACGAGGGCCACATCTCCTCAACGCTCTCAACAGTTACAAGAGCCGGTTCCTGTGTGGCAAGGA AATTAAGAAGAAGAAATGCATCTTCCGACTGCGCATCCGAGTCCCGCCTGCCCCTCCAGGAAAACTGCTT CCCGACAGGGCGTTGATGCCAAGTGACAAAGGGACCTCCGAGCTGCTTCGTAAGAAAGGAAAGAGCAAGC CTGGTTTGTTGCCTCAGGAACCCCAGCAGCAGAAAAGGCGAGTTTATAGAAGAAAAAGATCAAAGTTTTT GCTGGAAGATGCTATTCCCAGTAGTGACTTCACCTCAGCCTGGAGCACAGACCACCACCTAGCCAGTATA TTCGACTTCACACTGGATGAAATTCAGAGTTTAAAAAGTGGCAGCTCAGGCCAGACCTTCTTCTCAGATG TGGATTCTACCGACGCAGCCAGCACCTCGGGGTCTGCCTCCACCAGCCTCTCCTACGACTCCAGATGGAC GGTAGGCAGCCGCAAGAGGAAGCTGACAGCCAAAGTGCACAGGCCCCTGCGAGCAAAGCAAAGGGCGGCG GAGCTGGAGGGGCGCTGCGCCTCAGACAGCAATGCAGAGGGAGCTGTGGGTCCTGAGCAGCCGGATGAAG GCATCGACAGCCACACACTTGAAAGCATCAGTGGAGACGACTCGTCCCTGTCCCACCTCAAGTCCTCTAT CACCAACTACTTTGGTGCAGCTGGGCGGTTGGCCTGCGGGGAAAAATATCGGGTGTTGGCGCGGAGGGTC ACTCCAGAAGGCAAGGTTCAGTACCTGTTGGAATGGGAGGGGACCACCCCTTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209063 representing NM_028716
Red=Cloning site Green=Tags(s) METQALEPGTLEAFGATSPNKGGLSKTKKNFKDLMSKVTEGQFVLCRWTDGLYYLGKIKRVSSPKQSCLV TFEDNSKYWVLWKDIQHAGVPGEEPKCDVCMGKTSGPMNEILICGKCGLGYHQQCHIPIAVDANWPLLTH WFCRRCIFALAVRKGGALKKGAIAKTLQAVKMVLSYQPEELDWDSPHRTNQQQCYCYCGGPGEWYLRMLQ CYRCRQWFHEACTQCLSEPMVFGDRFYLFFCSVCNQGPEYIERLPLRWVDIVHLALYNLGVQSKKRYFDF EEILAFVNHHWELLQLGKLTSTPMTERGPHLLNALNSYKSRFLCGKEIKKKKCIFRLRIRVPPAPPGKLL PDRALMPSDKGTSELLRKKGKSKPGLLPQEPQQQKRRVYRRKRSKFLLEDAIPSSDFTSAWSTDHHLASI FDFTLDEIQSLKSGSSGQTFFSDVDSTDAASTSGSASTSLSYDSRWTVGSRKRKLTAKVHRPLRAKQRAA ELEGRCASDSNAEGAVGPEQPDEGIDSHTLESISGDDSSLSHLKSSITNYFGAAGRLACGEKYRVLARRV TPEGKVQYLLEWEGTTPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028716 |
| ORF Size | 1734 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028716.4 |
| RefSeq Size | 3746 bp |
| RefSeq ORF | 1737 bp |
| Locus ID | 74016 |
| UniProt ID | Q9CXG9 |
| MW | 65.7 kDa |
| Gene Summary | Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds dimethylated at 'Lys-36' (H3K36me2).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204253 | Phf19 (untagged) - Mouse PHD finger protein 19 (Phf19), (10ug) |
CNY 4,312.00 |
|
| MG209063 | Phf19 (tGFP-tagged) - Mouse PHD finger protein 19 (Phf19) |
CNY 5,040.00 |
|
| MR209063L3 | Lenti ORF clone of Phf19 (Myc-DDK-tagged) - Mouse PHD finger protein 19 (Phf19) |
CNY 6,460.00 |
|
| MR209063L4 | Lenti ORF clone of Phf19 (mGFP-tagged) - Mouse PHD finger protein 19 (Phf19) |
CNY 6,704.00 |
