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Chaf1b (NM_028083) Mouse Tagged ORF Clone
CAT＃: MR209007
- TrueORF®
Chaf1b (Myc-DDK-tagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b)
ORF Plasmid: tGFP
"NM_028083" in other vectors (4)
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CNY 2,400.00
CNY 3,696.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2600017H24Rik; C76145; CAF-I p60; CAF-Ip60; CAF1; CAF1A; CAF1P60; MPHOSPH7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209007 representing NM_028083
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCAGGGAACCTCACATGGGTGTCAGAGTTTGTCTTCCTGGGGTTCTCAGAGATCTGGGAGCTTC AGGTCTTCCTGTTTGTGGTGTTCTTGTGTGTCTACAGCACGACCGTGGTGGGAAACCTCCTCATCATAGT TACGGTTTCCTCAGACCCCAGGCTCCACACACCTATGTACTTCCTGCTCCGCAACCTGGCTGTCTTAGAC CTCTGCTTCTCCTCAGTCACTGCCCCCAAGATGCTGGTGGACTTTCTCTCTGAGAAGAAGACTATCTCCT ACCGAGGCTGCATGGTACAGATCTTCTTCTTCCACTTCCTGGGAGGGGCCATGGTCTTCTTCCTCTCAGT AATGGCTTATGACCGCCTGGTGGCTATTTCCCGACCTCTCCACTATGTCACCATCATGAACAGCCAGCTT TGTATGGGGCTGGTGGTGGCTTCCTGGGTAGGAGGCTTTGCCCACTCGATTGTCCAGCTGTCTCTGATGC TCCCGTTGCCGTTCTGTGGACCCAATGTCCTGGATAACTTCTACTGTGATGTCCCCCAAGTACTGAGACT TGCTTGCATGGACACCTCCCTCTTGGAGTTCCTCATGATCTCCAACAGTGGGATGCTGGATGTCATCTGG TTCTTTCTCCTTCTCATCTCCTACTTGGTCATCCTGGTGATGCTGAGGTCTCACTCAGGGGAGGCGAGGA GGAAGGCGGCGTCCACCTGCACCACCCACATCATCGTGGTGTCTATGATCTTCATTCCAAGCATTTACCT GTATGCCCGGCCCTTCACCCCCTTCACCATGGACAAGGCTGTGTCCATCAGCCATACGGTCATGACCCCC ATGCTCAACCCCATGATCTACACGCTCAGGAACCAGGAGATGCAGGCAGCCATGAAGAGACTAGCAAAGC GCTTAGCGCTTTGCAACAGGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209007 representing NM_028083
Red=Cloning site Green=Tags(s) MEPGNLTWVSEFVFLGFSEIWELQVFLFVVFLCVYSTTVVGNLLIIVTVSSDPRLHTPMYFLLRNLAVLD LCFSSVTAPKMLVDFLSEKKTISYRGCMVQIFFFHFLGGAMVFFLSVMAYDRLVAISRPLHYVTIMNSQL CMGLVVASWVGGFAHSIVQLSLMLPLPFCGPNVLDNFYCDVPQVLRLACMDTSLLEFLMISNSGMLDVIW FFLLLISYLVILVMLRSHSGEARRKAASTCTTHIIVVSMIFIPSIYLYARPFTPFTMDKAVSISHTVMTP MLNPMIYTLRNQEMQAAMKRLAKRLALCNRE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028083 |
| ORF Size | 933 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028083.1, NP_082359.1 |
| RefSeq Size | 1913 bp |
| RefSeq ORF | 1719 bp |
| Locus ID | 110749 |
| UniProt ID | Q9D0N7 |
| MW | 63.6 kDa |
| Gene Summary | Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205728 | Chaf1b (untagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b), (10ug) |
CNY 4,264.00 |
|
| MG209007 | Chaf1b (tGFP-tagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b) |
CNY 2,850.00 |
|
| MR209007L3 | Lenti ORF clone of Chaf1b (Myc-DDK-tagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b) |
CNY 4,750.00 |
|
| MR209007L4 | Lenti ORF clone of Chaf1b (mGFP-tagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b) |
CNY 4,750.00 |
