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Dis3 (BC027357) Mouse Tagged ORF Clone
CAT＃: MR208938
- TrueORF®
Dis3 (Myc-DDK-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae) (cDNA clone MGC:37123 IMAGE:4952415)
ORF Plasmid: tGFP
"BC027357" in other vectors (3)
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CNY 4,216.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2810028N01Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208938 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGGTCCAAGACGTTCTTGAAGAAGACCCGCGCGGGCGGCGTGGTGAAGATCGTGCGCGAGCACT ACCTGCGGGATGACATCGGCTGCGGCGCGCCGGCTTGCTCGGCCTGCGGCGGGGCGCACGCGGGCCCGGC CCTGGAGCTGCAGCCCCGGGACCAGGCGAGCAGCCTCTGCCCGTGGCCGCACTACCTTCTGCCGGACACC AATGTGCTGCTGCACCAGATTGATGTCCTCGAACACCCGGCCATCAAAAATGTCATTGTGCTACAAACAG TGATGCAAGAAGTGAGAAACCGGAGCGCCCCCATCTACAAGCGAATCAGGGATGTGACCAATAACCAGGA AAAGCATTTCTATACCTTCACTAATGAGCACCATAAAGAAACCTACATCGAGCAAGAGCAGGGAGAGAAT GCCAATGACAGGAATGACAGAGCCATCCGAGTCGCAGCGAAGTGGTACAACGAGCACCTGAAGAGGGTGG CAGCAGACAGTCAGCTGCAAGTTATCCTGATAACCAATGACAGGAAGAACAAAGAGAAAGCTGTGCAAGA GGGGATACCAGCCTTCACGTGTGAAGAATACGTAAAGAGCCTGACTGCTAACCCTGAACTTATAGACCGT CTTGCTTACTTGTCCGATGAAATGAATGAAATAGAAAGTGGGAAAATAATATTTTCAGAGCATCTTCCCT TAAGCAAGCTCCAACAAGGCATAAAATCTGGTTCCTATCTTCAAGGAACATTCAGAGCTAGCAGGGAAAA TTATTTGGAGGCTACAGTATGGATTCATGGAGACAAAGAAGAGGAAAAAGAGATACTTATACAGGGAATT AAGCATCTAAACAGAGCTGTGCATGAAGACATTGTGGCCGTGGAGCTACTGCCCAGGAGCCAGTGGGTGG CACCGTCTTCCGTGGTTTTAGACGATGAAGGTCAAAATGAAGACGATGTGGAGAAAGATGAGGAGAGAGA ACTCCTGCTTAAGACTGCTGTAAGTGAAAAAATGTTACGGCCTACAGGTCGAGTTGTGGGGATAATAAAA AGGAACTGGAGACCGTATTGTGGCATGCTTTCCAAGTCTGATATTAAGGAGTCAAGAAGACATCTCTTTA CACCCGCTGATAAGAGAATTCCACGAATTCGGATAGAAACCAGACAGGCTTCTGCGTTAGAAGGACGGAG AATTATTGTCGCTATTGATGGTTGGCCTAGAAATTCTAGATATCCAAATGGACACTTTGTAAAGAATTTA GGCGATGTTGGAGAGAAGGAGACAGAAACGGAAGTGTTGCTGCTCGAGCACGATGTTCCTCATCAGCCCT TTTCCCAGGCTGTGCTTAGCTTCCTGCCCAGGATGCCCTGGAGCATTACTGAGGAGGACATGAAAAACCG AGAAGACCTGAGACATCTGTGTGTTTGCAGTGTGGACCCTCCAGGGTGCACTGACATAGATGACGCTCTG CATTGTAGAGAGCTCAGCAATGGAAACTTGGAGGTTGGTGTTCATATTGCGGATGTTAGCCATTTTATCA GGCCAGGAAATGCGTTGGATCAAGAATCTGCAAGAAGAGGAACAACTGTTTATCTTTGTGAAAAGAGGAT TGACATGGTTCCAGAGTTGCTCAGCTCCAACCTCTGTTCCTTAAGATCCAACGTTGACAGGCTTCTCTTA CGTACGCGGAAGCACAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208938 protein sequence
Red=Cloning site Green=Tags(s) MLRSKTFLKKTRAGGVVKIVREHYLRDDIGCGAPACSACGGAHAGPALELQPRDQASSLCPWPHYLLPDT NVLLHQIDVLEHPAIKNVIVLQTVMQEVRNRSAPIYKRIRDVTNNQEKHFYTFTNEHHKETYIEQEQGEN ANDRNDRAIRVAAKWYNEHLKRVAADSQLQVILITNDRKNKEKAVQEGIPAFTCEEYVKSLTANPELIDR LAYLSDEMNEIESGKIIFSEHLPLSKLQQGIKSGSYLQGTFRASRENYLEATVWIHGDKEEEKEILIQGI KHLNRAVHEDIVAVELLPRSQWVAPSSVVLDDEGQNEDDVEKDEERELLLKTAVSEKMLRPTGRVVGIIK RNWRPYCGMLSKSDIKESRRHLFTPADKRIPRIRIETRQASALEGRRIIVAIDGWPRNSRYPNGHFVKNL GDVGEKETETEVLLLEHDVPHQPFSQAVLSFLPRMPWSITEEDMKNREDLRHLCVCSVDPPGCTDIDDAL HCRELSNGNLEVGVHIADVSHFIRPGNALDQESARRGTTVYLCEKRIDMVPELLSSNLCSLRSNVDRLLL RTRKHR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC027357 |
| ORF Size | 1698 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC027357, AAH27357 |
| RefSeq Size | 2996 bp |
| RefSeq ORF | 1700 bp |
| Locus ID | 72662 |
| MW | 64.6 kDa |
| Gene Summary | Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208938 | Dis3 (tGFP-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae) (cDNA clone MGC:37123 IMAGE:4952415) |
CNY 3,520.00 |
|
| MR208938L3 | Lenti ORF clone of Dis3 (Myc-DDK-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae) (cDNA clone MGC:37123 IMAGE:4952415) |
CNY 5,130.00 |
|
| MR208938L4 | Lenti ORF clone of Dis3 (mGFP-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae) (cDNA clone MGC:37123 IMAGE:4952415) |
CNY 5,130.00 |
