Add2 (BC053032) Mouse Tagged ORF Clone

CAT#: MR208912

  • TrueORF®

Add2 (Myc-DDK-tagged) - Mouse adducin 2 (beta) (cDNA clone MGC:62261 IMAGE:6401200)

ORF Plasmid: DDK tGFP



  "BC053032" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,184.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2900072M03Rik; add97
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208912 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGAGGACACGGTTCCCGAGGCGGCCTCCCCGCCACCCTCTCAGGGGCAGCACTACTTTGACCGGT
TCTCTGAAGATGATCCTGAGTACTTGCGACTTCGCAACCGTGCAGCTGACCTGCGACAGGACTTCAACTT
GATGGAGCAGAAGAAGAGGGTCACCATGATCCTGCAGAGCCCTTCTTTCAGGGAGGAGCTGGAAGGCCTC
ATCCAGGAGCAAATGAAGAAGGGCAACAACTCCTCCAACATCTGGGCCCTCCGACAGATCGCGGACTTCA
TGGCCAGCACCTCCCACGCAGTCTTCCCAGCTTCTTCCATGAACTTCTCCATGATGACACCCATCAACGA
CCTCCACACTGCCGACTCCCTGAACCTGGCCAAGGGGGAGAGGCTTATGCGGTGCAAGATCAGCAGCGTC
TACCGTCTCCTGGACCTCTACGGCTGGGCGCAGCTCAGTGACACCTACGTCACGCTGAGAGTGAGCAAGG
AGCAGGACCACTTCCTGATCAGCCCCAAGGGGGTTTCCTGCAGCGAGGTCACAGCCTCCAGCCTGATTAA
AGTGAACATTCTGGGAGAGGTGGTGGAGAAGGGCAGCAGTTGCTTCCCGGTGGACACCACGGGCTTCAGT
CTGCACTCAGCCATCTATGCCGCCAGGCCCGACGTGCGGTGTGCCATCCACCTGCACACGCCTGCCACCG
CAGCGGTGTCAGCTATGAAGTGCGGCCTCCTGCCGGTCTCCCATAATGCCCTGCTGGTGGGGGACATGGC
CTACTATGACTTCAATGGGGAAATGGAGCAGGAAGCTGACCGAATCAACTTGCAGAAGTGCCTTGGACCC
ACCTGCAAGATTCTGGTGCTAAGAAACCATGGCATGGTCGCCCTGGGTGACACCGTGGAGGAAGCTTTCT
ACAAGGTCTTCCATCTGCAGGCTGCGTGTGAGGTACAGGTGTCGGCTCTGTCCAGTGCTGGGGGCACTGA
GAACCTCATCCTCTTGGAGCAAGAGAAACACCGGCCGCACGAGGTGGGCTCTGTGCAGTGGGCCGGCAGC
ACCTTCGGGCCCATGCAGAAGAGCCGGCTGGGAGAGCATGAATTTGAAGCCCTCATGAGGATGCTGGACA
ATTTAGGCTACAGAACAGGCTATACGTACCGCCACCCCTTTGTCCAAGAGAAAACCAAACACAAAAGTGA
AGTGGAGATCCCAGCCACAGTCACTGCCTTTGTGTTTGAAGAGGATGGAGTCCCAGTCCCCGCCCTGCGC
CAGCACGCCCAGAAGCAGCAGAAGGAAAAGACCCGCTGGCTTAACACTCCCAACACCTACCTGCGGGTGA
ACGTGGCTGACGAGGTGCAGAGGAACATGGGCAGTCCCCGACCAAAGACCACGTGGATGAAGGCTGATGA
AGTGGAAAAGTCCAGCAGCGGCATGCCCATACGGATTGAAAACCCAAACCAATTTGTGCCTCTCTACACT
GACCCCCAGGAAGTTCTGGACATGAGGAACAAGATTCGAGAGCAAAACCGACAAGACATAAAGTCAGCCG
GGCCTCAGTCTCAGCTCTTGGCCAGTGTCATCGCAGAGAAGAGCCGGAGTCCGGTACAGCAGAGACTGCC
CCCAACCGAAGGGGAAGTGTATCAGACTCCTGGGGCTGGGCAGGGGACCCCTGAGTCCTCAGGCCCGCTC
ACCCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208912 protein sequence
Red=Cloning site Green=Tags(s)

MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGL
IQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSV
YRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFS
LHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGP
TCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGS
TFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALR
QHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYT
DPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPVQQRLPPTEGEVYQTPGAGQGTPESSGPL
TP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC053032
ORF Size 1686 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC053032, AAH53032
RefSeq Size 3431 bp
RefSeq ORF 1688 bp
Locus ID 11519
MW 62.8 kDa
Gene Summary This gene encodes the beta subunit of the adducin family. Adducins, encoded by alpha, beta and gamma genes, are heteromeric proteins that crosslink actin filaments with spectrin at the cytoskeletal membrane. This protein, primarily found in the brain and hematopoietic cells, is regulated by phosphorylation and calmodulin interactions as it promotes spectrin assembly onto actin filaments, bundles actin and caps barbed ends of actin filaments. In mouse, deficiency of this gene can lead to mild hemolytic anemia and impaired synaptic plasticity. Mutations of this gene in mouse serve as a pathophysiological model for hereditary spherocytosis and hereditary elliptocytosis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Dec 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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