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Add2 (BC053032) Mouse Untagged Clone
CAT＃: MC218134
Add2 (untagged) - Mouse adducin 2 (beta) (cDNA clone MGC:62261 IMAGE:6401200), (10ug)
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2900072M03Rik; add97 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC053032
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAGGACACGGTTCCCGAGGCGGCCTCCCCGCCACCCTCTCAGGGGCAGCACTACTTTGACCGGT TCTCTGAAGATGATCCTGAGTACTTGCGACTTCGCAACCGTGCAGCTGACCTGCGACAGGACTTCAACTT GATGGAGCAGAAGAAGAGGGTCACCATGATCCTGCAGAGCCCTTCTTTCAGGGAGGAGCTGGAAGGCCTC ATCCAGGAGCAAATGAAGAAGGGCAACAACTCCTCCAACATCTGGGCCCTCCGACAGATCGCGGACTTCA TGGCCAGCACCTCCCACGCAGTCTTCCCAGCTTCTTCCATGAACTTCTCCATGATGACACCCATCAACGA CCTCCACACTGCCGACTCCCTGAACCTGGCCAAGGGGGAGAGGCTTATGCGGTGCAAGATCAGCAGCGTC TACCGTCTCCTGGACCTCTACGGCTGGGCGCAGCTCAGTGACACCTACGTCACGCTGAGAGTGAGCAAGG AGCAGGACCACTTCCTGATCAGCCCCAAGGGGGTTTCCTGCAGCGAGGTCACAGCCTCCAGCCTGATTAA AGTGAACATTCTGGGAGAGGTGGTGGAGAAGGGCAGCAGTTGCTTCCCGGTGGACACCACGGGCTTCAGT CTGCACTCAGCCATCTATGCCGCCAGGCCCGACGTGCGGTGTGCCATCCACCTGCACACGCCTGCCACCG CAGCGGTGTCAGCTATGAAGTGCGGCCTCCTGCCGGTCTCCCATAATGCCCTGCTGGTGGGGGACATGGC CTACTATGACTTCAATGGGGAAATGGAGCAGGAAGCTGACCGAATCAACTTGCAGAAGTGCCTTGGACCC ACCTGCAAGATTCTGGTGCTAAGAAACCATGGCATGGTCGCCCTGGGTGACACCGTGGAGGAAGCTTTCT ACAAGGTCTTCCATCTGCAGGCTGCGTGTGAGGTACAGGTGTCGGCTCTGTCCAGTGCTGGGGGCACTGA GAACCTCATCCTCTTGGAGCAAGAGAAACACCGGCCGCACGAGGTGGGCTCTGTGCAGTGGGCCGGCAGC ACCTTCGGGCCCATGCAGAAGAGCCGGCTGGGAGAGCATGAATTTGAAGCCCTCATGAGGATGCTGGACA ATTTAGGCTACAGAACAGGCTATACGTACCGCCACCCCTTTGTCCAAGAGAAAACCAAACACAAAAGTGA AGTGGAGATCCCAGCCACAGTCACTGCCTTTGTGTTTGAAGAGGATGGAGTCCCAGTCCCCGCCCTGCGC CAGCACGCCCAGAAGCAGCAGAAGGAAAAGACCCGCTGGCTTAACACTCCCAACACCTACCTGCGGGTGA ACGTGGCTGACGAGGTGCAGAGGAACATGGGCAGTCCCCGACCAAAGACCACGTGGATGAAGGCTGATGA AGTGGAAAAGTCCAGCAGCGGCATGCCCATACGGATTGAAAACCCAAACCAATTTGTGCCTCTCTACACT GACCCCCAGGAAGTTCTGGACATGAGGAACAAGATTCGAGAGCAAAACCGACAAGACATAAAGTCAGCCG GGCCTCAGTCTCAGCTCTTGGCCAGTGTCATCGCAGAGAAGAGCCGGAGTCCGGTACAGCAGAGACTGCC CCCAACCGAAGGGGAAGTGTATCAGACTCCTGGGGCTGGGCAGGGGACCCCTGAGTCCTCAGGCCCGCTC ACCCCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC053032 |
| Insert Size | 1689 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC053032, AAH53032 |
| RefSeq Size | 3431 bp |
| RefSeq ORF | 1688 bp |
| Locus ID | 11519 |
| Gene Summary | This gene encodes the beta subunit of the adducin family. Adducins, encoded by alpha, beta and gamma genes, are heteromeric proteins that crosslink actin filaments with spectrin at the cytoskeletal membrane. This protein, primarily found in the brain and hematopoietic cells, is regulated by phosphorylation and calmodulin interactions as it promotes spectrin assembly onto actin filaments, bundles actin and caps barbed ends of actin filaments. In mouse, deficiency of this gene can lead to mild hemolytic anemia and impaired synaptic plasticity. Mutations of this gene in mouse serve as a pathophysiological model for hereditary spherocytosis and hereditary elliptocytosis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Dec 2012] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208912 | Add2 (tGFP-tagged) - Mouse adducin 2 (beta) (cDNA clone MGC:62261 IMAGE:6401200) |
CNY 3,520.00 |
|
| MR208912 | Add2 (Myc-DDK-tagged) - Mouse adducin 2 (beta) (cDNA clone MGC:62261 IMAGE:6401200) |
CNY 4,184.00 |
|
| MR208912L3 | Lenti ORF clone of Add2 (Myc-DDK-tagged) - Mouse adducin 2 (beta) (cDNA clone MGC:62261 IMAGE:6401200) |
CNY 5,130.00 |
|
| MR208912L4 | Lenti ORF clone of Add2 (mGFP-tagged) - Mouse adducin 2 (beta) (cDNA clone MGC:62261 IMAGE:6401200) |
CNY 5,130.00 |
