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Tpp1 (NM_009906) Mouse Tagged ORF Clone
CAT＃: MR208901
- TrueORF®
Tpp1 (Myc-DDK-tagged) - Mouse tripeptidyl peptidase I (Tpp1)
ORF Plasmid: tGFP
"NM_009906" in other vectors (4)
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CNY 4,192.00
CNY 5,040.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cl; Cln2; LPIC; TPP-1; TPP-I |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208901 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACTCCAAGCCCGCCTCCTAGGGCTCCTTGCTCTCGTCATCGCCGGCAAATGCACTTACAACCCTG AGCCGGACCAGCGGTGGATGCTGCCTCCAGGCTGGGTGTCCCTGGGCCGCGTGGATCCCGAGGAAGAGCT GAGTCTCACTTTTGCGCTGAAACAGCGGAACCTGGAAAGACTCTCGGAGCTGGTGCAGGCTGTGTCGGAT CCTAGCTCTCCTCAATATGGAAAGTACCTAACCCTGGAGGATGTAGCTGAGCTGGTTCAACCATCACCCC TGACCCTCCTCACTGTCCAAAAGTGGCTCTCAGCAGCTGGAGCCCGGAACTGCGATTCAGTGACCACCCA GGACTTTCTGACTTGCTGGCTGAGTGTCCGACAGGCTGAGCTGCTGCTCCCAGGAGCTGAGTTTCATCGC TATGTAGGGGGACCTACAAAGACCCATGTTATAAGGTCCCCACATCCCTACCAGCTTCCCCAGGCCTTGG CCCCTCATGTGGATTTTGTGGGGGGGCTGCACCGTTTCCCCCCTTCATCTCCAAGACAACGTCCAGAACC ACAACAGGTAGGAACTGTTAGCCTGCACTTGGGAGTGACTCCGTCTGTGCTCCGTCAGCGATACAACCTG ACAGCCAAAGATGTGGGCTCAGGCACCACCAACAATAGCCAGGCCTGTGCCCAGTTCCTGGAACAGTACT TCCATAACTCGGATCTGACTGAGTTCATGCGCCTATTCGGTGGCAGTTTTACACACCAGGCCTCAGTAGC AAAAGTTGTTGGAAAGCAAGGGCGAGGCCGAGCTGGGATCGAGGCCAGTCTAGATGTGGAATACCTGATG AGTGCTGGTGCCAATATCTCCACTTGGGTCTACAGTAGCCCTGGCCGCCATGAGGCACAGGAGCCCTTCT TACAATGGCTCCTGCTTCTTAGCAATGAGTCATCTTTGCCACATGTACATACTGTGAGTTACGGAGACGA TGAAGACTCCCTCAGCAGCATCTACATCCAGAGAGTCAACACTGAGTTCATGAAGGCTGCTGCTCGGGGT CTCACCCTCCTTTTTGCCTCAGGTGACACTGGAGCTGGGTGTTGGTCTGTCTCCGGAAGACACAAGTTCC GCCCTAGCTTCCCTGCTTCCAGCCCCTATGTTACTACAGTTGGAGGAACCTCCTTCAAGAATCCTTTCCT CATCACAGATGAAGTAGTTGACTATATCAGTGGTGGAGGCTTCAGCAATGTTTTCCCACGGCCTCCCTAC CAGGAGGAAGCAGTGGCCCAGTTCTTGAAATCCAGCTCTCATCTACCACCATCCAGTTACTTCAATGCTA GTGGCCGTGCCTACCCAGATGTTGCCGCACTATCTGATGGCTACTGGGTGGTCAGCAACATGGTCCCCAT TCCATGGGTATCTGGAACCTCGGCCTCTACTCCAGTGTTTGGGGGAATTTTATCCTTGATAAATGAGCAC AGAATCCTCAATGGCCGCCCTCCTCTTGGCTTTCTCAACCCCAGGCTCTATCAGCAGCATGGGACAGGAC TCTTTGATGTAACCCACGGCTGCCATGAGTCCTGTCTGAATGAAGAAGTGGAGGGTCAGGGTTTCTGCTC TGGTCCTGGCTGGGATCCTGTGACAGGTTGGGGAACACCCAACTTCCCAGCCCTACTGAAGACCCTGCTC AACCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208901 protein sequence
Red=Cloning site Green=Tags(s) MGLQARLLGLLALVIAGKCTYNPEPDQRWMLPPGWVSLGRVDPEEELSLTFALKQRNLERLSELVQAVSD PSSPQYGKYLTLEDVAELVQPSPLTLLTVQKWLSAAGARNCDSVTTQDFLTCWLSVRQAELLLPGAEFHR YVGGPTKTHVIRSPHPYQLPQALAPHVDFVGGLHRFPPSSPRQRPEPQQVGTVSLHLGVTPSVLRQRYNL TAKDVGSGTTNNSQACAQFLEQYFHNSDLTEFMRLFGGSFTHQASVAKVVGKQGRGRAGIEASLDVEYLM SAGANISTWVYSSPGRHEAQEPFLQWLLLLSNESSLPHVHTVSYGDDEDSLSSIYIQRVNTEFMKAAARG LTLLFASGDTGAGCWSVSGRHKFRPSFPASSPYVTTVGGTSFKNPFLITDEVVDYISGGGFSNVFPRPPY QEEAVAQFLKSSSHLPPSSYFNASGRAYPDVAALSDGYWVVSNMVPIPWVSGTSASTPVFGGILSLINEH RILNGRPPLGFLNPRLYQQHGTGLFDVTHGCHESCLNEEVEGQGFCSGPGWDPVTGWGTPNFPALLKTLL NP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009906 |
| ORF Size | 1689 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009906.6 |
| RefSeq Size | 3494 bp |
| RefSeq ORF | 1689 bp |
| Locus ID | 12751 |
| UniProt ID | O89023 |
| MW | 61.3 kDa |
| Gene Summary | This gene encodes a lysosomal serine protease that cleaves N-terminal tripeptides from protein substrates. The encoded preproprotein undergoes autocatalytic processing to generate a mature enzyme. Mice lacking the encoded protein exhibit a progressive neurodegeneration and a greatly shortened lifespan. At the cellular level, mice lacking the encoded protein exhibit accumulation of autofluorescent lipopigments. Mutations in the human ortholog of this gene cause classical late-infantile neuronal ceroid lipofuscinosis. [provided by RefSeq, Nov 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Shelterin TPP1 Reduction Causes Telomere Attrition and Cellular Senescence via Sirt1 Deacetylase in Chronic Obstructive Pulmonary Disease
,Ahmad, T;Sundar, IK;Tormos, AM;Lerner, CA;Gerloff, J;Yao, H;Rahman, I;,
Am. J. Respir. Cell Mol. Biol.
,PubMed ID 27559927
[Tpp1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204227 | Tpp1 (untagged) - Mouse tripeptidyl peptidase I (Tpp1), (10ug) |
CNY 4,192.00 |
|
| MG208901 | Tpp1 (tGFP-tagged) - Mouse tripeptidyl peptidase I (Tpp1) |
CNY 5,510.00 |
|
| MR208901L3 | Lenti ORF clone of Tpp1 (Myc-DDK-tagged) - Mouse tripeptidyl peptidase I (Tpp1) |
CNY 6,940.00 |
|
| MR208901L4 | Lenti ORF clone of Tpp1 (mGFP-tagged) - Mouse tripeptidyl peptidase I (Tpp1) |
CNY 6,940.00 |
