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Npnt (NM_001029836) Mouse Tagged ORF Clone
CAT＃: MR208888
- TrueORF®
Npnt (Myc-DDK-tagged) - Mouse nephronectin (Npnt), transcript variant 2
ORF Plasmid: tGFP
"NM_001029836" in other vectors (4)
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CNY 4,184.00
CNY 4,940.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110009H02Rik; AA682063; AI314031; Nctn; POEM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208888 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGCTCCTAGCGGCGGTGCTCGCGTCCTCGCTCTACCTGCAGGTGGCTGCCGACTTCGACGGGG GGTGGCCCAGGCAAATAGTTTCTTCAATCGGCCTATGTCGCTATGGAGGCAGGATTGACTGCTGCTGGGG CTGGGCCCGCCAGTCCTGGGGACAGTGCCAACCTGTGTGTCAACCACAGTGCAAACACGGAGAGTGCGTC GGGCCAAACAAGTGCAAATGTCACCCTGGATTTGCTGGGAAAACCTGCAACCAAGATTTAAACGAGTGTG GCCTGAAGCCTCGGCCCTGTAAGCACAGGTGCATGAACACTTTCGGCAGCTACAAGTGCTACTGTCTCAA CGGATACATGCTGCTGCCAGACGGGTCCTGCTCAAGTGCCCTATCGTGTTCCATGGCAAACTGTCAGTAT GGCTGTGACGTGGTCAAAGGACAGGTCCGATGTCAGTGTCCATCCCCTGGCCTGCAGCTAGCTCCCGATG GGAGGACCTGTGTGGATATTGATGAATGTGCGACTGGAAGAGTCTCCTGCCCTCGATTTAGGCAGTGTGT CAACACGTTTGGGAGCTACATCTGCAAGTGTCACACTGGTTTCGACCTCATGTACATTGGAGGCAAATAT CAGTGCCATGACATCGACGAGTGCTCTCTTGGACAGCACCAGTGTAGCAGCTATGCCCGGTGTTACAACA TACATGGGTCCTACAAGTGCCAATGTAGAGATGGATACGAGGGGGATGGACTGAACTGTGTGTATATCCC CAAAGTCATGATTGAACCTTCAGGTCCAATCCATATGCCAGAAAGAAATGGTACAATCTCAAAGGGTGAT GGAGGACATGCGAATAGGATTCCTGATGCTGGAAGTACAAGGTGGCCCCTGAAGACACCATATATTCCTC CTGTCATTACCAACAGGCCTACTTCCAAGCCAACAACAAGACCTACACCAAACCCAACACCACAGCCTAC TCCACCACCTCCACCACCCCTCCCGACAGAGCCCAGAACAACTCCACTACCACCAACCCCAGAAAGGCCA TCTACCAGACCCACCACTATAGCACCTGCTACCAGTACCACTACACGAGTAATTACGGTTGACAACAGGA TACAGACGGATCCTCAGAAACCCAGAGGAGATGTGTTCATTCCACGGCAGCCGACAAATGACCTGTTTGA GATATTTGAAATCGAAAGAGGGGTCAGCGCGGATGAGGAAGTAAAGGACGACCCAGGTATTCTCATACAC AGCTGCAATTTTGACCATGGACTCTGTGGATGGATCAGAGAAAAAGATAGTGACTTGCACTGGGAGACAG CCAGGGACCCAGCAGGTGGACAGTATCTCACAGTGTCTGCAGCCAAAGCCCCGGGGGGAAAAGCCGCTCG CTTGGTGCTACGTCTCGGCCACCTCATGCATTCAGGGGACCTGTGCCTGTCCTTTAGGCACAAGGTGACT GGGCTGCACTCGGGCACACTGCAGGTGTTTGTGAGGAAACACGGTACCCACGGAGCAGCCCTGTGGGGAA GAAATGGTGGCCATGGCTGGAGGCAAACCCAGATCACCTTGCGAGGGGCTGACGTCAAAAGCGTCATCTT CAAAGGTGAAAAAAGGCGTGGTCACACGGGGGAGATTGGATTGGATGATGTGAGCTTGAAAAGAGGTCGC TGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208888 protein sequence
Red=Cloning site Green=Tags(s) MAVLLAAVLASSLYLQVAADFDGGWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCQPVCQPQCKHGECV GPNKCKCHPGFAGKTCNQDLNECGLKPRPCKHRCMNTFGSYKCYCLNGYMLLPDGSCSSALSCSMANCQY GCDVVKGQVRCQCPSPGLQLAPDGRTCVDIDECATGRVSCPRFRQCVNTFGSYICKCHTGFDLMYIGGKY QCHDIDECSLGQHQCSSYARCYNIHGSYKCQCRDGYEGDGLNCVYIPKVMIEPSGPIHMPERNGTISKGD GGHANRIPDAGSTRWPLKTPYIPPVITNRPTSKPTTRPTPNPTPQPTPPPPPPLPTEPRTTPLPPTPERP STRPTTIAPATSTTTRVITVDNRIQTDPQKPRGDVFIPRQPTNDLFEIFEIERGVSADEEVKDDPGILIH SCNFDHGLCGWIREKDSDLHWETARDPAGGQYLTVSAAKAPGGKAARLVLRLGHLMHSGDLCLSFRHKVT GLHSGTLQVFVRKHGTHGAALWGRNGGHGWRQTQITLRGADVKSVIFKGEKRRGHTGEIGLDDVSLKRGR C TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001029836 |
| ORF Size | 1686 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001029836.1, NM_001029836.2, NP_001025007.1 |
| RefSeq Size | 4619 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 114249 |
| UniProt ID | Q91V88 |
| MW | 61.4 kDa |
| Gene Summary | Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Phorbol-12-myristate 13-acetate inhibits Nephronectin gene expression via Protein kinase C alpha and c-Jun/c-Fos transcription factors
,null,
Scientific Reports
,PubMed ID 34645824
[Npnt]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218928 | Npnt (untagged) - Mouse nephronectin (Npnt), transcript variant 2, (10ug) |
CNY 7,130.00 |
|
| MG208888 | Npnt (tGFP-tagged) - Mouse nephronectin (Npnt), transcript variant 2 |
CNY 5,420.00 |
|
| MR208888L3 | Lenti ORF clone of Npnt (Myc-DDK-tagged) - Mouse nephronectin (Npnt), transcript variant 2 |
CNY 6,840.00 |
|
| MR208888L4 | Lenti ORF clone of Npnt (mGFP-tagged) - Mouse nephronectin (Npnt), transcript variant 2 |
CNY 6,840.00 |
