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Slc22a1 (NM_009202) Mouse Tagged ORF Clone
CAT＃: MR208809
- TrueORF®
Slc22a1 (Myc-DDK-tagged) - Mouse solute carrier family 22 (organic cation transporter), member 1 (Slc22a1)
ORF Plasmid: tGFP
"NM_009202" in other vectors (4)
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CNY 4,152.00
CNY 4,940.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Lx1; Oct1; Orct; Orct1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208809 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCACCGTGGACGATGTCCTGGAGCACGTTGGAGAGTTTGGCTGGTTCCAGAAGCAAGCCTTCCTGT TGCTATGCCTGATCTCAGCTTCTTTAGCTCCCATCTACGTGGGCATCGTTTTCCTGGGCTTCACCCCCGA CCACCACTGCCGGAGTCCTGGAGTGGCCGAGCTGAGCCAGCGGTGTGGCTGGAGCCCGGCAGAGGAGCTG AACTACACCGTGCCGGGCCTGGGGTCTGCGGGTGAGGCCTCCTTCCTCAGCCAATGCATGAAGTATGAGG TGGACTGGAACCAGAGCACCCTTGACTGTGTGGACCCACTGTCCAGCCTGGCTGCCAACAGGAGCCACTT GCCACTGAGCCCCTGCGAGCATGGCTGGGTGTACGACACTCCCGGCTCCTCCATCGTCACTGAGTTTAAC CTGGTGTGTGGAGACGCCTGGAAAGTGGACCTTTTTCAGTCCTGTGTGAACTTGGGCTTCTTCCTGGGCT CCCTGGTTGTGGGTTACATTGCAGACAGGTTTGGCCGTAAGCTCTGCCTCCTGGTGACCACTCTGGTCAC CTCCCTGTCTGGCGTGTTAACAGCGGTGGCCCCAGACTATACATCCATGTTGCTCTTTCGCCTTCTGCAA GGCATGGTCAGCAAAGGCAGCTGGGTGTCTGGCTACACCTTGATCACAGAGTTTGTCGGCTCTGGCTACA GGAGAACGACAGCCATCTTGTACCAGGTGGCCTTCACAGTGGGGCTAGTGGGGCTTGCTGGGGTGGCCTA TGCCATTCCAGACTGGCGCTGGCTCCAGCTGGCGGTGTCCCTGCCCACCTTCCTCTTCCTGCTGTATTAC TGGTTTGTCCCAGAATCCCCCCGGTGGCTGTTGTCTCAGAAGAGAACCACTCAAGCGGTAAGGATAATGG AGCAAATTGCACAGAAGAACAGGAAGGTGCCCCCTGCTGACCTGAAGATGATGTGCCTTGAGGAAGATGC CTCAGAGAGGCGGAGTCCTTCGTTTGCAGACCTGTTCCGCACCCCCAGCCTGAGGAAGCACACCCTCATC CTGATGTATCTATGGTTCTCTTGTGCTGTGCTGTACCAGGGCCTCATCATGCATGTGGGAGCCACAGGGG CCAACCTCTACCTGGACTTCTTTTATTCTTCTCTAGTGGAATTCCCTGCGGCCTTTATCATCCTTGTCAC CATTGACCGCATTGGCCGCATCTACCCAATAGCGGCATCAAATCTGGTGGCGGGGGCAGCCTGCCTCCTC ATGATCTTTATCCCACATGAGCTGCATTGGTTGAATGTGACCCTCGCTTGTCTTGGCCGTATGGGAGCCA CCATTGTGTTGCAGATGGTCTGCCTAGTGAACGCTGAGCTGTACCCTACATTCATCAGGAATCTCGGGAT GATGGTGTGCTCTGCCCTGTGTGACCTAGGTGGGATCTTCACCCCCTTCATGGTGTTCAGGCTGATGGAA GTTTGGCAAGCCCTGCCCCTCATTTTGTTTGGGGTTTTGGGCCTGAGTGCTGGGGCTGTGACCCTTCTAC TTCCGGAGACCAAGGGCGTGGCTCTGCCTGAGACTATTGAAGAAGCGGAGAACCTGGGAAGGAGGAAATC AAAGGCCAAAGAAAACACGATTTACCTTCAGGTCCAAACAGGCAAATCCCCACATACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208809 protein sequence
Red=Cloning site Green=Tags(s) MPTVDDVLEHVGEFGWFQKQAFLLLCLISASLAPIYVGIVFLGFTPDHHCRSPGVAELSQRCGWSPAEEL NYTVPGLGSAGEASFLSQCMKYEVDWNQSTLDCVDPLSSLAANRSHLPLSPCEHGWVYDTPGSSIVTEFN LVCGDAWKVDLFQSCVNLGFFLGSLVVGYIADRFGRKLCLLVTTLVTSLSGVLTAVAPDYTSMLLFRLLQ GMVSKGSWVSGYTLITEFVGSGYRRTTAILYQVAFTVGLVGLAGVAYAIPDWRWLQLAVSLPTFLFLLYY WFVPESPRWLLSQKRTTQAVRIMEQIAQKNRKVPPADLKMMCLEEDASERRSPSFADLFRTPSLRKHTLI LMYLWFSCAVLYQGLIMHVGATGANLYLDFFYSSLVEFPAAFIILVTIDRIGRIYPIAASNLVAGAACLL MIFIPHELHWLNVTLACLGRMGATIVLQMVCLVNAELYPTFIRNLGMMVCSALCDLGGIFTPFMVFRLME VWQALPLILFGVLGLSAGAVTLLLPETKGVALPETIEEAENLGRRKSKAKENTIYLQVQTGKSPHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009202 |
| ORF Size | 1671 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009202.5 |
| RefSeq Size | 1994 bp |
| RefSeq ORF | 1671 bp |
| Locus ID | 20517 |
| UniProt ID | O08966 |
| MW | 61.5 kDa |
| Gene Summary | Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Fine Mapping and Functional Analysis Reveal a Role of SLC22A1 in Acylcarnitine Transport
,Kim, HI;Raffler, J;Lu, W;Lee, J;Abbey, D;Saleheen, D;Rabinowitz, JD;Bennett, MJ;Hand, NJ;Brown, C;Rader, DJ;,
AJHG
[SLC22A1]
|
|
Sex-, Species-, and Tissue-Specific Metabolism of Empagliflozin in Male Mouse Kidney Forms an Unstable Hemiacetal Metabolite (M466/2) That Degrades to 4-Hydroxycrotonaldehyde, a Reactive and Cytotoxic Species
,Taub, ME;Ludwig-Schwellinger, E;Ishiguro, N;Kishimoto, W;Yu, H;Wagner, K;Tweedie, D;,
Chem. Res. Toxicol.
,PubMed ID 25489797
[SLC22A1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200831 | Slc22a1 (untagged) - Mouse solute carrier family 22 (organic cation transporter), member 1 (Slc22a1), (10ug) |
CNY 4,152.00 |
|
| MG208809 | Slc22a1 (tGFP-tagged) - Mouse solute carrier family 22 (organic cation transporter), member 1 (Slc22a1) |
CNY 5,420.00 |
|
| MR208809L3 | Lenti ORF clone of Slc22a1 (Myc-DDK-tagged) - Mouse solute carrier family 22 (organic cation transporter), member 1 (Slc22a1) |
CNY 6,840.00 |
|
| MR208809L4 | Lenti ORF clone of Slc22a1 (mGFP-tagged) - Mouse solute carrier family 22 (organic cation transporter), member 1 (Slc22a1) |
CNY 6,840.00 |
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