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Dtx1 (BC053055) Mouse Tagged ORF Clone
CAT＃: MR208731
- TrueORF®
Dtx1 (Myc-DDK-tagged) - Mouse deltex 1 homolog (Drosophila) (cDNA clone MGC:62339 IMAGE:5685525)
ORF Plasmid: tGFP
"BC053055" in other vectors (4)
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CNY 4,088.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fxit1; mKIAA4160 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208731 representing BC053055
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCAGTTCCGGCAAGACACAGGTACCATGCGGCCGGTGCGACGCAACTTCTACGATCCATCGTCGG CGCCGGGCAAGGGCATCGTGTGGGAATGGGAGAACGACGGCGGGGCGTGGACGGCCTACGACATGGACAT CTGCATCACCATCCAGAACGCGTACGAGAAGCAGCACCCGTGGCTCGACCTCTCATCGCTCGGCTTCTGC TACCTCATCTACTTCAACAGCATGTCCCAGATGAACCGCCAGACGCGCCGCCGCCGCCGCCTGCGCCGTC GCCTGGACCTGGCTTACCCGCTCACTGTCGGCTCCATTCCCAAGTCGCAATCCTGGCCCGTGGGAGCCAG CTCGGGTCAGCCCTGCTCCTGTCAGCAGTGCCTGCTGGTCAACAGCACGCGCGCCGCCTCCAACGCCATC CTGGCCTCGCAGCGCCGCAAGGCTCCCATTGCGCCAGCCGCGCCTCCAGCGCCCCCTCCGCCCCCGCCGC CGCTGCCACCCGGAGGACCTCCGGGTGCGCTCGTTGTGCGCCCCAGCGCCACTTTCGCCGGAGCTGCGCT CTGGGCCGCACCTGCCACCGGCCCCACGGAGCCTGCGCCGCCTCCAGGAGTTCCCCCAAGGAGCCCTAGT GCCCCCAACGGAGCGCCCACACCGGGCCAAAACAACCTCAGTCGACCAGGACCACAGAGGTCCACCAGCG TCAGCGCACGCGCCTCTATCCCGCCTGGGGTTCCGGCGCTCCCCGTGAAGAACTTGAATGGCACTGGCCC TGTCCACCCAGCCTTGGCAGGGATGACCGGGATCCTGCTGTGTGCAGCGGGGCTGCCGGTGTGCCTGACA CGAGCACCCAAACCCATCCTGCACCCACCACCAGTAAGCAAAAGCGACGTGAAGCCTGTGCCTGGAGTGC CCGGCGTGTGCCGCAAGACCAAGAAGAAACACCTCAAGAAAAGCAAGAATCCTGAGGATGTGGTTCGGAG GTACATGCAGAAGGTGAAAAACCCGCCTGATGAGGACTGTACCATTTGCATGGAGCGGCTGGTCACAGCA TCTGGCTATGAGGGCGTGCTCCGAAACAAGAGTGTGCGGCCCGAGCTTGTGGGCCGCCTGGGCCGCTGCG GCCACATGTATCACCTGCTCTGCCTGGTGGCCATGTACTCCAATGGCAACAAGGATGGCAGCCTGCAGTG TCCAACCTGCAAAGCCATCTACGGGGAGAAGACAGGGACACAGCCACCAGGGAAGATGGAGTTTCACCTC ATCCCGCACTCGCTGCCTGGTTTTGCAGACACCCAGACGATCCGCATCGTCTATGACATCCCCACGGGCA TCCAGGGCCCTGAACATCCCAACCCAGGCAAGAAGTTCACAGCCAGAGGCTTCCCTCGCCACTGCTACCT ACCCAACAATGAGAAGGGCCGAAAGGTGCTGAGATTGCTCATCACCGCCTGGGAACGCAGACTCATCTTC ACTATCGGAACATCCAACACCACGGGCGAGTCGGACACCGTGGTGTGGAACGAGATTCACCACAAGACGG AGTTTGGTTCCAACCTCACTGGTCACGGCTACCCCGACGCCAGCTACCTAGACAACGTGCTGGCTGAGCT CACCGCCCAGGGGGTTTCTGAGGCCATGGCCAAGGCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR208731 representing BC053055
Red=Cloning site Green=Tags(s) MHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWLDLSSLGFC YLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAI LASQRRKAPIAPAAPPAPPPPPPPLPPGGPPGALVVRPSATFAGAALWAAPATGPTEPAPPPGVPPRSPS APNGAPTPGQNNLSRPGPQRSTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLT RAPKPILHPPPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVTA SGYEGVLRNKSVRPELVGRLGRCGHMYHLLCLVAMYSNGNKDGSLQCPTCKAIYGEKTGTQPPGKMEFHL IPHSLPGFADTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPNNEKGRKVLRLLITAWERRLIF TIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASYLDNVLAELTAQGVSEAMAKA SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC053055 |
| ORF Size | 1647 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC053055.1 |
| RefSeq Size | 2882 bp |
| RefSeq ORF | 1649 bp |
| Locus ID | 14357 |
| MW | 105.6 kDa |
| Gene Summary | Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1. Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218169 | Dtx1 (untagged) - Mouse deltex 1 homolog (Drosophila) (cDNA clone MGC:62339 IMAGE:5685525), (10ug) |
CNY 6,940.00 |
|
| MG208731 | Dtx1 (tGFP-tagged) - Mouse deltex 1 homolog (Drosophila) (cDNA clone MGC:62339 IMAGE:5685525) |
CNY 5,688.00 |
|
| MR208731L3 | Lenti ORF clone of Dtx1 (Myc-DDK-tagged) - Mouse deltex 1 homolog (Drosophila) (cDNA clone MGC:62339 IMAGE:5685525) |
CNY 5,040.00 |
|
| MR208731L4 | Lenti ORF clone of Dtx1 (mGFP-tagged) - Mouse deltex 1 homolog (Drosophila) (cDNA clone MGC:62339 IMAGE:5685525) |
CNY 5,040.00 |
