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Nars (NM_027350) Mouse Tagged ORF Clone
CAT＃: MR208707
- TrueORF®
Nars (Myc-DDK-tagged) - Mouse asparaginyl-tRNA synthetase (Nars), transcript variant 2
ORF Plasmid: tGFP
"NM_027350" in other vectors (4)
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CNY 5,610.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 3010001M15Rik; AA960128; ASNRS; C78150 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208707 representing NM_027350
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCGGAGGTGATTAGGGGGACTGCAGAGATGGTTCTAGAGCTGTATGTATCTGACCGAGAAGGAA ATGACGCAACCGGGGATGGAACCAAGGAGAAACCATTTAAAACAGGCCTAAAGGCTTTGATGACAGTTGG AAAAGAGCCATTTCCCACCATTTATGTGGACTCACAAAAGGAAAATGAGAGATGGGATGTTATTTCTAAG TCACAGATGAAGAACATTAAAAAGATGTGGCACAGAGAACAGATGAAGAACGACTCTAGGGAGAAGAAAG AGGCAGAAGATAACTTACGAAGAGAAAAGAACCTGGAGGAAGCAAAAAAAATTATTATTAAAAATGACCC GAGCCTGCCAGAGCCGGCCTGTGTAAAGATTAGTGCATTAGAAGGATACAGAGGCCAGAGAGTGAAGGTG TTTGGCTGGGTCCACAGGTTGCGCAGGCAAGGAAAGAATTTGATGTTTTTGGTGTTGCGAGATGGTACGG GTTATCTTCAGTGTGTCTTGTCAGATGACTTGTGTCAGTGTTACAATGGAGTAGTCCTGTCCACCGAGAG TAGTGTGGCGGTGTACGGAACACTAAACCTAACTCCAAAGGGCAAACAGGCTCCAGGAGGCCATGAGCTG AGCTGTGACTTCTGGGAACTGGTGGGGCTGGCCCCGGCTGGAGGAGCTGATAACCTGATCAACGAGGAGT CTGATGTGGATGTCCAGCTCAACAACCGGCACATGATGATCCGGGGAGAGAACATGTCCAAAATCCTGAA AGCGCGCTCCATGATCACCAGGTGTTTCCGGGACCACTTCTTCGACAGGGGCTACTGTGAAGTTACCACT CCAACACTGGTGCAGACGCAGGTGGAAGGTGGGGCCACGCTCTTCAAGCTTGACTATTTCGGGGAAGAAG CGTTTTTGACCCAGTCCTCACAGTTGTACCTGGAGACCTGCCTTCCAGCCCTGGGAGATGTTTTTTGTAT AGCCCAGTCATACAGGGCCGAACAGTCCAGGACACGAAGGCACCTGGCTGAGTTCACTCACGTGGAAGCT GAGTGTCCTTTCCTGACCTTCGAGGACCTCCTGAACCGTTTAGAGGACCTAGTGTGTGATGTGGTGGACA GAGTCCTGAAGTCACCAGTGGCAAGCATAGTGTATGAGCTCAACCCGAACTTTAAACCCCCCAAGCGGCC TTTCCGCAGGATGAACTACTCGGATGCTATTGAGTGGCTGAAGGAGCATGATGTCAAGAAAGAAGACGGG ACTTTCTACGAGTTTGGAGATGATATTCCCGAAGCGCCTGAGAGACTGATGACAGACACCATTAATGAAC CAATCCTGCTGTGTCGCTTTCCTGTGGAGATCAAGTCTTTCTATATGCAGCGCTGTCCTGAGGATCCTCG ACTTACTGAATCTGTGGACGTGTTGATGCCCAACGTTGGTGAGATTGTAGGTGGCTCGATGCGCTCCTGG GACAGTGAGGAGATTCTCGAAGGCTATAAGAGGGAAGGGATTGACCCCGCTCCTTACTACTGGTATACAG ATCAGAGAAAATATGGCACCTGTCCTCATGGAGGGTATGGCTTGGGCTTGGAACGATTTCTTAGCTGGAT TCTGAACAGGTATCACATCCGAGATGTGTGCCTGTACCCTCGATTTCTCCAGCGCTGCAGGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208707 representing NM_027350
Red=Cloning site Green=Tags(s) MSSEVIRGTAEMVLELYVSDREGNDATGDGTKEKPFKTGLKALMTVGKEPFPTIYVDSQKENERWDVISK SQMKNIKKMWHREQMKNDSREKKEAEDNLRREKNLEEAKKIIIKNDPSLPEPACVKISALEGYRGQRVKV FGWVHRLRRQGKNLMFLVLRDGTGYLQCVLSDDLCQCYNGVVLSTESSVAVYGTLNLTPKGKQAPGGHEL SCDFWELVGLAPAGGADNLINEESDVDVQLNNRHMMIRGENMSKILKARSMITRCFRDHFFDRGYCEVTT PTLVQTQVEGGATLFKLDYFGEEAFLTQSSQLYLETCLPALGDVFCIAQSYRAEQSRTRRHLAEFTHVEA ECPFLTFEDLLNRLEDLVCDVVDRVLKSPVASIVYELNPNFKPPKRPFRRMNYSDAIEWLKEHDVKKEDG TFYEFGDDIPEAPERLMTDTINEPILLCRFPVEIKSFYMQRCPEDPRLTESVDVLMPNVGEIVGGSMRSW DSEEILEGYKREGIDPAPYYWYTDQRKYGTCPHGGYGLGLERFLSWILNRYHIRDVCLYPRFLQRCRP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027350 |
| ORF Size | 1674 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027350.3, NP_081626.2 |
| RefSeq Size | 2660 bp |
| RefSeq ORF | 1677 bp |
| Locus ID | 70223 |
| UniProt ID | Q8BP47 |
| MW | 64.7 kDa |
| Gene Summary | Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn). In addition to its essential role in protein synthesis, acts as a signaling molecule that induces immune response in a CCR3-dependent manner.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218924 | Nars (untagged) - Mouse asparaginyl-tRNA synthetase (Nars), transcript variant 2, (10ug) |
CNY 7,130.00 |
|
| MG208707 | Nars (tGFP-tagged) - Mouse asparaginyl-tRNA synthetase (Nars) |
CNY 6,180.00 |
|
| MR208707L3 | Lenti ORF clone of Nars (Myc-DDK-tagged) - Mouse asparaginyl-tRNA synthetase (Nars), transcript variant 2 |
CNY 7,510.00 |
|
| MR208707L4 | Lenti ORF clone of Nars (mGFP-tagged) - Mouse asparaginyl-tRNA synthetase (Nars), transcript variant 2 |
CNY 7,510.00 |
