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Dbr1 (BC006661) Mouse Tagged ORF Clone
CAT＃: MR208704
- TrueORF®
(Myc-DDK-tagged) - Mouse cDNA clone MGC:6384 IMAGE:3501390
ORF Plasmid: tGFP
"BC006661" in other vectors (3)
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CNY 4,072.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW018415 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208704 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGTGGCCGTGGCCGGCTGCTGCCACGGCGAGCTGGACAAGATCTACGAGACTCTGGCGCTGGCGG AGCGGCGCGGCTCGGGACCCGTGGATCTCCTTCTGTGCTGCGGCGACTTCCAGGCGGTGCGCAACGAGGC CGACCTGCGCTGCATGGCCGTGCCGCCCAAGTACCGTCACATGCAGACCTTCTACAGATATTACTCTGGA GAGAAAAAGGCCCCGGTTCTCACCATCTTCATTGGAGGAAACCATGAAGCCTCAAACCATTTGCAAGAGT TACCCTATGGTGGCTGGGTAGCGCCGAATATTTATTATTTAGGCTTGGCTGGTGTTGTCAAATACCGAGG AGTAAGGATTGGTGGCATCTCTGGCATATTTAAATCTCATGATTATAGAAAAGGTCATTTTGAGTGCCCC CCTTATAATTCATCTACCATAAGGAGTATATATCATGTGAGAAATATAGAGGTCTACAAATTAAAACAGT TAAAGCAGCCTGTACATATATTCTTGTCTCATGATTGGCCAAGAAATATATATCATTATGGCAATAAGAA GCAGCTTCTTAAGACCAAATCTTTTTTCCGCCAAGAAGTGGAAAACAGCACCCTGGGGAGCCCCGCTGCC TCGGAGCTGCTGGAGCACCTACAGCCTGCGTACTGGTTCTCCGCGCACCTGCATGTCAAGTTCGCAGCCC TGATGCAGCATCAGGCCACAGATAAAGGCCGAGCAGGCAAAGAAACCAAGTTTTTAGCCTTGGACAAGTG CTTGCCACACAGAGACTTCCTTCAGGTGTTAGAGATAGAACATGACCCCAGTGCTCCTGAGTACTTAGAG TATGACGTTGAATGGCTTACTGTTCTCAGGGCCACTGATGACCTGATTAATGTGACTGGGGGCCTGTGGA ATATGCCTGAAGACAACGGTCTGCACACAAGGTGGGATTATAGTGCAACGGAAGAAACTATGAAGGAAGT AATGGAAAAATTGAACCATGACCCTAAAGTCCCCTGTAACTTCACCATGACAGCTGCTTGTTATGATCCC AGTAAGCCCCAGACACAAGTGAAGCTGGTTCACAGGATCAATCCACAGACGACTGAGTTCTGTGCCCAGC TTGGCATCACAGACATCAATGTCATGATTCAGAAGGCCAGAGAGGAAGAGCACCATCAGTGTGGTGAATA TGAGCAGCAGGGTGACCCGGGGACTGAGGAGTCTGAAGAGGACCGGAGTGAGTACAACACGGACACGTCG GCCCTCTCCTCCATTAATCCAGATGAAATCATGCTGGATGAAGAGGAGGAGGAGGAGGAGGAGGCTGTAA GCGCTCACAGTGACATGAACACACCTTCTGTGGAACCTGCTTCTGACCAGGCTTCTGACCTCTCTACAAG CTTTTCTGACGTCAGGAACTTACCAAGTTCCATGTTTGTGTCCTCTGACGATGCATCCCGTTCTCCAGCA AGTGGAGAGGGGAAATGTGGTGAAACGGTGGAGTCTGGGGATGAAAAAGACTTGGCTAAGTTTCCTCTGA AGCGGCTGAGTGATGAACACGAACCTGAACAAAGGAAGAAAATAAAGAGGAGGAACCAAGCCATCTACGC TGCAGTGGATGATGGGGATGCTAGTGCCGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208704 protein sequence
Red=Cloning site Green=Tags(s) MRVAVAGCCHGELDKIYETLALAERRGSGPVDLLLCCGDFQAVRNEADLRCMAVPPKYRHMQTFYRYYSG EKKAPVLTIFIGGNHEASNHLQELPYGGWVAPNIYYLGLAGVVKYRGVRIGGISGIFKSHDYRKGHFECP PYNSSTIRSIYHVRNIEVYKLKQLKQPVHIFLSHDWPRNIYHYGNKKQLLKTKSFFRQEVENSTLGSPAA SELLEHLQPAYWFSAHLHVKFAALMQHQATDKGRAGKETKFLALDKCLPHRDFLQVLEIEHDPSAPEYLE YDVEWLTVLRATDDLINVTGGLWNMPEDNGLHTRWDYSATEETMKEVMEKLNHDPKVPCNFTMTAACYDP SKPQTQVKLVHRINPQTTEFCAQLGITDINVMIQKAREEEHHQCGEYEQQGDPGTEESEEDRSEYNTDTS ALSSINPDEIMLDEEEEEEEEAVSAHSDMNTPSVEPASDQASDLSTSFSDVRNLPSSMFVSSDDASRSPA SGEGKCGETVESGDEKDLAKFPLKRLSDEHEPEQRKKIKRRNQAIYAAVDDGDASAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC006661 |
| ORF Size | 1641 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC006661, AAH06661 |
| RefSeq Size | 2221 bp |
| RefSeq ORF | 1643 bp |
| Locus ID | 83703 |
| MW | 61.9 kDa |
| Gene Summary | Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208704 | (tGFP-tagged) - Mouse cDNA clone MGC:6384 IMAGE:3501390 |
CNY 3,420.00 |
|
| MR208704L3 | Lenti ORF clone of MGC:6384 (Myc-DDK-tagged) - Mouse cDNA clone MGC:6384 IMAGE:3501390 |
CNY 5,040.00 |
|
| MR208704L4 | Lenti ORF clone of MGC:6384 (mGFP-tagged) - Mouse cDNA clone MGC:6384 IMAGE:3501390 |
CNY 5,040.00 |
