Osbp2 (BC058356) Mouse Tagged ORF Clone

CAT#: MR208672

  • TrueORF®

Osbp2 (Myc-DDK-tagged) - Mouse oxysterol binding protein 2 (cDNA clone MGC:66677 IMAGE:5696357)

ORF Plasmid: DDK tGFP



  "BC058356" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,048.00

CNY 5,420.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms ORP-4, OSBPL1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208672 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCAACGCCTGTAGGGACTTCCTGGAACTAGCTGAGACTCACAGCCGGAAATGGCAGCGGGCTCTAA
ATTATGAGCAGGAGCAGCGTGTCCACCTGGAAGAGACAATTGAGCAGTTGGCCAAGCAGCACAACAGTCT
TGAACGGGCCTTCTGCAACACCCCTGGCGGGCCAGCCAGCTCCAGCAAGAGCTTCAGCGAGGGAAGCTTC
TTGACTTCCAAAGGAGAGAACAGTGAGGAAGATGAAGATACTGAGTACTTTGACGCCATGGAAGACTCTA
CATCTTTCATCACCGTAGTCACTGAGGCCAAGGAAGACAGAAAGCCTGAGAGTGGACCTGGGACCACCAC
TGTGGACTGGACCTCAGCAGATAATGTATTAGATGGTGCCTCATTCATGCCCAAGAATTCATGCAAAGTT
AAGAGGCGAGTCCGCATCCCTGACAAACCCAACTATAGCCTTAACCTCTGGAGCATCATGAAGAATTGTA
TTGGCCGAGAGCTTTCCCGGATCCCCATGCCGGTGAACTTCAATGAGCCCCTGTCCATGCTCCAGCGACT
TACAGAGGACCTGGAGTACCACCACCTGCTGGACAAGGCGGTGAACTGCACCAGCTCGGTGGAGCAGATG
TGCCTGGTAGCCGCCTTTTCTGTGTCCTCCTACTCCACCACGGTGCACCGCATCGCCAAGCCCTTCAACC
CTATGCTCGGGGAGACCTTCGAGCTGGACCGTATGGAGGACATGGGCCTGCGTTCCCTCTGTGAGCAGGT
GAGCCACCACCCCCCGTCTGCTGCCCACCACGTGTTCTCTAAGCATGGCTGGAGCCTCTGGCAAGAAATC
ACCATCGCCAGCAAGTTCCGAGGGAAATACATCTCTATCATGCCACTCGGTGCCATCCACCTAGAATTCC
AGGCCAGTGGCAATCACTACGTGTGGAGGAAGAGCACCTCCACCGTGCACAACATCATCGTGGGCAAGCT
CTGGATTGACCAGTCAGGGGACATTGAGATTGTGAACCACAAGACCAAGGACCGGTGCCAGCTGAAGTTC
GTACCCTACAGCTACTTCTCCAAAGAGGCAGCCCGAAAGGTGACTGGAGTGGTGAGTGACAGCCAGGGCA
AGGCCCACTACGTGCTGTCAGGTTCGTGGGATGACCAGATGGAATGTTCTAAGATTGTGCACAGCAGCCC
CAGCTCTGATGGGAGACAGAAAACCGTGTACCAGACACTGCCCGCCAAACTGCTCTGGAGGAAATACCCA
CTGCCGGAGAATGCGGAGAACATGTACTATTTCTCCGAGCTAGCCCTGACCCTCAACGAGCAGGAGGACG
GCGTGGCGCCCACCGACAGTCGCCTGCGGCCAGACCAGCGGCTTATGGAGAGGGGACGCTGGGACGAGGC
CAACACCGAGAAGCAACGGCTGGAGGAGAAGCAGCGCCTGTCCAGGCGACGGCGGCTGGAGTCATGCACG
GCAGGCTGCGGTGGGGAGGAAGAGAAGGAGTCAGATGGCTATGTGCCGCTCTGGTTCGAGAAGAGGCTGG
ACCCGCTGACTGGGGAGATGGCCTGCATGTACAAGGGCGGCTACTGGGAGGCCAAGGAGAAGAAGGACTG
GCACATGTGCCCCAACATCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208672 protein sequence
Red=Cloning site Green=Tags(s)

MINACRDFLELAETHSRKWQRALNYEQEQRVHLEETIEQLAKQHNSLERAFCNTPGGPASSSKSFSEGSF
LTSKGENSEEDEDTEYFDAMEDSTSFITVVTEAKEDRKPESGPGTTTVDWTSADNVLDGASFMPKNSCKV
KRRVRIPDKPNYSLNLWSIMKNCIGRELSRIPMPVNFNEPLSMLQRLTEDLEYHHLLDKAVNCTSSVEQM
CLVAAFSVSSYSTTVHRIAKPFNPMLGETFELDRMEDMGLRSLCEQVSHHPPSAAHHVFSKHGWSLWQEI
TIASKFRGKYISIMPLGAIHLEFQASGNHYVWRKSTSTVHNIIVGKLWIDQSGDIEIVNHKTKDRCQLKF
VPYSYFSKEAARKVTGVVSDSQGKAHYVLSGSWDDQMECSKIVHSSPSSDGRQKTVYQTLPAKLLWRKYP
LPENAENMYYFSELALTLNEQEDGVAPTDSRLRPDQRLMERGRWDEANTEKQRLEEKQRLSRRRRLESCT
AGCGGEEEKESDGYVPLWFEKRLDPLTGEMACMYKGGYWEAKEKKDWHMCPNIF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC058356
ORF Size 1632 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC058356, AAH58356
RefSeq Size 3281 bp
RefSeq ORF 1634 bp
Locus ID 74309
MW 62.4 kDa
Gene Summary The protein encoded by this gene belongs to the oxysterol-binding protein-related family of proteins, which are defined by a C-terminal sterol domain with a highly conserved EQVSHHPP motif. Oxysterols are oxygenated derivatives of cholesterol that are involved in mechanisms that include apoptosis, cholesterol homeostasis, lipid trafficking and cell differentiation. This protein is selectively expressed at high levels in the brain and testis. Within the testis, the mRNA is localized to postmeiotic germ cells, including spermatids and spermatozoa, but is not detectable in somatic cells. Mice homozygous mutant for a targeted deletion in this gene do not exhibit overt developmental phenotypes but are male sterile. Females display normal fertility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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