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Osbp2 (BC058356) Mouse Tagged ORF Clone
CAT＃: MR208672
- TrueORF®
Osbp2 (Myc-DDK-tagged) - Mouse oxysterol binding protein 2 (cDNA clone MGC:66677 IMAGE:5696357)
ORF Plasmid: tGFP
"BC058356" in other vectors (4)
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CNY 4,048.00
CNY 5,420.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ORP-4, OSBPL1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208672 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAACGCCTGTAGGGACTTCCTGGAACTAGCTGAGACTCACAGCCGGAAATGGCAGCGGGCTCTAA ATTATGAGCAGGAGCAGCGTGTCCACCTGGAAGAGACAATTGAGCAGTTGGCCAAGCAGCACAACAGTCT TGAACGGGCCTTCTGCAACACCCCTGGCGGGCCAGCCAGCTCCAGCAAGAGCTTCAGCGAGGGAAGCTTC TTGACTTCCAAAGGAGAGAACAGTGAGGAAGATGAAGATACTGAGTACTTTGACGCCATGGAAGACTCTA CATCTTTCATCACCGTAGTCACTGAGGCCAAGGAAGACAGAAAGCCTGAGAGTGGACCTGGGACCACCAC TGTGGACTGGACCTCAGCAGATAATGTATTAGATGGTGCCTCATTCATGCCCAAGAATTCATGCAAAGTT AAGAGGCGAGTCCGCATCCCTGACAAACCCAACTATAGCCTTAACCTCTGGAGCATCATGAAGAATTGTA TTGGCCGAGAGCTTTCCCGGATCCCCATGCCGGTGAACTTCAATGAGCCCCTGTCCATGCTCCAGCGACT TACAGAGGACCTGGAGTACCACCACCTGCTGGACAAGGCGGTGAACTGCACCAGCTCGGTGGAGCAGATG TGCCTGGTAGCCGCCTTTTCTGTGTCCTCCTACTCCACCACGGTGCACCGCATCGCCAAGCCCTTCAACC CTATGCTCGGGGAGACCTTCGAGCTGGACCGTATGGAGGACATGGGCCTGCGTTCCCTCTGTGAGCAGGT GAGCCACCACCCCCCGTCTGCTGCCCACCACGTGTTCTCTAAGCATGGCTGGAGCCTCTGGCAAGAAATC ACCATCGCCAGCAAGTTCCGAGGGAAATACATCTCTATCATGCCACTCGGTGCCATCCACCTAGAATTCC AGGCCAGTGGCAATCACTACGTGTGGAGGAAGAGCACCTCCACCGTGCACAACATCATCGTGGGCAAGCT CTGGATTGACCAGTCAGGGGACATTGAGATTGTGAACCACAAGACCAAGGACCGGTGCCAGCTGAAGTTC GTACCCTACAGCTACTTCTCCAAAGAGGCAGCCCGAAAGGTGACTGGAGTGGTGAGTGACAGCCAGGGCA AGGCCCACTACGTGCTGTCAGGTTCGTGGGATGACCAGATGGAATGTTCTAAGATTGTGCACAGCAGCCC CAGCTCTGATGGGAGACAGAAAACCGTGTACCAGACACTGCCCGCCAAACTGCTCTGGAGGAAATACCCA CTGCCGGAGAATGCGGAGAACATGTACTATTTCTCCGAGCTAGCCCTGACCCTCAACGAGCAGGAGGACG GCGTGGCGCCCACCGACAGTCGCCTGCGGCCAGACCAGCGGCTTATGGAGAGGGGACGCTGGGACGAGGC CAACACCGAGAAGCAACGGCTGGAGGAGAAGCAGCGCCTGTCCAGGCGACGGCGGCTGGAGTCATGCACG GCAGGCTGCGGTGGGGAGGAAGAGAAGGAGTCAGATGGCTATGTGCCGCTCTGGTTCGAGAAGAGGCTGG ACCCGCTGACTGGGGAGATGGCCTGCATGTACAAGGGCGGCTACTGGGAGGCCAAGGAGAAGAAGGACTG GCACATGTGCCCCAACATCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208672 protein sequence
Red=Cloning site Green=Tags(s) MINACRDFLELAETHSRKWQRALNYEQEQRVHLEETIEQLAKQHNSLERAFCNTPGGPASSSKSFSEGSF LTSKGENSEEDEDTEYFDAMEDSTSFITVVTEAKEDRKPESGPGTTTVDWTSADNVLDGASFMPKNSCKV KRRVRIPDKPNYSLNLWSIMKNCIGRELSRIPMPVNFNEPLSMLQRLTEDLEYHHLLDKAVNCTSSVEQM CLVAAFSVSSYSTTVHRIAKPFNPMLGETFELDRMEDMGLRSLCEQVSHHPPSAAHHVFSKHGWSLWQEI TIASKFRGKYISIMPLGAIHLEFQASGNHYVWRKSTSTVHNIIVGKLWIDQSGDIEIVNHKTKDRCQLKF VPYSYFSKEAARKVTGVVSDSQGKAHYVLSGSWDDQMECSKIVHSSPSSDGRQKTVYQTLPAKLLWRKYP LPENAENMYYFSELALTLNEQEDGVAPTDSRLRPDQRLMERGRWDEANTEKQRLEEKQRLSRRRRLESCT AGCGGEEEKESDGYVPLWFEKRLDPLTGEMACMYKGGYWEAKEKKDWHMCPNIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC058356 |
| ORF Size | 1632 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC058356, AAH58356 |
| RefSeq Size | 3281 bp |
| RefSeq ORF | 1634 bp |
| Locus ID | 74309 |
| MW | 62.4 kDa |
| Gene Summary | The protein encoded by this gene belongs to the oxysterol-binding protein-related family of proteins, which are defined by a C-terminal sterol domain with a highly conserved EQVSHHPP motif. Oxysterols are oxygenated derivatives of cholesterol that are involved in mechanisms that include apoptosis, cholesterol homeostasis, lipid trafficking and cell differentiation. This protein is selectively expressed at high levels in the brain and testis. Within the testis, the mRNA is localized to postmeiotic germ cells, including spermatids and spermatozoa, but is not detectable in somatic cells. Mice homozygous mutant for a targeted deletion in this gene do not exhibit overt developmental phenotypes but are male sterile. Females display normal fertility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217906 | Osbp2 (untagged) - Mouse oxysterol binding protein 2 (cDNA clone MGC:66677 IMAGE:5696357), (10ug) |
CNY 6,940.00 |
|
| MG208672 | Osbp2 (tGFP-tagged) - Mouse oxysterol binding protein 2 (cDNA clone MGC:66677 IMAGE:5696357) |
CNY 5,990.00 |
|
| MR208672L3 | Lenti ORF clone of Osbp2 (Myc-DDK-tagged) - Mouse oxysterol binding protein 2 (cDNA clone MGC:66677 IMAGE:5696357) |
CNY 7,320.00 |
|
| MR208672L4 | Lenti ORF clone of Osbp2 (mGFP-tagged) - Mouse oxysterol binding protein 2 (cDNA clone MGC:66677 IMAGE:5696357) |
CNY 7,320.00 |
