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Mfsd2a (NM_029662) Mouse Tagged ORF Clone
CAT＃: MR208553
- TrueORF®
Mfsd2a (Myc-DDK-tagged) - Mouse major facilitator superfamily domain containing 2A (Mfsd2a)
ORF Plasmid: tGFP
"NM_029662" in other vectors (3)
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CNY 5,976.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1700018O18Rik; Mfsd2; NLS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208553 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAAGGAGAAGGCGCCGAGAGCGGTTCCGCGGCGGGGCTGCTCCCCACCAGCATCCTCCAAGCCA GTGAACGGCCGGTCCAGGTGAAGAAGGAACCAAAAAAGAAGCAGCAACTGTCCATTTGCAACAAGCTTTG CTATGCAGTTGGAGGGGCCCCGTACCAGTTGACCGGCTGCGCACTGGGATTCTTCCTGCATATCTACCTA TTGGATGTGGCTAAGGTGGAACCACTTCCTGCTTCCATTATCCTTTTTGTGGGCCGAGCCTGGGATGCCT TCACTGACCCTCTGGTGGGCTTCTGCATTAGCAAGTCCTCCTGGACCCGCCTGGGCCGCCTCATGCCCTG GATCATCTTCTCCACTCCCCTGGCCATCATTGCTTACTTCCTCATCTGGTTTGTGCCTGACTTCCCATCA GGGACGGAAAGTTCACACGGCTTCCTTTGGTACCTGCTTTTCTATTGCCTCTTTGAGACACTGGTCACGT GCTTTCATGTTCCCTACTCAGCGCTCACCATGTTCATCAGCACGGAGCAGAGTGAGCGTGACTCAGCCAC GGCATACAGAATGACTGTGGAGGTGCTGGGCACAGTGATAGGCACAGCGATTCAAGGACAAATTGTGGGC CAAGCCAAGGCACCTTGTCTCCAGGACCAGAATGGCTCTGTGGTGGTCTCAGAAGTTGCCAATCGCACCC AGAGTACTGCCTCCCTCAAAGACACGCAAAATGCTTACCTGCTGGCAGCAGGGATCATCGCCTCCATCTA CGTCCTCTGTGCCTTCATTCTGATCCTAGGCGTGCGGGAGCAGAGAGAACTCTACGAGTCCCAGCAGGCT GAGTCAATGCCCTTCTTTCAGGGCCTCCGGCTGGTCATGGGTCATGGCCCCTATGTCAAGCTCATTGCCG GCTTCCTTTTTACCTCCCTGGCTTTCATGCTGGTGGAGGGTAACTTTGCCTTGTTCTGCACCTATACCTT GGACTTCCGAAATGAGTTCCAGAACCTCCTCCTGGCCATCATGCTCTCGGCCACATTCACCATCCCTATC TGGCAGTGGTTCCTAACCCGGTTTGGCAAGAAGACAGCTGTATACATCGGGATCTCTTCTGCAGTTCCTT TTCTCATCTTGGTGGCCCTCATGGAGCGTAATCTAATCGTCACTTACGTGGTGGCCGTAGCAGCTGGCGT CAGTGTAGCAGCTGCCTTCCTACTACCATGGTCCATGCTGCCTGACGTTATCGATGACTTCCACCTGAAA CACCCTCACTCCCCTGGCACCGAGCCCATATTCTTCTCCTTCTATGTCTTCTTCACCAAGTTTGCCTCTG GAGTCTCACTGGGTGTCTCTACCCTCAGTCTCGACTTTGCCAACTACCAGAGGCAGGGATGCTCCCAGCC AGAACAGGTCAAGTTTACACTGAAGATGCTGGTGACCATGGCTCCTATCATCCTCATCTTGCTGGGCCTG CTGCTCTTCAAGCTCTACCCCATTGATGAGGAGAAGCGGCGACAGAATAAGAAAGCTCTGCAGGCTCTAC GAGAAGAAGCCAGCAGCTCAGGTTGCTCGGATACAGACTCCACAGAGCTGGCCAGTATTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208553 protein sequence
Red=Cloning site Green=Tags(s) MAKGEGAESGSAAGLLPTSILQASERPVQVKKEPKKKQQLSICNKLCYAVGGAPYQLTGCALGFFLHIYL LDVAKVEPLPASIILFVGRAWDAFTDPLVGFCISKSSWTRLGRLMPWIIFSTPLAIIAYFLIWFVPDFPS GTESSHGFLWYLLFYCLFETLVTCFHVPYSALTMFISTEQSERDSATAYRMTVEVLGTVIGTAIQGQIVG QAKAPCLQDQNGSVVVSEVANRTQSTASLKDTQNAYLLAAGIIASIYVLCAFILILGVREQRELYESQQA ESMPFFQGLRLVMGHGPYVKLIAGFLFTSLAFMLVEGNFALFCTYTLDFRNEFQNLLLAIMLSATFTIPI WQWFLTRFGKKTAVYIGISSAVPFLILVALMERNLIVTYVVAVAAGVSVAAAFLLPWSMLPDVIDDFHLK HPHSPGTEPIFFSFYVFFTKFASGVSLGVSTLSLDFANYQRQGCSQPEQVKFTLKMLVTMAPIILILLGL LLFKLYPIDEEKRRQNKKALQALREEASSSGCSDTDSTELASIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029662 |
| ORF Size | 1605 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029662.1, NP_083938.1 |
| RefSeq Size | 2166 bp |
| RefSeq ORF | 1605 bp |
| Locus ID | 76574 |
| UniProt ID | Q9DA75 |
| MW | 59 kDa |
| Gene Summary | Sodium-dependent lysophosphatidylcholine (LPC) symporter, which plays an essential role for blood-brain barrier formation and function (PubMed:24828044, PubMed:24828040). Specifically expressed in endothelium of the blood-brain barrier of micro-vessels and transports LPC into the brain. Transport of LPC is essential because it constitutes the major mechanism by which docosahexaenoic acid (DHA), an omega-3 fatty acid that is essential for normal brain growth and cognitive function, enters the brain. Transports LPC carrying long-chain fatty acids such LPC oleate and LPC palmitate with a minimum acyl chain length of 14 carbons. Does not transport docosahexaenoic acid in unesterified fatty acid (PubMed:24828044). Specifically required for blood-brain barrier formation and function, probably by mediating lipid transport. Not required for central nervous system vascular morphogenesis (PubMed:24828040). Acts as a transporter for tunicamycin, an inhibitor of asparagine-linked glycosylation.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208553 | Mfsd2a (tGFP-tagged) - Mouse major facilitator superfamily domain containing 2 (Mfsd2) |
CNY 7,576.00 |
|
| MR208553L3 | Lenti ORF clone of Mfsd2a (Myc-DDK-tagged) - Mouse major facilitator superfamily domain containing 2A (Mfsd2a) |
CNY 7,220.00 |
|
| MR208553L4 | Lenti ORF clone of Mfsd2a (mGFP-tagged) - Mouse major facilitator superfamily domain containing 2A (Mfsd2a) |
CNY 8,376.00 |
