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Mutyh (NM_133250) Mouse Tagged ORF Clone
CAT＃: MR208268
- TrueORF®
Mutyh (Myc-DDK-tagged) - Mouse mutY homolog (E. coli) (Mutyh), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid: tGFP
"NM_133250" in other vectors (4)
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CNY 3,832.00
CNY 4,470.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5730495A01Rik; Mutyha; Mutyhalpha; Mutyhb; Mutyhbeta; Mutyhc; Myh |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208268 representing NM_133250
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAACTCCAAGCATCTGTGAGGAGTCACAAAAAGCAACCAGCCAACCACAAGAGAAGGAGAACAC GTGCCCTTAGCAGCAGCCAGGCCAAGCCTTCCAGCCTGGATGGCCTGGCCAAGCAGAAGAGAGAGGAGTT ACTGCAGGCCTCTGTCTCCCCATATCATCTCTTCAGCGACGTGGCCGATGTCACAGCTTTCCGAAGCAAC TTGCTCAGCTGGTATGACCAAGAGAAGCGTGACCTCCCTTGGAGAAACTTGGCAAAAGAAGAGGCCAACT CGGACAGGAGGGCCTATGCTGTGTGGGTGTCAGAGGTTATGCTGCAGCAGACCCAGGTTGCCACAGTGAT CGACTATTATACTCGATGGATGCAGAAGTGGCCAAAGCTTCAAGACCTGGCCAGCGCTTCCCTGGAGGAG GTGAACCAGCTCTGGTCTGGCCTGGGCTACTATTCTCGAGGCCGTCGGCTACAAGAAGGAGCTAGGAAGG TGGTAGAGGAGCTAGGAGGCCATATGCCACGTACAGCAGAGACCCTACAGCAGCTCCTGCCTGGTGTGGG GCGGTACACGGCTGGAGCCATTGCTTCCATCGCCTTTGACCAGGTAACCGGTGTGGTGGATGGGAACGTT TTACGGGTGCTGTGCCGTGTCCGGGCCATTGGTGCCGATCCCACCAGCACCCTTGTCTCTCATCACCTCT GGAACTTAGCCCAGCAGCTCGTGGACCCAGCCCGGCCTGGGGACTTCAATCAAGCTGCCATGGAGCTGGG GGCTACAGTGTGCACCCCACAGCGCCCTCTCTGCAGCCATTGCCCCGTGCAGAGTTTATGCCGGGCATAC CAGAGGGTGCAGCGGGGTCAGCTCTCAGCCTTGCCAGGCCGTCCTGACATAGAGGAGTGTGCTCTCAACA CTAGACAGTGCCAGCTTTGCCTCACTTCCTCAAGCCCCTGGGACCCCAGCATGGGAGTGGCCAACTTTCC TCGAAAGGCCAGCCGCAGGCCTCCCAGGGAGGAGTACTCTGCCACCTGTGTTGTGGAGCAGCCCGGAGCC ATCGGGGGTCCCCTGGTCCTCCTGGTGCAAAGGCCTGACTCAGGTCTGCTGGCAGGACTGTGGGAGTTCC CATCTGTCACCTTGGAGCCCTCAGAACAGCATCAGCACAAAGCCCTTCTGCAGGAACTGCAGCGCTGGTG TGGGCCCCTCCCTGCCATTCGTCTCCAGCACCTTGGGGAGGTTATCCACATCTTCTCTCACATCAAACTG ACGTATCAAGTATATAGTCTGGCTCTAGATCAAGCCCCAGCGAGCACTGCACCTCCTGGCGCTCGATGGC TGACCTGGGAGGAATTCTGCAATGCAGCTGTGTCTACTGCCATGAAAAAGGTGTTCCGCATGTATGAGGA CCATCGGCAAGGGACCCGCAAGGGTTCAAAAAGGTCCCAGGTGTGTCCTCCATCAAGCAGGAAAAAACCC AGCCTGGGACAGCAAGTCCTGGATACTTTCTTTCAGCGTCATATCCCCACAGACAAACCCAACAGTACTA CCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208268 representing NM_133250
Red=Cloning site Green=Tags(s) MKKLQASVRSHKKQPANHKRRRTRALSSSQAKPSSLDGLAKQKREELLQASVSPYHLFSDVADVTAFRSN LLSWYDQEKRDLPWRNLAKEEANSDRRAYAVWVSEVMLQQTQVATVIDYYTRWMQKWPKLQDLASASLEE VNQLWSGLGYYSRGRRLQEGARKVVEELGGHMPRTAETLQQLLPGVGRYTAGAIASIAFDQVTGVVDGNV LRVLCRVRAIGADPTSTLVSHHLWNLAQQLVDPARPGDFNQAAMELGATVCTPQRPLCSHCPVQSLCRAY QRVQRGQLSALPGRPDIEECALNTRQCQLCLTSSSPWDPSMGVANFPRKASRRPPREEYSATCVVEQPGA IGGPLVLLVQRPDSGLLAGLWEFPSVTLEPSEQHQHKALLQELQRWCGPLPAIRLQHLGEVIHIFSHIKL TYQVYSLALDQAPASTAPPGARWLTWEEFCNAAVSTAMKKVFRMYEDHRQGTRKGSKRSQVCPPSSRKKP SLGQQVLDTFFQRHIPTDKPNSTTQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133250 |
| ORF Size | 1545 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133250.2, NP_573513.2 |
| RefSeq Size | 1643 bp |
| RefSeq ORF | 1548 bp |
| Locus ID | 70603 |
| UniProt ID | Q99P21 |
| MW | 57.7 kDa |
| Gene Summary | Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Base-excision repair deficiency alone or combined with increased oxidative stress does not increase mtDNA point mutations in mice
,null,
Nucleic Acids Research
,PubMed ID 29860357
[Mutyh]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203318 | Mutyh (untagged) - Mouse mutY homolog (E. coli) (Mutyh), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug) |
CNY 2,000.00 |
|
| MG208268 | Mutyh (tGFP-tagged) - Mouse mutY homolog (E. coli) (Mutyh) |
CNY 4,940.00 |
|
| MR208268L3 | Lenti ORF clone of Mutyh (Myc-DDK-tagged) - Mouse mutY homolog (E. coli) (Mutyh), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 6,370.00 |
|
| MR208268L4 | Lenti ORF clone of Mutyh (mGFP-tagged) - Mouse mutY homolog (E. coli) (Mutyh), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 6,370.00 |
