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Cttn (BC011434) Mouse Tagged ORF Clone
CAT＃: MR208186
- TrueORF®
Cttn (Myc-DDK-tagged) - Mouse cortactin (cDNA clone MGC:18474 IMAGE:3981559)
ORF Plasmid: tGFP
"BC011434" in other vectors (4)
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CNY 5,040.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110020L01Rik; Ems1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208186 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAAAGCCTCTGCAGGCCATGCTGTGTCCATCACGCAGGATGATGGAGGAGCTGATGACTGGGAGA CTGATCCTGATTTTGTGAATGATGTGAGTGAAAAGGAGCAGAGATGGGGTGCTAAAACCGTGCAGGGATC GGGGCACCAGGAACACATCAACATTCACAAGCTTCGAGAGAATGTCTTCCAAGAACACCAGACGCTCAAG GAGAAGGAGCTGGAAACGGGACCCAAGGCTTCCCACGGCTATGGCGGGAAGTTCGGTGTGGAGCAGGATA GGATGGACAGATCAGCCGTGGGCCATGAGTACCAGTCGAAGCTTTCCAAGCACTGCTCACAAGTGGACTC GGTCCGGGGCTTCGGAGGCAAGTTCGGTGTCCAGATGGACAGGGTGGATCAGTCTGCTGTAGGCTTTGAA TACCAGGGGAAGACTGAGAAGCATGCCTCCCAGAAAGACTACTCTAGTGGCTTCGGTGGCAAATACGGTG TGCAAGCTGACCGTGTAGACAAGAGTGCCGTGGGCTTTGACTACCAGGGCAAGACGGAGAAGCATGAGTC TCAGAAAGATTACTCCAAAGGTTTTGGTGGCAAATATGGGATTGACAAGGACAAGGTGGATAAAAGTGCT GTGGGCTTTGAGTATCAAGGCAAGACAGAGAAGCACGAATCCCAGAAAGACTATGTAAAAGGCTTTGGAG GAAAGTTTGGTGTGCAGACAGACAGACAGGACAAGTGTGCCCTTGGCTGGGACCATCAGGAGAAGCTGCA GCTGCATGAATCCCAAAAAGACTATGCCAAAGGATTCGGCGGGAAGTATGGGGTGCAGAAGGATCGGATG GACAAGAATGCATCCACCTTTGAAGAAGTGGTCCAGGTGCCATCTGCCTATCAGAAGACTGTCCCCATTG AGGCCGTAACCAGCAAAACCAGTAATATCCGTGCTAACTTTGAAAACCTGGCAAAGGAGAGAGAGCAGGA GGACAGGCGGAAGGCAGAAGCCGAGAGAGCTCAGCGGATGGCCAAAGAAAGACAGGAGCAGGAGGAGGCG CGCAGGAAGCTGGAAGAGCAAGCCAGAGCCAAGAAGCAGACGCCCCCTGCATCCCCTAGTCCTCAACCAA TTGAAGACAGACCACCCTCCAGCCCCATCTATGAGGATGCAGCTCCGTTCAAGGCCGAGCCGAGCTACCG AGGTAGCGAACCTGAGCCTGAGTACAGCATCGAGGCCGCAGGCATTCCTGAGGCTGGCAGCCAGCAAGGC CTGACCTATACATCAGAGCCCGTGTACGAGACTACAGAGGCTCCTGGCCACTATCAAGCAGAGGATGACA CCTACGATGGGTATGAGAGTGACCTGGGCATCACAGCCATCGCCCTGTATGACTACCAGGCTGCTGGCGA TGATGAGATCTCCTTTGACCCTGATGACATCATCACCAACATAGAAATGATTGACGATGGCTGGTGGCGT GGGGTGTGCAAGGGCAGATACGGGCTCTTCCCAGCCAACTATGTGGAGCTGCGGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208186 protein sequence
Red=Cloning site Green=Tags(s) MWKASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLK EKELETGPKASHGYGGKFGVEQDRMDRSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFE YQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQKDYSKGFGGKYGIDKDKVDKSA VGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYAKGFGGKYGVQKDRM DKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQRMAKERQEQEEA RRKLEEQARAKKQTPPASPSPQPIEDRPPSSPIYEDAAPFKAEPSYRGSEPEPEYSIEAAGIPEAGSQQG LTYTSEPVYETTEAPGHYQAEDDTYDGYESDLGITAIALYDYQAAGDDEISFDPDDIITNIEMIDDGWWR GVCKGRYGLFPANYVELRQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC011434 |
| ORF Size | 1527 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC011434, AAH11434 |
| RefSeq Size | 2916 bp |
| RefSeq ORF | 1529 bp |
| Locus ID | 13043 |
| MW | 57.1 kDa |
| Gene Summary | Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:17403031). Plays a role in the formation of lamellipodia and in cell migration (By similarity). Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (PubMed:22262902). Plays a role in the invasiveness of cancer cells, and the formation of metastases (By similarity). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (By similarity). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218158 | Cttn (untagged) - Mouse cortactin (cDNA clone MGC:18474 IMAGE:3981559), (10ug) |
CNY 6,940.00 |
|
| MG208186 | Cttn (tGFP-tagged) - Mouse cortactin (cDNA clone MGC:18474 IMAGE:3981559) |
CNY 5,392.00 |
|
| MR208186L3 | Lenti ORF clone of Cttn (Myc-DDK-tagged) - Mouse cortactin (cDNA clone MGC:18474 IMAGE:3981559) |
CNY 6,940.00 |
|
| MR208186L4 | Lenti ORF clone of Cttn (mGFP-tagged) - Mouse cortactin (cDNA clone MGC:18474 IMAGE:3981559) |
CNY 6,940.00 |
