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Dkc1 (NM_001030307) Mouse Tagged ORF Clone
CAT＃: MR208185
- TrueORF®
Dkc1 (Myc-DDK-tagged) - Mouse dyskeratosis congenita 1, dyskerin homolog (human) (Dkc1)
ORF Plasmid: tGFP
"NM_001030307" in other vectors (4)
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CNY 6,064.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BC068171 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208185 representing NM_001030307
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATGCAGAAGTAATTACATTCCCAAAGAAGCATAAGAAGAAAAAAGACCGGAAGCCATTACAAG AAGATGATGTTGCCGAAATACAACATGCTGAAGAATTTCTTATCAAACCAGAATCTAAAGTGGCTCAATT AGACACTTCTCAGTGGCCCTTGTTACTAAAGAATTTTGATAAGCTAAATGTAAGGACAGCACACTATACA CCTCTTCCTTGTGGTTCAAATCCTCTGAAGAGGGAGATTGGGGACTATATCAGGACCGGTTTCATTAATC TTGACAAGCCCTCTAACCCCTCTTCCCATGAGGTGGTAGCCTGGATCCGACGAATACTTCGGGTAGAGAA GACTGGCCACAGTGGCACACTAGATCCCAAAGTGACTGGTTGTTTAATTGTGTGCATTGAACGAGCCACT CGTTTGGTGAAATCACAACAGAGTGCAGGCAAAGAGTATGTTGGAATTGTCCGGCTGCACAATGCTATTG AAGGGGGTACTCAGCTTTCTAGGGCCCTAGAAACTCTGACAGGTGCCCTGTTTCAGCGACCCCCACTTAT TGCTGCAGTAAAGAGGCAGCTTCGAGTGAGGACTATCTACGAGAGCAAAATGATAGAATATGATCCTGAG AGAAGATTAGGAATCTTTTGGGTAAGCTGTGAAGCTGGCACCTACATTCGGACACTATGCGTACACCTTG GCTTGTTACTGGGAGTTGGTGGTCAGATGCAGGAACTTCGGAGGGTGCGTTCTGGAGTCATGAGTGAAAA GGACCACATGGTGACAATGCATGATGTACTCGATGCTCAGTGGCTGTATGATAACCATAAGGATGAGAGT TACTTGCGGCGTGTTGTTTACCCTTTGGAAAAGCTGTTGACATCTCATAAACGTCTGGTTATGAAAGATA GTGCAGTGAATGCAATCTGCTATGGGGCCAAGATCATGCTTCCTGGTCTTCTTCGATATGAAGATGGCAT TGAGGTCAACCAGGAGATTGTGGTCATTACTACTAAGGGGGAAGCTATCTGCATGGCAATTGCATTGATG ACTACAGCAGTGATTTCTACCTGTGACCATGGTATAGTAGCCAAAATAAAGAGGGTGATCATGGAGAGAG ACACTTATCCTCGCAAGTGGGGTTTAGGTCCAAAGGCAAGTCAGAAGAAGATGATGATCAAGCAGGGCCT TCTGGACAAGCATGGCAAACCCACAGACAATACCCCTGCCACATGGAAACAGGATTACATTGACTATAGT GATTCTGGCAAGAACACACTGGTTACCGAAGCAGTACAAGCCCCTCAGTTAGCTGCTGAAGCAGTAAATG TCATAAAAAGGAAGCGGGATAGTGAGAGTGAAAGTGATGAGACCCCAACAGTTCCCCAGTTGAAGGAAAA GAAGAAGAAGAAGGATAAGAAGCCCAAAACTGTGCTAGAAAGTGGGGGCGAAACTGGAGATGGGGACAAC GACACCACCAAAAAGAAAAAGAAGAAGAAAGTAAAAGTGGTAGAAGAGATGTCTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208185 representing NM_001030307
Red=Cloning site Green=Tags(s) MADAEVITFPKKHKKKKDRKPLQEDDVAEIQHAEEFLIKPESKVAQLDTSQWPLLLKNFDKLNVRTAHYT PLPCGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVEKTGHSGTLDPKVTGCLIVCIERAT RLVKSQQSAGKEYVGIVRLHNAIEGGTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPE RRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDES YLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGLLRYEDGIEVNQEIVVITTKGEAICMAIALM TTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKMMIKQGLLDKHGKPTDNTPATWKQDYIDYS DSGKNTLVTEAVQAPQLAAEAVNVIKRKRDSESESDETPTVPQLKEKKKKKDKKPKTVLESGGETGDGDN DTTKKKKKKKVKVVEEMSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001030307 |
| ORF Size | 1527 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001030307.2, NP_001025478.1 |
| RefSeq Size | 2803 bp |
| RefSeq ORF | 1530 bp |
| Locus ID | 245474 |
| UniProt ID | Q9ESX5 |
| MW | 57.9 kDa |
| Gene Summary | Catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA (PubMed:12522253, PubMed:15240872). This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs (PubMed:12522253, PubMed:15240872). Required for ribosome biogenesis and telomere maintenance (By similarity). Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217214 | Dkc1 (untagged) - Mouse dyskeratosis congenita 1, dyskerin homolog (human) (Dkc1), (10ug) |
CNY 6,560.00 |
|
| MG208185 | Dkc1 (tGFP-tagged) - Mouse dyskeratosis congenita 1, dyskerin homolog (human) (Dkc1) |
CNY 5,510.00 |
|
| MR208185L3 | Lenti ORF clone of Dkc1 (Myc-DDK-tagged) - Mouse dyskeratosis congenita 1, dyskerin homolog (human) (Dkc1) |
CNY 6,940.00 |
|
| MR208185L4 | Lenti ORF clone of Dkc1 (mGFP-tagged) - Mouse dyskeratosis congenita 1, dyskerin homolog (human) (Dkc1) |
CNY 6,940.00 |
