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Ranbp10 (BC024698) Mouse Tagged ORF Clone
CAT＃: MR208076
- TrueORF®
Ranbp10 (Myc-DDK-tagged) - Mouse RAN binding protein 10 (cDNA clone MGC:30446 IMAGE:3667000)
ORF Plasmid: tGFP
"BC024698" in other vectors (3)
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CNY 3,744.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4432417N03Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208076 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAATTGGACTCTCAGCGCAAGGCGTTAACATGAACAGACTGCCTGGTTGGGACAAGCATTCCTATG GTTACCATGGCGATGATGGGCATTCCTTCTGCTCCTCTGGGACAGGCCAGCCTTATGGTCCCACATTCAC CACAGGGGATGTGATCGGCTGTTGTGTCAACCTCATCAATGGCACCTGCTTCTACACCAAGAATGGCCAC AGCCTTGGTATCGCCTTCACAGATCTCCCGGCCAACCTCTACCCCACTGTCGGCCTACAGACACCTGGGG AGATTGTGGATGCCAACTTTGGGCAGCAGCCCTTCCTGTTCGACATTGAGGACTATATGCGGGAGTGGCG TGCCAAAGTCCAGGGCACTGTGCACGGCTTTCCCATAAGTGCCCGGCTTGGCGAGTGGCAGGCGGTGCTG CAGAACATGGTCTCGTCTTACCTGGTGCATCATGGGTATTGTTCCACAGCCACAGCTTTTGCCCGAATGA CTGAAACCCCGATTCAGGAAGAGCAGGCATCCATAAAGAACAGACAAAAAATCCAGAAGCTGGTGCTGGA AGGCCGGGTGGGTGAAGCCATTGAGACAACCCAGCGCTTCTACCCTGGGCTGCTGGAACATAATCCCAAC CTGCTCTTCATGCTCAAGTGTCGACAGTTCGTGGAGATGGTGAACGGCACTGACAGTGAGGTCCGCAGCC TGAGTTCCCGAAGCCCCAAGTCCCAGGACAGCTACCCTGGCTCCCCCAGCCTCAGCCCCCGACATGGCCC CAGTAGTTCCCATATACACAACACAGGAGCAGACAGTCCCAGCTGCAGCAATGGCGTCGCATCCACCAAG AACAAACAGAACCACAGTAAATACCCCGCACCCAGCTCCTCCTCCTCGTCCTCATCCTCCTCGTCCTCCT CTTCCCCGTCCTCCGTCAATTACTCCGAGTCCAACTCAACAGACTCTACCAAGTCCCAGCCCCACAGCAG TACCAGTAACCAGGAGACCAGTGATAGCGAGATGGAGATGGAGGCAGAGCACTATCCCAATGGTGTGCTG GAAAGCGTCTCCACGCGCATCGTCAATGGGGCCTATAAGCATGACGACCTGCAGACAGATGAGTCCAGCA TGGACGACGGGCATCCACGGAGGCAGCTCTGCGGGGGCAACCAGGCTGCCACAGAAAGGATCATCCTGTT TGGCCGAGAGCTGCAGGCATTGAGCGAGCAGCTGGGCCGGGAGTACGGCAAGAACTTGGCCCACACGGAG ATGCTGCAGGATGCCTTTAGCCTACTAGCATACTCAGACCCCTGGAGCTGCCCAGTAGGTCATCAGCTTG ATCCCATCCAGAGAGAGCCCGTGTGTGCTGCTCTCAACAGCGCCATTTTAGAGTCTCAGAACCTGCCAAA GCAGCCTCCTTTGATGCTCGCCCTGGGCCAGGCATCTGAATGTCTACGGCTCATGGCCCGAGCAGGCCTG GGATCTTGCTCCTTTGCCAGAGTCGATGACTACTTGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208076 protein sequence
Red=Cloning site Green=Tags(s) MGIGLSAQGVNMNRLPGWDKHSYGYHGDDGHSFCSSGTGQPYGPTFTTGDVIGCCVNLINGTCFYTKNGH SLGIAFTDLPANLYPTVGLQTPGEIVDANFGQQPFLFDIEDYMREWRAKVQGTVHGFPISARLGEWQAVL QNMVSSYLVHHGYCSTATAFARMTETPIQEEQASIKNRQKIQKLVLEGRVGEAIETTQRFYPGLLEHNPN LLFMLKCRQFVEMVNGTDSEVRSLSSRSPKSQDSYPGSPSLSPRHGPSSSHIHNTGADSPSCSNGVASTK NKQNHSKYPAPSSSSSSSSSSSSSSPSSVNYSESNSTDSTKSQPHSSTSNQETSDSEMEMEAEHYPNGVL ESVSTRIVNGAYKHDDLQTDESSMDDGHPRRQLCGGNQAATERIILFGRELQALSEQLGREYGKNLAHTE MLQDAFSLLAYSDPWSCPVGHQLDPIQREPVCAALNSAILESQNLPKQPPLMLALGQASECLRLMARAGL GSCSFARVDDYLH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC024698 |
| ORF Size | 1509 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC024698, AAH24698 |
| RefSeq Size | 5169 bp |
| RefSeq ORF | 1511 bp |
| Locus ID | 74334 |
| MW | 54.7 kDa |
| Gene Summary | May act as an adapter protein to couple membrane receptors to intracellular signaling pathways (Probable). Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (By similarity). Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation (By similarity). Acts as a guanine nucleotide exchange factor (GEF) for RAN GTPase (PubMed:18347012). May play an essential role in hemostasis and in maintaining microtubule dynamics with respect to both platelet shape and function (PubMed:19801445).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217908 | Ranbp10 (untagged) - Mouse RAN binding protein 10 (cDNA clone MGC:30446 IMAGE:3667000), (10ug) |
CNY 6,940.00 |
|
| MG208076 | Ranbp10 (tGFP-tagged) - Mouse RAN binding protein 10 (cDNA clone MGC:30446 IMAGE:3667000) |
CNY 3,140.00 |
|
| MR208076L3 | Lenti ORF clone of Ranbp10 (Myc-DDK-tagged) - Mouse RAN binding protein 10 (cDNA clone MGC:30446 IMAGE:3667000) |
CNY 4,750.00 |
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