United States
Germany
Japan
United Kingdom
China
Blk (NM_007549) Mouse Tagged ORF Clone
CAT＃: MR208004
- TrueORF®
Blk (Myc-DDK-tagged) - Mouse B lymphoid kinase (Blk)
ORF Plasmid: tGFP
"NM_007549" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208004 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGCTGAGCAGCAAGAGGCAGGTCAGTGAGAAGGGCAAGGGCTGGAGCCCCGTGAAGATCCGCA CCCAGGACAAGGCTCCCCCACCCCTGCCACCCCTGGTTGTCTTCAACCACCTTGCCCCACCATCTCCTAA CCAGGACCCAGATGAAGAGGAGCGTTTTGTGGTGGCTCTGTTTGACTATGCCGCTGTGAATGACAGGGAC CTTCAGGTGCTGAAGGGTGAGAAGCTCCAGGTCTTGAGGAGCACTGGAGACTGGTGGTTGGCCAGGTCAC TCGTCACAGGAAGAGAAGGTTATGTGCCCAGCAACTTTGTGGCCCCAGTAGAGACTCTGGAAGTAGAAAA ATGGTTCTTCAGGACCATCAGCCGGAAGGATGCTGAGAGGCAGTTGCTGGCTCCGATGAACAAGGCCGGC TCCTTTCTCATCAGAGAGAGTGAGAGCAATAAAGGTGCCTTTTCCCTGTCCGTGAAAGATATCACCACCC AGGGGGAGGTGGTCAAGCACTATAAGATCCGATCACTGGACAATGGAGGCTATTACATCTCCCCCCGGAT CACCTTTCCCACCCTCCAGGCCCTGGTGCAGCACTATTCAAAGAAAGGGGATGGTTTGTGTCAGAAGTTG ACTCTGCCCTGTGTGAACCTGGCCCCGAAGAACCTTTGGGCCCAAGATGAATGGGAAATCCCCAGGCAGT CTCTCAAGTTGGTCCGGAAACTTGGGTCTGGGCAGTTTGGCGAAGTCTGGATGGGTTATTACAAAAATAA CATGAAGGTGGCCATCAAGACCCTGAAGGAGGGAACCATGTCACCGGAAGCTTTCCTGGGCGAGGCCAAC GTGATGAAAACCCTGCAGCATGAGAGGCTGGTTCGTCTCTACGCTGTGGTCACCAGAGAGCCCATTTACA TCGTCACTGAATACATGGCCAGAGGATGCTTGCTGGATTTTCTGAAGACCGATGAAGGTAGCAGGTTGTC CCTTCCAAGGCTGATTGACATGTCAGCCCAGGTTGCAGAGGGGATGGCTTACATAGAGCGCATGAATTCC ATCCACCGTGACCTGCGGGCAGCCAACATCCTGGTGTCTGAGACGTTGTGCTGCAAAATCGCTGACTTCG GCTTGGCCAGGATCATTGACAGTGAATACACTGCCCAAGAGGGGGCCAAGTTCCCCATCAAGTGGACCGC CCCGGAGGCCATCCACTTCGGGGTGTTTACCATCAAGGCTGATGTGTGGTCCTTCGGAGTCTTGCTGATG GAGATTGTCACCTATGGGCGCGTTCCCTACCCAGGAATGAGCAACCCTGAGGTCATCCGTAGCCTGGAGC ACGGCTACCGAATGCCATGCCCGGAGACATGTCCACCGGAGTTGTACAATGATATCATCACTGAGTGCTG GCGGGGCCGGCCAGAGGAGCGGCCTACCTTTGAGTTCCTGCAGTCGGTGTTGGAGGACTTCTACACAGCC ACGGAGGGCCAATATGAGCTGCAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208004 protein sequence
Red=Cloning site Green=Tags(s) MGLLSSKRQVSEKGKGWSPVKIRTQDKAPPPLPPLVVFNHLAPPSPNQDPDEEERFVVALFDYAAVNDRD LQVLKGEKLQVLRSTGDWWLARSLVTGREGYVPSNFVAPVETLEVEKWFFRTISRKDAERQLLAPMNKAG SFLIRESESNKGAFSLSVKDITTQGEVVKHYKIRSLDNGGYYISPRITFPTLQALVQHYSKKGDGLCQKL TLPCVNLAPKNLWAQDEWEIPRQSLKLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEAN VMKTLQHERLVRLYAVVTREPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQVAEGMAYIERMNS IHRDLRAANILVSETLCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWSFGVLLM EIVTYGRVPYPGMSNPEVIRSLEHGYRMPCPETCPPELYNDIITECWRGRPEERPTFEFLQSVLEDFYTA TEGQYELQP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007549 |
| ORF Size | 1500 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007549.2, NP_031575.2 |
| RefSeq Size | 2475 bp |
| RefSeq ORF | 1500 bp |
| Locus ID | 12143 |
| UniProt ID | P16277 |
| MW | 56.7 kDa |
| Gene Summary | Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors (PubMed:2404338, PubMed:7690139, PubMed:7608542, PubMed:9636152, PubMed:14662906, PubMed:12563261). Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation (PubMed:2404338, PubMed:7690139, PubMed:7608542, PubMed:14662906, PubMed:12563261). Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor (PubMed:2404338, PubMed:7690139, PubMed:7608542, PubMed:14662906, PubMed:12563261). Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207' (PubMed:7592958, PubMed:9177269). Phosphorylates also the immunoglobulin G receptor FCGR2 (By similarity). With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation (PubMed:14662906, PubMed:12563261). Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation (PubMed:7565679). In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose (By similarity). Phosphorylates CGAS, promoting retention of CGAS in the cytosol (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216930 | Blk (untagged) - Mouse B lymphoid kinase (Blk), (10ug) |
CNY 6,460.00 |
|
| MG208004 | Blk (tGFP-tagged) - Mouse B lymphoid kinase (Blk) |
CNY 3,710.00 |
|
| MR208004L3 | Lenti ORF clone of Blk (Myc-DDK-tagged) - Mouse B lymphoid kinase (Blk) |
CNY 5,230.00 |
|
| MR208004L4 | Lenti ORF clone of Blk (mGFP-tagged) - Mouse B lymphoid kinase (Blk) |
CNY 5,230.00 |
