Prcp (BC055022) Mouse Tagged ORF Clone
CAT#: MR207866
- TrueORF®
Prcp (Myc-DDK-tagged) - Mouse prolylcarboxypeptidase (angiotensinase C) (cDNA clone MGC:62717 IMAGE:4017462)
ORF Plasmid: tGFP
"BC055022" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PCP, HUMPCP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207866 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCCGGGCTCTCCTGCTCCTGTCTTTCCTATTACTCGGGGCCGCCACAACCATCCCGCCTAGGC TAAAGACCCTCGGCAGCCCGCATTTGTCAGCCAGTCCCACACCCGACCCTGCTGTAGCCCGTAAATACTC AGTTCTTTACTTCGAACAGAAGGTTGACCACTTTGGGTTTGCTGACATGAGAACTTTTAAACAGCGGTAC CTAGTCGCTGATAAACACTGGCAGAGAAATGGTGGATCAATACTTTTCTACACTGGTAACGAAGGGGATA TTGTCTGGTTTTGCAATAATACGGGGTTCATGTGGGATGTGGCTGAGGAGCTGAAAGCTATGCTGGTGTT CGCTGAGCATCGATACTATGGGGAGTCCCTTCCCTTTGGTCAGGACTCCTTCAAGGATTCCCAACACTTG AATTTCCTGACATCAGAACAGGCTCTGGCTGATTTTGCAGAGTTAATCAGACACTTGGAAAAAACAATCC CAGGAGCTCAAGGTCAACCTGTCATCGCCATCGGGGGCTCTTATGGTGGCATGCTTGCAGCCTGGTTCAG GATGAAATATCCTCACATTGTAGTTGGAGCTCTTGCAGCTTCTGCCCCTATCTGGCAGCTTGATGGTATG GTTCCCTGTGGTGAGTTTATGAAGATTGTAACTAACGATTTCAGGAAAAGTGGTCCTTATTGTTCAGAAA GTATCCGCAAATCCTGGAATGTCATTGACAAACTCTCAGGTTCAGGCAGTGGTCTCCAGTCGCTCACGAA CATTCTTCACCTATGTAGTCCCTTGACTTCTGAGAAAATCCCAACTTTAAAAGGATGGATAGCTGAGACC TGGGTGAACCTGGCCATGGTGAACTACCCTTACGCATGCAACTTTTTGCAGCCTTTGCCTGCTTGGCCCA TCAAGGAGGTGTGCCAATATTTGAAAAATCCTAATGTATCGGATACAGTGTTGCTGCAGAATATTTTCCA AGCTCTGAGTGTATATTATAACTATTCAGGCCAAGCAGCATGCCTGAATATTTCACAAACGACTACGAGC AGTCTGGGCTCCATGGGCTGGAGCTTTCAGGCCTGCACAGAAATGGTCATGCCCTTTTGTACTAATGGTA TTGATGACATGTTTGAACCCTTTCTGTGGGACTTGGAGAAGTATTCCAATGACTGTTTTAACCAGTGGGG AGTGAAACCACGGCCTCATTGGATGACTACCATGTACGGTGGCAAAAACATCAGTTCACACTCAAACATC ATTTTCAGCAATGGTAAGTTAGACCCGTGGTCAGGAGGCGGAGTAACGAGAGACATCACAGACACCCTGG TGGCTATCAACATCCCTGACGGGGCCCATCATTTAGATCTCCGAGCCCACAATGCCTTCGATCCTTCCTC TGTGCTGTTATCTCGATTACTGGAAGTTAAACACATGAAGAAGTGGATTTTAGATTTCTATAGCAATATC CAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207866 protein sequence
Red=Cloning site Green=Tags(s) MGCRALLLLSFLLLGAATTIPPRLKTLGSPHLSASPTPDPAVARKYSVLYFEQKVDHFGFADMRTFKQRY LVADKHWQRNGGSILFYTGNEGDIVWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFGQDSFKDSQHL NFLTSEQALADFAELIRHLEKTIPGAQGQPVIAIGGSYGGMLAAWFRMKYPHIVVGALAASAPIWQLDGM VPCGEFMKIVTNDFRKSGPYCSESIRKSWNVIDKLSGSGSGLQSLTNILHLCSPLTSEKIPTLKGWIAET WVNLAMVNYPYACNFLQPLPAWPIKEVCQYLKNPNVSDTVLLQNIFQALSVYYNYSGQAACLNISQTTTS SLGSMGWSFQACTEMVMPFCTNGIDDMFEPFLWDLEKYSNDCFNQWGVKPRPHWMTTMYGGKNISSHSNI IFSNGKLDPWSGGGVTRDITDTLVAINIPDGAHHLDLRAHNAFDPSSVLLSRLLEVKHMKKWILDFYSNI Q TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC055022 |
ORF Size | 1473 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC055022, AAH55022 |
RefSeq Size | 2339 bp |
RefSeq ORF | 1475 bp |
Locus ID | 72461 |
MW | 55 kDa |
Gene Summary | Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218448 | Prcp (untagged) - Mouse prolylcarboxypeptidase (angiotensinase C) (cDNA clone MGC:62717 IMAGE:4017462), (10ug) |
CNY 6,940.00 |
|
MG207866 | Prcp (tGFP-tagged) - Mouse prolylcarboxypeptidase (angiotensinase C) (cDNA clone MGC:62717 IMAGE:4017462) |
CNY 3,040.00 |
|
MR207866L3 | Lenti ORF clone of Prcp (Myc-DDK-tagged) - Mouse prolylcarboxypeptidase (angiotensinase C) (cDNA clone MGC:62717 IMAGE:4017462) |
CNY 4,660.00 |
|
MR207866L4 | Lenti ORF clone of Prcp (mGFP-tagged) - Mouse prolylcarboxypeptidase (angiotensinase C) (cDNA clone MGC:62717 IMAGE:4017462) |
CNY 4,660.00 |