Prcp (BC055022) Mouse Tagged ORF Clone

CAT#: MG207866

  • TrueORF®

Prcp (tGFP-tagged) - Mouse prolylcarboxypeptidase (angiotensinase C) (cDNA clone MGC:62717 IMAGE:4017462)

ORF Plasmid: DDK tGFP



  "BC055022" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,040.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms PCP, HUMPCP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207866 representing BC055022
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTGCCGGGCTCTCCTGCTCCTGTCTTTCCTATTACTCGGGGCCGCCACAACCATCCCGCCTAGGC
TAAAGACCCTCGGCAGCCCGCATTTGTCAGCCAGTCCCACACCCGACCCTGCTGTAGCCCGTAAATACTC
AGTTCTTTACTTCGAACAGAAGGTTGACCACTTTGGGTTTGCTGACATGAGAACTTTTAAACAGCGGTAC
CTAGTCGCTGATAAACACTGGCAGAGAAATGGTGGATCAATACTTTTCTACACTGGTAACGAAGGGGATA
TTGTCTGGTTTTGCAATAATACGGGGTTCATGTGGGATGTGGCTGAGGAGCTGAAAGCTATGCTGGTGTT
CGCTGAGCATCGATACTATGGGGAGTCCCTTCCCTTTGGTCAGGACTCCTTCAAGGATTCCCAACACTTG
AATTTCCTGACATCAGAACAGGCTCTGGCTGATTTTGCAGAGTTAATCAGACACTTGGAAAAAACAATCC
CAGGAGCTCAAGGTCAACCTGTCATCGCCATCGGGGGCTCTTATGGTGGCATGCTTGCAGCCTGGTTCAG
GATGAAATATCCTCACATTGTAGTTGGAGCTCTTGCAGCTTCTGCCCCTATCTGGCAGCTTGATGGTATG
GTTCCCTGTGGTGAGTTTATGAAGATTGTAACTAACGATTTCAGGAAAAGTGGTCCTTATTGTTCAGAAA
GTATCCGCAAATCCTGGAATGTCATTGACAAACTCTCAGGTTCAGGCAGTGGTCTCCAGTCGCTCACGAA
CATTCTTCACCTATGTAGTCCCTTGACTTCTGAGAAAATCCCAACTTTAAAAGGATGGATAGCTGAGACC
TGGGTGAACCTGGCCATGGTGAACTACCCTTACGCATGCAACTTTTTGCAGCCTTTGCCTGCTTGGCCCA
TCAAGGAGGTGTGCCAATATTTGAAAAATCCTAATGTATCGGATACAGTGTTGCTGCAGAATATTTTCCA
AGCTCTGAGTGTATATTATAACTATTCAGGCCAAGCAGCATGCCTGAATATTTCACAAACGACTACGAGC
AGTCTGGGCTCCATGGGCTGGAGCTTTCAGGCCTGCACAGAAATGGTCATGCCCTTTTGTACTAATGGTA
TTGATGACATGTTTGAACCCTTTCTGTGGGACTTGGAGAAGTATTCCAATGACTGTTTTAACCAGTGGGG
AGTGAAACCACGGCCTCATTGGATGACTACCATGTACGGTGGCAAAAACATCAGTTCACACTCAAACATC
ATTTTCAGCAATGGTAAGTTAGACCCGTGGTCAGGAGGCGGAGTAACGAGAGACATCACAGACACCCTGG
TGGCTATCAACATCCCTGACGGGGCCCATCATTTAGATCTCCGAGCCCACAATGCCTTCGATCCTTCCTC
TGTGCTGTTATCTCGATTACTGGAAGTTAAACACATGAAGAAGTGGATTTTAGATTTCTATAGCAATATC
CAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207866 representing BC055022
Red=Cloning site Green=Tags(s)

MGCRALLLLSFLLLGAATTIPPRLKTLGSPHLSASPTPDPAVARKYSVLYFEQKVDHFGFADMRTFKQRY
LVADKHWQRNGGSILFYTGNEGDIVWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFGQDSFKDSQHL
NFLTSEQALADFAELIRHLEKTIPGAQGQPVIAIGGSYGGMLAAWFRMKYPHIVVGALAASAPIWQLDGM
VPCGEFMKIVTNDFRKSGPYCSESIRKSWNVIDKLSGSGSGLQSLTNILHLCSPLTSEKIPTLKGWIAET
WVNLAMVNYPYACNFLQPLPAWPIKEVCQYLKNPNVSDTVLLQNIFQALSVYYNYSGQAACLNISQTTTS
SLGSMGWSFQACTEMVMPFCTNGIDDMFEPFLWDLEKYSNDCFNQWGVKPRPHWMTTMYGGKNISSHSNI
IFSNGKLDPWSGGGVTRDITDTLVAINIPDGAHHLDLRAHNAFDPSSVLLSRLLEVKHMKKWILDFYSNI
Q

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC055022
ORF Size 1475 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC055022, AAH55022
RefSeq Size 2339 bp
RefSeq ORF 1475 bp
Locus ID 72461
Gene Summary Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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