Eif2a (BC030447) Mouse Tagged ORF Clone

CAT#: MR207798

  • TrueORF®

Eif2a (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2a (cDNA clone MGC:40963 IMAGE:5400637)

ORF Plasmid: DDK tGFP



  "BC030447" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 4,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


EIF2A Antibody - middlel region
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms D030048D22; D3Ertd194e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207798 representing BC030447
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAAACTTCTAAAGATGGCACTGCTGGGACGCCTAACCTACAACTTTATGACATGAAAACTGGAG
CATGTTTGAAATCGTTCATCCAGAAAAAAATGCAAAATTGGTGTCCTTCCTGGTCAGATGATGAAATTAT
TTGTGCCCGGAATGTTAACAACGAAGTTCACTTCTTTGAGAACAACAATTTTAACACAATTGCAAATAAA
TTACATTTGCAAAAAGTTAATGACTTTAATTTATCACCTGGAACCCAACCTTATAAGGTGGCAGTTTATG
TCCCAGGAAGTAAAGGTGCCCCCTCATTTGTTCGACTGTATCAGTATCCCAACTTTGCCGGACCTCAGGC
AGCACTGGCCAATAAAAGCTTCTTTAAAGCTGATAAGGTTACAATGCTGTGGAATAAAAAAGCTACTGCT
GTGCTGGTAATAGCCAGTACAGAGGTTGACAAGACAGGAGCCTCCTACTATGGAGAACAAACACTGCACT
ACATTGCAACAAATGGAGAAAGTGCTGTGGTACAATTACCAAAAAATGGCCCAATTTATGATGTAGTTTG
GAATTCCAGTTCTACTGAGTTTTGCGCTGTTTATGGTTTTATGCCTGCGAAAGCAACAGTTTTCAACCTG
AAGTGTGATCCTGTGTTTGACTTTGGGACTGGTCCTCGCAACGCAGCCTTCTATAGCCCTCATGGACATA
TATTAGTGCTGGCTGGATTTGGAAATCTTCGAGGACAAATGGAAGTATGGGATGTTAAAAACTACAAGCT
TATTTCTAAGCCAGTGGCTTCTGATTCTACATATTTTGCCTGGTGCCCAGATGGTGAGCATATCTTAACA
GCCACATGTGCTCCTAGGTTACGTGTTAATAATGGGTATAAGATCTGGCATTATACTGGCTCTCTTTTGC
ATAAGTATGATGTGCCATCAAATGGAGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATATT
TCCAGCAAAAACGATAAAGTACCAAGCAGTTCCAAGTGAAGTGCCTAGTGAGGAGCCTAAAGTTGCAACA
GCTTATAGACCTCCAGCCTTAAGAAATAAGCCAGTCACCAATTCCAAACTGCATGAAGAGGAACCTCCCC
AGAATATGAAGCCACATCCAGGAAGTGACAAGCCATTATCAAAAACAGCCCTTAAAAATCAAAGGAAGCA
TGAAGCTAAAAAAGCTGCAAAACAGGAAGCAAGAAGTGATGCGGCTCCTACTCCTGTCCCACAGAGTGCA
CCACGGAACACGGTCACCCAGTCTGCTTCTGGTGATCCTGAAGTAGACAAAAAAATTAAGAATCTAAAGA
AGAAACTGAAAGCCATAGAGCAGCTAAAAGAGCAGGCAGCCGCCGGGAAACAGCTAGAAAAAAATCAGTT
GGAGAAAATTCAGAAAGAGACAGCCCTTCTCCAAGAGCTTGAAGATTTGGAATTGGGAGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207798 representing BC030447
Red=Cloning site Green=Tags(s)

MEKTSKDGTAGTPNLQLYDMKTGACLKSFIQKKMQNWCPSWSDDEIICARNVNNEVHFFENNNFNTIANK
LHLQKVNDFNLSPGTQPYKVAVYVPGSKGAPSFVRLYQYPNFAGPQAALANKSFFKADKVTMLWNKKATA
VLVIASTEVDKTGASYYGEQTLHYIATNGESAVVQLPKNGPIYDVVWNSSSTEFCAVYGFMPAKATVFNL
KCDPVFDFGTGPRNAAFYSPHGHILVLAGFGNLRGQMEVWDVKNYKLISKPVASDSTYFAWCPDGEHILT
ATCAPRLRVNNGYKIWHYTGSLLHKYDVPSNGELWQVSWQPFLDGIFPAKTIKYQAVPSEVPSEEPKVAT
AYRPPALRNKPVTNSKLHEEEPPQNMKPHPGSDKPLSKTALKNQRKHEAKKAAKQEARSDAAPTPVPQSA
PRNTVTQSASGDPEVDKKIKNLKKKLKAIEQLKEQAAAGKQLEKNQLEKIQKETALLQELEDLELGV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC030447
ORF Size 1461 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC030447.1
RefSeq Size 1984 bp
RefSeq ORF 1463 bp
Locus ID 229317
MW 72.7 kDa
Gene Summary Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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