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Eif2a (BC030447) Mouse Tagged ORF Clone
CAT＃: MG207798
- TrueORF®
Eif2a (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2a (cDNA clone MGC:40963 IMAGE:5400637)
ORF Plasmid: DDK
"BC030447" in other vectors (3)
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CNY 5,320.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D030048D22; D3Ertd194e |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207798 representing BC030447
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAAACTTCTAAAGATGGCACTGCTGGGACGCCTAACCTACAACTTTATGACATGAAAACTGGAG CATGTTTGAAATCGTTCATCCAGAAAAAAATGCAAAATTGGTGTCCTTCCTGGTCAGATGATGAAATTAT TTGTGCCCGGAATGTTAACAACGAAGTTCACTTCTTTGAGAACAACAATTTTAACACAATTGCAAATAAA TTACATTTGCAAAAAGTTAATGACTTTAATTTATCACCTGGAACCCAACCTTATAAGGTGGCAGTTTATG TCCCAGGAAGTAAAGGTGCCCCCTCATTTGTTCGACTGTATCAGTATCCCAACTTTGCCGGACCTCAGGC AGCACTGGCCAATAAAAGCTTCTTTAAAGCTGATAAGGTTACAATGCTGTGGAATAAAAAAGCTACTGCT GTGCTGGTAATAGCCAGTACAGAGGTTGACAAGACAGGAGCCTCCTACTATGGAGAACAAACACTGCACT ACATTGCAACAAATGGAGAAAGTGCTGTGGTACAATTACCAAAAAATGGCCCAATTTATGATGTAGTTTG GAATTCCAGTTCTACTGAGTTTTGCGCTGTTTATGGTTTTATGCCTGCGAAAGCAACAGTTTTCAACCTG AAGTGTGATCCTGTGTTTGACTTTGGGACTGGTCCTCGCAACGCAGCCTTCTATAGCCCTCATGGACATA TATTAGTGCTGGCTGGATTTGGAAATCTTCGAGGACAAATGGAAGTATGGGATGTTAAAAACTACAAGCT TATTTCTAAGCCAGTGGCTTCTGATTCTACATATTTTGCCTGGTGCCCAGATGGTGAGCATATCTTAACA GCCACATGTGCTCCTAGGTTACGTGTTAATAATGGGTATAAGATCTGGCATTATACTGGCTCTCTTTTGC ATAAGTATGATGTGCCATCAAATGGAGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATATT TCCAGCAAAAACGATAAAGTACCAAGCAGTTCCAAGTGAAGTGCCTAGTGAGGAGCCTAAAGTTGCAACA GCTTATAGACCTCCAGCCTTAAGAAATAAGCCAGTCACCAATTCCAAACTGCATGAAGAGGAACCTCCCC AGAATATGAAGCCACATCCAGGAAGTGACAAGCCATTATCAAAAACAGCCCTTAAAAATCAAAGGAAGCA TGAAGCTAAAAAAGCTGCAAAACAGGAAGCAAGAAGTGATGCGGCTCCTACTCCTGTCCCACAGAGTGCA CCACGGAACACGGTCACCCAGTCTGCTTCTGGTGATCCTGAAGTAGACAAAAAAATTAAGAATCTAAAGA AGAAACTGAAAGCCATAGAGCAGCTAAAAGAGCAGGCAGCCGCCGGGAAACAGCTAGAAAAAAATCAGTT GGAGAAAATTCAGAAAGAGACAGCCCTTCTCCAAGAGCTTGAAGATTTGGAATTGGGAGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207798 representing BC030447
Red=Cloning site Green=Tags(s) MEKTSKDGTAGTPNLQLYDMKTGACLKSFIQKKMQNWCPSWSDDEIICARNVNNEVHFFENNNFNTIANK LHLQKVNDFNLSPGTQPYKVAVYVPGSKGAPSFVRLYQYPNFAGPQAALANKSFFKADKVTMLWNKKATA VLVIASTEVDKTGASYYGEQTLHYIATNGESAVVQLPKNGPIYDVVWNSSSTEFCAVYGFMPAKATVFNL KCDPVFDFGTGPRNAAFYSPHGHILVLAGFGNLRGQMEVWDVKNYKLISKPVASDSTYFAWCPDGEHILT ATCAPRLRVNNGYKIWHYTGSLLHKYDVPSNGELWQVSWQPFLDGIFPAKTIKYQAVPSEVPSEEPKVAT AYRPPALRNKPVTNSKLHEEEPPQNMKPHPGSDKPLSKTALKNQRKHEAKKAAKQEARSDAAPTPVPQSA PRNTVTQSASGDPEVDKKIKNLKKKLKAIEQLKEQAAAGKQLEKNQLEKIQKETALLQELEDLELGV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC030447 |
| ORF Size | 1461 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC030447.1 |
| RefSeq Size | 1984 bp |
| RefSeq ORF | 1463 bp |
| Locus ID | 229317 |
| Gene Summary | Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR207798 | Eif2a (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2a (cDNA clone MGC:40963 IMAGE:5400637) |
CNY 4,850.00 |
|
| MR207798L3 | Lenti ORF clone of Eif2a (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2a (cDNA clone MGC:40963 IMAGE:5400637) |
CNY 6,750.00 |
|
| MR207798L4 | Lenti ORF clone of Eif2a (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2a (cDNA clone MGC:40963 IMAGE:5400637) |
CNY 6,750.00 |
