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Sp100 (BC069183) Mouse Tagged ORF Clone
CAT＃: MR207411
- TrueORF®
Sp100 (Myc-DDK-tagged) - Mouse nuclear antigen Sp100 (cDNA clone MGC:70218 IMAGE:4911025)
ORF Plasmid: tGFP
"BC069183" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A430075G10Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207411 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGGAGCGACGGCAGCCCCAGGATGTCCACTGAACAGGAGAACACAGAGATGCATCTCATTGAAT GTATGTTAAAGCACTTCAAGACACAGAAAGTGGCCATTTCTAATGCGATAAGAAGTACATTTCCTTTTCT TGAGAGCCTCCGAGACCATGAATTCATCACTGGTAAAATGTATGAAGATTTGCTAGATTCCTGTAGATCG CTGGTCCCTGTGGACAAAGTGATCTACAGAGCCCTGGAGGAACTGGAGAAGAAATTTGACATGACGGTAC TGTGCGAATTGTTCAATGAGGTCAACATGGAGAAGTACCCCGATTTGAATCTCATTCGTAGAAGCTTTGG ATGTGTGTTTCCCAATGAATTGTGTTTCCAAGGAATTGATGGAGGGAACCCAAACTCCCAGCTAAGTCTT GAACAAGGACCTGGTGCCAGCTATTCTCAAGGAAGCCCGAATGGGTCATCCTTAGATCTCTCCGCATCTG AAGGCTGGAGAAGTAATGATAGAAGAAATTCCAACCTCATGCAGGCAAACCAAACTGAGAATCATCAGCT TGCCGAATCACCAGGTCACCTTGATTCCTGTGAACTCCAAGTCCAACTGAATAACAGAGATGCTACCCCA GAGTCTTGCAGCCTATTGCCTCAGAATGAAGAAAGAGCTGTGCAACTCAACTATGAACTCCAGATTAATC CCTGTTTTGTGCAACTGGTAGATGTAAAGAAGGAAAATTCATCATTTTCCTTGGCTGGTAACCAACAAAC CCGAGCAAGGACCAACCAAAATGAGGACTCTGAGATAATAGAACTCAGCAGTGGGGATTCTGATAATGGA GAGAACTTCTCAGAAGCTACAACCACAGTCCCCTCCCAGCCAGCACCTGCATATTCCAGAAAACCTCCAA CACTGAGAAGAGACAGAGGAGGAGGGAAAAAGAGAGAGAGACTTGGAAGCTACTTAATACGGAACATCAA AATCCCAATGAAACCAAGCTGGAAAACAGCTTTCCTAGCAAGAAGTGCCAATCCTTCTTCACAACGACGC AGAAAAAGAGGTCCGAGAATCCCCAGAGAAGAAAATGCGGATTTTGGAGGAGCTGAGCTTCCTGTGGTAT GTGGCAACGCTCAGGGCTTCCTGGATAAGGAGAAATTTAAGCAAGGCATCTATGTGAGGAGTATACGGGG CAAGACAGGGCGGTTATTCACTCCCATGGACTTTGAAATCGAAGGAAACTGTGAAAAAGCCAAGAACTGG CGGCAAAGCATCCGCTGCAAGGGATGGACCCTGAGAGAACTGATACAGAAAGGAGTACTACAAGACCCAC CAAGGAAAAAGAAGGAAACTCCACGAAACCCAAGGCAAACAAGGAGACAGGTAAATGCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207411 protein sequence
Red=Cloning site Green=Tags(s) MEGSDGSPRMSTEQENTEMHLIECMLKHFKTQKVAISNAIRSTFPFLESLRDHEFITGKMYEDLLDSCRS LVPVDKVIYRALEELEKKFDMTVLCELFNEVNMEKYPDLNLIRRSFGCVFPNELCFQGIDGGNPNSQLSL EQGPGASYSQGSPNGSSLDLSASEGWRSNDRRNSNLMQANQTENHQLAESPGHLDSCELQVQLNNRDATP ESCSLLPQNEERAVQLNYELQINPCFVQLVDVKKENSSFSLAGNQQTRARTNQNEDSEIIELSSGDSDNG ENFSEATTTVPSQPAPAYSRKPPTLRRDRGGGKKRERLGSYLIRNIKIPMKPSWKTAFLARSANPSSQRR RKRGPRIPREENADFGGAELPVVCGNAQGFLDKEKFKQGIYVRSIRGKTGRLFTPMDFEIEGNCEKAKNW RQSIRCKGWTLRELIQKGVLQDPPRKKKETPRNPRQTRRQVNAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC069183 |
| ORF Size | 1392 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC069183, AAH69183 |
| RefSeq Size | 1665 bp |
| RefSeq ORF | 1394 bp |
| Locus ID | 20684 |
| MW | 52.6 kDa |
| Gene Summary | Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. May also play a role in infection by viruses through mechanisms that may involve chromatin and/or transcriptional regulation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217750 | Sp100 (untagged) - Mouse nuclear antigen Sp100 (cDNA clone MGC:70218 IMAGE:4911025), (10ug) |
CNY 6,940.00 |
|
| MG207411 | Sp100 (tGFP-tagged) - Mouse nuclear antigen Sp100 (cDNA clone MGC:70218 IMAGE:4911025) |
CNY 2,950.00 |
|
| MR207411L3 | Lenti ORF clone of Sp100 (Myc-DDK-tagged) - Mouse nuclear antigen Sp100 (cDNA clone MGC:70218 IMAGE:4911025) |
CNY 4,560.00 |
|
| MR207411L4 | Lenti ORF clone of Sp100 (mGFP-tagged) - Mouse nuclear antigen Sp100 (cDNA clone MGC:70218 IMAGE:4911025) |
CNY 4,560.00 |
