Sp100 (BC069183) Mouse Untagged Clone

CAT#: MC217750

Sp100 (untagged) - Mouse nuclear antigen Sp100 (cDNA clone MGC:70218 IMAGE:4911025), (10ug)



  "BC069183" in other vectors (4)

CNY 6,940.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
SP100 Antibody - C-terminal region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms A430075G10Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC069183
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGGGAGCGACGGCAGCCCCAGGATGTCCACTGAACAGGAGAACACAGAGATGCATCTCATTGAAT
GTATGTTAAAGCACTTCAAGACACAGAAAGTGGCCATTTCTAATGCGATAAGAAGTACATTTCCTTTTCT
TGAGAGCCTCCGAGACCATGAATTCATCACTGGTAAAATGTATGAAGATTTGCTAGATTCCTGTAGATCG
CTGGTCCCTGTGGACAAAGTGATCTACAGAGCCCTGGAGGAACTGGAGAAGAAATTTGACATGACGGTAC
TGTGCGAATTGTTCAATGAGGTCAACATGGAGAAGTACCCCGATTTGAATCTCATTCGTAGAAGCTTTGG
ATGTGTGTTTCCCAATGAATTGTGTTTCCAAGGAATTGATGGAGGGAACCCAAACTCCCAGCTAAGTCTT
GAACAAGGACCTGGTGCCAGCTATTCTCAAGGAAGCCCGAATGGGTCATCCTTAGATCTCTCCGCATCTG
AAGGCTGGAGAAGTAATGATAGAAGAAATTCCAACCTCATGCAGGCAAACCAAACTGAGAATCATCAGCT
TGCCGAATCACCAGGTCACCTTGATTCCTGTGAACTCCAAGTCCAACTGAATAACAGAGATGCTACCCCA
GAGTCTTGCAGCCTATTGCCTCAGAATGAAGAAAGAGCTGTGCAACTCAACTATGAACTCCAGATTAATC
CCTGTTTTGTGCAACTGGTAGATGTAAAGAAGGAAAATTCATCATTTTCCTTGGCTGGTAACCAACAAAC
CCGAGCAAGGACCAACCAAAATGAGGACTCTGAGATAATAGAACTCAGCAGTGGGGATTCTGATAATGGA
GAGAACTTCTCAGAAGCTACAACCACAGTCCCCTCCCAGCCAGCACCTGCATATTCCAGAAAACCTCCAA
CACTGAGAAGAGACAGAGGAGGAGGGAAAAAGAGAGAGAGACTTGGAAGCTACTTAATACGGAACATCAA
AATCCCAATGAAACCAAGCTGGAAAACAGCTTTCCTAGCAAGAAGTGCCAATCCTTCTTCACAACGACGC
AGAAAAAGAGGTCCGAGAATCCCCAGAGAAGAAAATGCGGATTTTGGAGGAGCTGAGCTTCCTGTGGTAT
GTGGCAACGCTCAGGGCTTCCTGGATAAGGAGAAATTTAAGCAAGGCATCTATGTGAGGAGTATACGGGG
CAAGACAGGGCGGTTATTCACTCCCATGGACTTTGAAATCGAAGGAAACTGTGAAAAAGCCAAGAACTGG
CGGCAAAGCATCCGCTGCAAGGGATGGACCCTGAGAGAACTGATACAGAAAGGAGTACTACAAGACCCAC
CAAGGAAAAAGAAGGAAACTCCACGAAACCCAAGGCAAACAAGGAGACAGGTAAATGCCCTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-MluI     
ACCN BC069183
Insert Size 1395 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC069183, AAH69183
RefSeq Size 1665 bp
RefSeq ORF 1394 bp
Locus ID 20684
Gene Summary Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. May also play a role in infection by viruses through mechanisms that may involve chromatin and/or transcriptional regulation (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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