Usp14 (NM_001038589) Mouse Tagged ORF Clone
CAT#: MR207319
- TrueORF®
Usp14 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 14 (Usp14), transcript variant 2
ORF Plasmid: tGFP
"NM_001038589" in other vectors (4)
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CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2610005K12Rik; 2610037B11Rik; AW107924; ax; C78769; nmf375 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207319 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACTCTACTCTGTTACAGTAAAATGGGGAAAGGAGAAGTTTGAAGGTGTAGAATTGAATACTGATG AACCTCCAATGGTGTTCAAAGCCCAGCTTTTTGCATTGACTGGAGTCCAGCCAGCCAGACAAAAAGTTAT GGTGAAAGGAGGAACCCTGAAGGATGATGACTGGGGAAACATCAAAATGAAAAATATGGAATTGCCATGT GGATTGACAAACCTTGGTAACACTTGTTACATGAATGCTACAGTTCAGTGTATTCGTTCTGTGCCTGAAC TCAAAGATGCCCTTAAAAGGTATGCAGGTGCGTTGAGAGCTTCAGGAGAAATGGCTTCAGCACAGTATAT TACTGCAGCTCTTAGAGATTTGTTTGATTCCATGGATAAAACTTCTTCTAGTATCCCACCTATTATTCTG CTGCAGTTTCTGCACATGGCCTTTCCACAGTTTGCAGAGAAGGGCGAACAAGGGCAGTATCTTCAGCAGG ATGCTAACGAATGCTGGATACAAATGATGAGAGTCCTGCAACAGAAACTAGAAGCCATAGAGGATGATTC TGGTAGAGAGACGGATTCTTCATCTGCACCAGCGGTGACACCTTCTAAAAAGAAAAGCTTAATTGATCAA TATTTTGGTGTTGAGTTTGAAACTACCATGAAATGTACAGAATCTGAAGAAGAAGAGGTCACCAAAGGAA AGGAAAATCAACTTCAACTTAGTTGTTTCATTAACCAGGAAGTGAAGTATCTTTTCACAGGGCTGAAATT GCGACTTCAGGAAGAAATTACTAAACAGTCTCCAACACTGCAGAGAAATGCTTTGTACATCAAATCTTCT AAGATCAGCCGGCTACCTGCTTACTTAACTATTCAAATGGTTCGATTTTTTTATAAAGAGAAAGAATCTG TGAACGCCAAAGTTCTTAAGGATGTTAAGTTTCCTCTTATGTTGGATGTGTATGAACTCTGCACACCAGA ACTTCAAGAGAAAATGGTCTCTTTTCGGTCAAAATTCAAGGATCTAGAAGATAAAAAAGTGAATCAGCAA CCAAATGCAAATGACAAGAATAGTCCTCCGAAAGAGATTAAGTATGAACCCTTTTCATTTGCTGACGATA TTGGCTCCAATAATTGTGGCTACTATGACTTACAAGCAGTGTTAACTCACCAGGGAAGATCTAGTTCTTC AGGTCATTATGTATCATGGGTGAGAAGAAAACAAGATGAATGGATCAAATTTGATGATGATAAGGTCAGC ATTGTAACCCCAGAAGATATTCTGCGGCTGTCTGGTGGTGGAGACTGGCACATTGCTTATGTTCTACTTT ATGGGCCTCGAAGAGTTGAAATAATGGAAGAAGAAAGTGAACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207319 protein sequence
Red=Cloning site Green=Tags(s) MPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKMKNMELPC GLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIIL LQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSGRETDSSSAPAVTPSKKKSLIDQ YFGVEFETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSS KISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPLMLDVYELCTPELQEKMVSFRSKFKDLEDKKVNQQ PNANDKNSPPKEIKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVRRKQDEWIKFDDDKVS IVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001038589 |
ORF Size | 1374 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001038589.2, NP_001033678.1 |
RefSeq Size | 4080 bp |
RefSeq ORF | 1377 bp |
Locus ID | 59025 |
MW | 52.3 kDa |
Gene Summary | Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204754 | Usp14 (untagged) - Mouse ubiquitin specific peptidase 14 (Usp14), transcript variant 2, (10ug) |
CNY 3,656.00 |
|
MG207319 | Usp14 (tGFP-tagged) - Mouse ubiquitin specific peptidase 14 (Usp14), transcript variant 2 |
CNY 5,040.00 |
|
MR207319L3 | Lenti ORF clone of Usp14 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 14 (Usp14), transcript variant 2 |
CNY 6,460.00 |
|
MR207319L4 | Lenti ORF clone of Usp14 (mGFP-tagged) - Mouse ubiquitin specific peptidase 14 (Usp14), transcript variant 2 |
CNY 6,460.00 |