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Myef2 (BC060946) Mouse Tagged ORF Clone
CAT＃: MR207253
- TrueORF®
Myef2 (Myc-DDK-tagged) - Mouse myelin basic protein expression factor 2, repressor (cDNA clone MGC:74034 IMAGE:6741255)
ORF Plasmid: tGFP
"BC060946" in other vectors (4)
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CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9430071B01 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207253 representing BC060946
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAATGATGAGTCAGTGAAGGAAGAAAAATCTGATTTAAAAGAAAAGTCTACAGGAAATAAGAAGG CCAATCGGTTCCATCCTTATTCAAAAGATAAGAATTCGGGCACTGGAGAAAAGAAAGGTCCAAATCGGAA CAGAGTGTTCATTAGCAACATACCCTATGACATGAAGTGGCAGGCTATTAAAGATTTGATGAGAGAAAAA GTTGGTGAGGTTACATACGTGGAGCTCTTTAAGGATGCGGAAGGAAAATCAAGGGGTTGTGGCGTGGTTG AATTCAAAGATGAAGAATTTGTAAAGAAAGCATTAGAAACTATGAACAAATATGACCTTAGTGGAAGGCC TTTGAATATTAAAGAGGATCCGGATGGAGAAAATGCTCGCCGGGCACTGCAGCGAACAGGAACATCATTT CAAGGATCACATGCCTCCGATGTGGGATCTGGGTTGGTGAATTTACCACCTTCCATTCTCAATAATCCAA ACATTCCTCCTGAGGTTATCAGCAATTTGCAGGCTGGTAGACTTGGTTCCACAATTTTTGTTGCTAATCT TGACTTCAAAGTTGGTTGGAAGAAATTAAAGGAAGTGTTCAGCATAGCTGGAACTGTGAAGCGAGCTGAT ATTAAAGAAGACAAGGATGGCAAGAGCAGAGGCATGGGCACTGTCACTTTTGAGCAGGCAATTGAAGCAG TCCAAGCAATTTCCATGTTCAATGGGCAGTTTTTATTTGATAGACCTATGCATGTGAAAATGGATGACAA ATCTGTCCCTCATGAAGACTACCGTTCACATGATAGTAAGACATCACAGTTACCACGTGGTCTTGGAGGC ATTGGAATGGGACTTGGTCCAGGTGGACAGCCTATTAGTGCCAGCCAGCTGAACATAACTGGTGTAATGG GAAATTTGGGTCCAAGTGGTATGGGAATGGATGGTCCGGGTTTTGGAGGAGTGAATAGAATTGGAGGAGG AGTTGGGTTTGGTGGTCTGGAAGCAATGAATAGCATGGCAGGCTTTGGTGGAGTTGGCCGAATGGGAGGC GGCAGCATGAGCGGCATGAGCACTGTGACTGGAGGCATGGGCATGGGACTGGACCGCATGAGCTCCAGCT TCGACAGGATGGGGCCAGGCATTGGAGCCATACTGGAAAGGAGCATCGATGTAGACCGAGGGTTTTTATC AGGTCCCATGGGAAGCGGAATGAGAGACAGATTAGGCTCCAAAGGCAACCAGATATTTGTTAGAAATCTT CCTTTTGACTTGACTTGGCAGAAATTAAAAGAGAAATTCAGCCAATGTGGCCAGATTAACAGAGATAGCA AGACTCACTCTAACTGTGGACAACACCATTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207253 representing BC060946
Red=Cloning site Green=Tags(s) MENDESVKEEKSDLKEKSTGNKKANRFHPYSKDKNSGTGEKKGPNRNRVFISNIPYDMKWQAIKDLMREK VGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENARRALQRTGTSF QGSHASDVGSGLVNLPPSILNNPNIPPEVISNLQAGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRAD IKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVPHEDYRSHDSKTSQLPRGLGG IGMGLGPGGQPISASQLNITGVMGNLGPSGMGMDGPGFGGVNRIGGGVGFGGLEAMNSMAGFGGVGRMGG GSMSGMSTVTGGMGMGLDRMSSSFDRMGPGIGAILERSIDVDRGFLSGPMGSGMRDRLGSKGNQIFVRNL PFDLTWQKLKEKFSQCGQINRDSKTHSNCGQHHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC060946 |
| ORF Size | 1362 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060946.1 |
| RefSeq Size | 1692 bp |
| RefSeq ORF | 1364 bp |
| Locus ID | 17876 |
| MW | 62 kDa |
| Gene Summary | Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218204 | Myef2 (untagged) - Mouse myelin basic protein expression factor 2, repressor (cDNA clone MGC:74034 IMAGE:6741255), (10ug) |
CNY 6,940.00 |
|
| MG207253 | Myef2 (tGFP-tagged) - Mouse myelin basic protein expression factor 2, repressor (cDNA clone MGC:74034 IMAGE:6741255) |
CNY 5,040.00 |
|
| MR207253L3 | Lenti ORF clone of Myef2 (Myc-DDK-tagged) - Mouse myelin basic protein expression factor 2, repressor (cDNA clone MGC:74034 IMAGE:6741255) |
CNY 6,460.00 |
|
| MR207253L4 | Lenti ORF clone of Myef2 (mGFP-tagged) - Mouse myelin basic protein expression factor 2, repressor (cDNA clone MGC:74034 IMAGE:6741255) |
CNY 6,460.00 |
